Mechanism and kinetics of transfer of a fluorescent fatty acid between single-walled phosphatidylcholine vesicles

Doody, Michael C.; Pownall, Henry J.; Kao, Yin J.; Smith, Louis C.

doi:10.1021/bi00542a017

Cited by 191 publications

(153 citation statements)

References 23 publications

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“…Such effects were first shown for phosphatidylglycerol [110] and later studied in detail for fatty acid interbilayer transfer by Smith, Pownall and coworkers [114][115]. These investigators showed that increasing the ionic strength of the aqueous medium slowed the interbilayer transfer of pyrene-labeled fatty acids.…”

Section: Iii-d Aqueous Mileumentioning

confidence: 92%

“…Nearly concurrent with the discovery that phospholipids undergo spontaneous interbilayer transfer, the importance of the lipid bilayers phase state on the kinetics of this process was recognized [110][111][112][113][114][115][116]. The details of these intial pioneering studies have been reviewed thoroughly by Lange [3].…”

Section: Iii-a Lamellar Physical Statementioning

confidence: 99%

“…The mechanism by which spontaneous lipid transfer occurs between membranes has been investigated in many independent laboratories [23,26,57,66,[110][111][112][113][114][132][133][134][135]. In the vast majority of cases, it has been concluded that the transfer event is a first-order process in which the rate-limiting step is desorption of lipid molecules from the donor membrane surface followed by rapid diffusion through the aqueous phase to an acceptor membrane (Fig.…”

Section: What Is the Mechanism Of Spontaneous Lipid Transfer? Iv-mentioning

confidence: 99%

“…Thermodynamic analysis of the activated state and of fatty acid partitioning indicates that the rate-limiting step is formation of an aqueous intermediate in the interfacial region [114]. Moreover, fatty acid transfer kinetics are not dictated by only the partition equilibirum, but are also influenced by the solvation properties of the interfacial water associated with the vesicles [114]. Comparison of the fatty acid transfer rates and activation energies with those of corresponding methyl ester, alcohol, and alkane derivatives has yielded further insights [115].…”

Section: What Is the Mechanism Of Spontaneous Lipid Transfer? Iv-mentioning

confidence: 99%

“…However, as was pointed out above, the majority of experimental data reported prior to 1988 argued against such a mechanism of lipid transfer (e.g., Refs. 23,26,57,66,[110][111][112][113][114][132][133][134][135]. Then, two different laboratories both reported increased spontaneous lipid transfer rates attributable to transient collisions between donor and acceptor surfaces [96][97][98][107][108][109].…”

Section: Iv-b Transient Apposition Of Hydrated Membrane Surfacesmentioning

confidence: 99%

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Spontaneous lipid transfer between organized lipid assemblies

Brown

1992

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

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Section: Iii-d Aqueous Mileumentioning

confidence: 92%

Section: Iii-a Lamellar Physical Statementioning

confidence: 99%

Section: What Is the Mechanism Of Spontaneous Lipid Transfer? Iv-mentioning

confidence: 99%

Section: What Is the Mechanism Of Spontaneous Lipid Transfer? Iv-mentioning

confidence: 99%

Section: Iv-b Transient Apposition Of Hydrated Membrane Surfacesmentioning

confidence: 99%

See 3 more Smart Citations

Spontaneous lipid transfer between organized lipid assemblies

Brown

1992

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

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Fluorescence in Organized Assemblies

Hinze¹

2000

Encyclopedia of Analytical Chemistry

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This article provides an overview of the general properties of organized assembly (ordered media) systems such as aqueous surfactant and bile salt micelles, lipid and surfactant vesicles (liposomes) and cyclodextrins (CDs) and summarizes their utilization to enhance the performance of analytical fluorescence measurements. In many instances, organic molecules and metal complex species, when included within a CD cavity or solubilized and bound to surfactant aggregates, exhibit enhanced fluorescence. This gives rise to improved detectability of such analytes. The altered microenvironment within the organized medium is capable of impeding the interfering action of other species (inorganic or organic) present in the sample matrix. This often can improve the selectivity of the analytical method. These benefits of improved sensitivity and selectivity arise from the compartmentalization, isolation and shielding of the excited singlet state of the guest analyte from quenching and nonradiative decay processes as well as prevent side reactions that otherwise can occur in bulk solution (or sample matrix). In addition, organic solvents or time‐consuming extraction steps can be avoided owing to the increased solubility of the nonpolar organic or inorganic reagents and/or analyte molecules in water in the presence of the organized medium; allowing for the use of an aqueous medium to perform the procedure. The possibility of conducting reactions and forming fluorescent organic or metal chelates in micellar (or other organized) media that are not observed in a bulk homogeneous solvent system serves to expand the scope of chemistries that one can consider using to design/develop new, unique and improved fluorescent assays. Numerous representative examples of fluorescent methods for determination of both organic and inorganic analytes are provided which serve to illustrate the advantages and benefits accrued from the use of the micelles, vesicles, liposomes or CDs in such procedures. Some experimental considerations and cautions to keep in mind when utilizing organized media are also delineated. An extensive reference section is provided so that the interested reader can easily refer to such for more detailed information on these systems and topics.

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Fluorescence in Organized Assemblies

Memon¹,

Balouch²,

Hinze³

2008

Encyclopedia of Analytical Chemistry

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This article provides an overview of the general properties of organized assembly (ordered media) systems, such as aqueous surfactant and bile salt micelles, lipid (liposomes) and surfactant vesicles, and cyclodextrins (CDs), and summarizes their utilization to enhance the performance of analytical fluorescence measurements. In many instances, organic molecules and metal complex species when included within a CD cavity or solubilized and bound to surfactant aggregates exhibit enhanced fluorescence, improving detectability of such analytes. The altered microenvironment within the organized medium can impede the interfering action of other species (inorganic or organic) present in the sample matrix, which often improves the selectivity of the analytical method. These benefits of improved sensitivity and selectivity arise from the compartmentalization, isolation, and shielding of the excited singlet state of the guest analyte from quenching and nonradiative decay processes, as well as preventing side reactions that otherwise can occur in bulk solution (or sample matrix). Organic solvents or time‐consuming extraction steps can also be avoided, owing to the increased solubility of nonpolar organic or inorganic reagents and/or analyte molecules in water in the presence of the organized medium, allowing the use of an aqueous medium to perform the procedure. The possibility of conducting reactions and forming fluorescent organic or metal chelates in micellar (or other organized) media that are not observed in a bulk homogeneous solvent system serves to expand the scope of possible chemistries for design/development of new, unique, and improved fluorescent assays. Examples of fluorescent methods for determination of both organic and inorganic analytes are provided, which serve to illustrate the advantages and benefits accrued from the use of the micelles, vesicles, liposomes, or CDs in such procedures, along with experimental considerations and cautions in utilizing organized media.

show abstract

Mechanism and kinetics of transfer of a fluorescent fatty acid between single-walled phosphatidylcholine vesicles

Cited by 191 publications

References 23 publications

Spontaneous lipid transfer between organized lipid assemblies

Spontaneous lipid transfer between organized lipid assemblies

Fluorescence in Organized Assemblies

Fluorescence in Organized Assemblies

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