Mechanics of the cell: Interaction mechanisms and mechanobiological models

Liebman, Caleb; McColloch, Andrew; Rabiei, Manoochehr; Bowling, Alan; Cho, Michael

doi:10.1016/bs.ctm.2020.09.001

Cited by 8 publications

(5 citation statements)

References 165 publications

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“…This tension on the plasma membrane is related primarily to the connection between the membrane and ECM and its internal cytoskeletal networks [ 28 ]. Measuring the mechanical coupling between two cell membrane tethers at distances of 5–15 μm exhibits no long-distance propagation of the cell membrane at a short scale, probably due to the membrane flow disturbance being mediated by the interaction between transmembrane proteins and the underlying cytoskeleton [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Visualization of Cell Membrane Tension Regulated by the Microfilaments as a “Shock Absorber” in Micropatterned Cells

Wang

Zhang

et al. 2023

Biology

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The extracellular stress signal transmits along the cell membrane–cytoskeleton–focal adhesions (FAs) complex, regulating the cell function through membrane tension. However, the mechanism of the complex regulating membrane tension is still unclear. This study designed polydimethylsiloxane stamps with specific shapes to change the actin filaments’ arrangement and FAs’ distribution artificially in live cells, visualized the membrane tension in real time, and introduced the concept of information entropy to describe the order degree of the actin filaments and plasma membrane tension. The results showed that the actin filaments’ arrangement and FAs’ distribution in the patterned cells were changed significantly. The hypertonic solution resulted in the plasma membrane tension of the pattern cell changing more evenly and slowly in the zone rich in cytoskeletal filaments than in the zone lacking filaments. In addition, the membrane tension changed less in the adhesive area than in the non-adhesive area when destroying the cytoskeletal microfilaments. This suggested that patterned cells accumulated more actin filaments in the zone where FAs were difficult to generate to maintain the stability of the overall membrane tension. The actin filaments act as shock absorbers to cushion the alternation in membrane tension without changing the final value of membrane tension.

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Section: Discussionmentioning

confidence: 99%

Visualization of Cell Membrane Tension Regulated by the Microfilaments as a “Shock Absorber” in Micropatterned Cells

Wang

Zhang

et al. 2023

Biology

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“…In a similar manner to the microtubules, they exhibit a polymeric nature, being formed by monomers of globular actin (G-actin). The helical aggregation of these proteins occurs after their binding with ATP molecules, and it results in the formation of short chains of F-actin, which, with the aid of proteins, acquire stabilization [ 283 ]. Tolytoxin exhibits cytostatic effects against various cell types; among them are breast, human epidermoid, and ovarian carcinoma cells, as well as neural cells [ 74 , 273 ].…”

Section: Major Targetsmentioning

confidence: 99%

Cyanobacteria: A Promising Source of Antifungal Metabolites

et al. 2023

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Cyanobacteria are a rich source of secondary metabolites, and they have received a great deal of attention due to their applicability in different industrial sectors. Some of these substances are known for their notorious ability to inhibit fungal growth. Such metabolites are very chemically and biologically diverse. They can belong to different chemical classes, including peptides, fatty acids, alkaloids, polyketides, and macrolides. Moreover, they can also target different cell components. Filamentous cyanobacteria have been the main source of these compounds. This review aims to identify the key features of these antifungal agents, as well as the sources from which they are obtained, their major targets, and the environmental factors involved when they are being produced. For the preparation of this work, a total of 642 documents dating from 1980 to 2022 were consulted, including patents, original research, review articles, and theses.

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“…Both mechanisms collect the activity of several intracellular and extracellular components (Table 1) that, working together in a spatial-temporal manner, transmit the signaling to the cell DNA and change the cell gene expression (Trubelja and Bao 2018;Martino et al 2018;Argentati et al 2019;Janota et al 2020). The most known pathways include (i) integrins-ECM-Focal adhesion (FAs) complexes-cytoskeleton-nucleoskeleton proteins (Weinberg et al 2017;Jansen et al 2017;Morena et al 2017;Martino et al 2018;Luzi et al 2020;Argentati et al 2021); (ii) Adherens Junctions (AJs) complexes for cell-cell interaction-cytoskeleton-nucleoskeleton proteins (Morena et al 2017;Martino et al 2018;Yap et al 2018;Liebman et al 2020). The overall interconnection also influences the behavior of neighboring cells and can remodel constantly the ECM environment through synthesis, degradation, and chemical modification processes (Humphrey et al 2014;Stanton et al 2019;Argentati et al 2019).…”

Section: Pills Of Mechanobiologymentioning

confidence: 99%

The role of physical cues in the development of stem cell-derived organoids

et al. 2021

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Organoids are a novel three-dimensional stem cells’ culture system that allows the in vitro recapitulation of organs/tissues structure complexity. Pluripotent and adult stem cells are included in a peculiar microenvironment consisting of a supporting structure (an extracellular matrix (ECM)-like component) and a cocktail of soluble bioactive molecules that, together, mimic the stem cell niche organization. It is noteworthy that the balance of all microenvironmental components is the most critical step for obtaining the successful development of an accurate organoid instead of an organoid with heterogeneous morphology, size, and cellular composition. Within this system, mechanical forces exerted on stem cells are collected by cellular proteins and transduced via mechanosensing—mechanotransduction mechanisms in biochemical signaling that dictate the stem cell specification process toward the formation of organoids. This review discusses the role of the environment in organoids formation and focuses on the effect of physical components on the developmental system. The work starts with a biological description of organoids and continues with the relevance of physical forces in the organoid environment formation. In this context, the methods used to generate organoids and some relevant published reports are discussed as examples showing the key role of mechanosensing–mechanotransduction mechanisms in stem cell-derived organoids.

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Mechanics of the cell: Interaction mechanisms and mechanobiological models

Cited by 8 publications

References 165 publications

Visualization of Cell Membrane Tension Regulated by the Microfilaments as a “Shock Absorber” in Micropatterned Cells

Visualization of Cell Membrane Tension Regulated by the Microfilaments as a “Shock Absorber” in Micropatterned Cells

Cyanobacteria: A Promising Source of Antifungal Metabolites

The role of physical cues in the development of stem cell-derived organoids

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