Mechanism analysis of octapeptide from microalgae, Isochrysis zhanjiangensis for suppressing vascular injury and angiogenesis in human umbilical vein endothelial cell

Marine Microalgal Products with Activities against Age-Related Cardiovascular Diseases

“…The peptide could reduce cell damage by increasing the expression of antioxidant enzymes, such as the antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase-1 (GPX), and haem oxygenase 1 (HO-1). In addition, it also inhibited pro-inflammatory mediators tumor necrosis factor (TNF)-α, cytokine interleukin-6 (IL-6), and ICAM-1 [30] (Table 1). Vo et al in 2013 [31] isolated two peptides with aminoacidic sequences of LDAVNR for peptide 1 and MMLDF for peptide 2 from the peptidic hydrolysates of Spirulina maxima.…”

“…In addition to polysaccharides, peptides can counteract the pathological processes by mimicking the function of mediators or modulating the activities and expression of mediators involved in hypertension, hypercholesterolemia, diabetes, inflammation and oxidative stress [29]. Lin et al [30] studied Isochrysis zhanjiangensis, which was suggested to inhibit vascular injury and angiogenesis, and to have a protective effect on CVDs. They characterized the production and the activity of an octapeptide (ICE) isolated from I. zhanjiangensis, demonstrating that ICE was able to decrease ROS production in lipopolysaccharide (LPS)-induced HUVECs (concentrations of ICE 1, 10, 20, and 50 µM).…”

A novel peptide derived fromHaematococcus pluvialisresidue exhibits anti-aging activity inCaenorhabditis elegans viathe insulin/IGF-1 signaling pathway

“…For example, the octapeptide from Isochrysis zhanjiangensis (IIAVEAGGC) alleviated lipopolysaccharide-induced oxidative stress of human umbilical vein endothelial cell by inhibiting the protein phosphorylation expression of PI3K and P38, which were the key factors in IIS and MAPK pathways, respectively. 18 Accordingly, this study hypothesizes that the H. pluvialis peptide could prolong the lifespan of C. elegans via the IIS pathway and/or MAPK pathway. In the present study, we used C. elegans as an in vivo model to systematically investigate the longevity effect and mechanism of HPp for the first time, determining the crucial indicators, e.g., survival, lipofuscin, motility, and stress resistance.…”

“…For example, the octapeptide from Isochrysis zhanjiangensis (IIAVEAGGC) alleviated lipopolysaccharide-induced oxidative stress of human umbilical vein endothelial cell by inhibiting the protein phosphorylation expression of PI3K and P38, which were the key factors in IIS and MAPK pathways, respectively. 18…”

Comparativein silicoandin vitrostudy of the stability and biological activity of an octapeptide from microalgaeIsochrysis zhanjiangensisand its truncated short peptide

“…6,7 Previously, we identified antioxidant peptides IIAVEAGC (774.92 Da) from the microalgae Isochrysis zhanjiangensis. 8 At present, Isochrysis zhanjiangensis is one of the high quality microalgae and has been widely used. 9 However, current research on Isochrysis zhanjiangensis mainly focuses on how to improve the yield 10 and the impact of the environment on its growth or yield.…”