Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops

Ren, Wenjing; Si, Jinchao; Chen, Li; Fang, Zhiyuan; Zhuang, Mu; Lv, Honghao; Wang, Yong; Ji, Jialei; Yu, Hailong; Zhang, Yangyong

doi:10.3390/ijms23169099

Cited by 14 publications

(9 citation statements)

References 169 publications

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“…Since then, the research on important gene mining and their regulatory mechanism of B. oleracea crops has entered a period of rapid development. A series of genetic loci or genes controlling plant growth, resistance to biotic and abiotic stresses, quality composition and fertility, etc., have been excavated ( Li et al., 2021 ; Shaw et al., 2021 ; Zhang et al., 2021 ; Han et al., 2021 ; Ren et al., 2022 ; Alemán et al., 2022 ). Therefore, the effective transformation method established in this study will greatly accelerate the functional validation and regulatory mechanism study of the target genes controlling important agronomic traits of B. oleracea crops and promote rapid gene pyramiding to develop excellent target materials.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a stable, effective and universal genetic transformation technique in the diverse species of Brassica oleracea

Sheng

Wang

et al. 2022

Front. Plant Sci.

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Brassica oleracea is an economically important species, including seven cultivated variants. Agrobacterium-mediated transformation of B. oleracea crops, mainly via hypocotyl and cotyledon, has been achieved in the past. However, previously established transformation methods showed low efficiency, severe genotype limitation and a prolonged period for transformants acquisition, greatly restricting its application in functional genomic studies and crop improvement. In this study, we have compared the shoot regeneration and genetic transformation efficiency of hypocotyl, cotyledon petiole and curd peduncle explants from twelve genotypes of cauliflower and broccoli. Finally, an Agrobacterium-mediated transformation method using curd peduncle as explant was established, which is rapid, efficient, and amenable to high-throughput transformation and genome editing. The average genetic transformation efficiency of this method is stable up to 11.87% and was successfully implemented in twelve different genotypes of cauliflower and broccoli and other B. oleracea crops with low genotype dependence. Peduncle explants were found to contain abundant cambial cells with a strong cell division and shoot regeneration ability, which might be why this method achieved stable and high genetic transformation efficiency with almost no genotype dependence.

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Section: Discussionmentioning

confidence: 99%

Establishment of a stable, effective and universal genetic transformation technique in the diverse species of Brassica oleracea

Sheng

Wang

et al. 2022

Front. Plant Sci.

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“…In this system, alien cytoplasm has been obtained by crossing B. napus to Japanese radish (Raphanus sativus) (Ogura, 1968). Complete pollen abortion, ease of transfer and high progeny sterility rates, reaching 100%, are the various advantages associated with Ogura CMS system (Ren et al, 2022). This system has been extensively deployed in B. napus, B. juncea and B. oleracea (Yamagishi and Bhat, 2014).…”

Section: Somatic Hybridization and Embryo Rescuementioning

confidence: 99%

Utilization of crop wild relatives for biotic and abiotic stress management in Indian mustard [Brassica juncea (L.) Czern. & Coss.]

Verma,

Dubey,

Singh

et al. 2023

Front. Plant Sci.

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Brassica juncea (L.) Czern. & Coss. (Indian mustard) is an economically important edible oil crop. Over the years, plant breeders have developed many elite varieties of B. juncea with better yield traits, but research work on the introgression of stress resilience traits has largely been lagging due to scarcity of resistant donors. Crop wild relatives (CWRs) are the weedy relatives of domesticated plant species which are left unutilized in their natural habitat due to the presence of certain undesirable alleles which hamper their yield potential, and thus, their further domestication. CWRs of B. juncea namely include Sinapis alba L. (White mustard), B. tournefortii Gouan. (African mustard), B. fruticulosa Cirillo (Twiggy turnip), Camelina sativa L. (Gold-of-pleasure), Diplotaxis tenuisiliqua Delile (Wall rocket), D. erucoides L. (White wall rocket), D. muralis L. (Annual wall rocket), Crambe abyssinica R.E.Fr. (Abyssinian mustard), Erucastrum gallicum Willd. (Common dogmustard), E. cardaminoides Webb ex Christ (Dogmustard), Capsella bursa-pastoris L. (Shepherds purse), Lepidium sativum L. (Garden Cress) etc. These CWRs have withstood several regimes of biotic and abiotic stresses over the past thousands of years which led them to accumulate many useful alleles contributing in resistance against various environmental stresses. Thus, CWRs could serve as resourceful gene pools for introgression of stress resilience traits into Indian mustard. This review summarizes research work on the introgression of resistance against Sclerotinia stem rot (caused by Sclerotinia sclerotiorum), Alternaria blight (caused by Alternaria brassicae), white rust (caused by Albugo candida), aphid attack, drought and high temperature from CWRs into B. juncea. However, various pre- and post-fertilization barriers due to different ploidy levels are major stumbling blocks in the success of such programmes, therefore, we also insightfully discuss how the advances made in -omics technology could be helpful in assisting various breeding programmes aiming at improvisation of stress resilience traits in B. juncea.

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“…At present, Ogura CMS is the most widely used CMS type in cabbage hybrid production. It has the advantages of stable sterility and being easy transfer, and its fertility can be restored by the nuclear gene Rfo [ 7 , 11 ]. Ogura CMS was first discovered in wild radish ( Raphanus sativus ) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals a Potential Regulatory Network for Ogura Cytoplasmic Male Sterility in Cabbage (Brassica oleracea L.)

Chen

Ren

Zhang

et al. 2023

IJMS

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Ogura cytoplasmic male sterility (CMS) lines are widely used breeding materials in cruciferous crops and play important roles in heterosis utilization; however, the sterility mechanism remains unclear. To investigate the microspore development process and gene expression changes after the introduction of orf138 and Rfo, cytological observation and transcriptome analysis were performed using a maintainer line, an Ogura CMS line, and a restorer line. Semithin sections of microspores at different developmental stages showed that the degradation of tapetal cells began at the tetrad stage in the Ogura CMS line, while it occurred at the bicellular microspore stage to the tricellular microspore stage in the maintainer and restorer lines. Therefore, early degradation of tapetal cells may be the cause of pollen abortion. Transcriptome analysis results showed that a total of 1287 DEGs had consistent expression trends in the maintainer line and restorer line, but were significantly up- or down-regulated in the Ogura CMS line, indicating that they may be closely related to pollen abortion. Functional annotation showed that the 1287 core DEGs included a large number of genes related to pollen development, oxidative phosphorylation, carbohydrate, lipid, and protein metabolism. In addition, further verification elucidated that down-regulated expression of genes related to energy metabolism led to decreased ATP content and excessive ROS accumulation in the anthers of Ogura CMS. Based on these results, we propose a transcriptome-mediated induction and regulatory network for cabbage Ogura CMS. Our research provides new insights into the mechanism of pollen abortion and fertility restoration in Ogura CMS.

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Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops

Cited by 14 publications

References 169 publications

Establishment of a stable, effective and universal genetic transformation technique in the diverse species of Brassica oleracea

Establishment of a stable, effective and universal genetic transformation technique in the diverse species of Brassica oleracea

Utilization of crop wild relatives for biotic and abiotic stress management in Indian mustard [Brassica juncea (L.) Czern. & Coss.]

Comparative Transcriptome Analysis Reveals a Potential Regulatory Network for Ogura Cytoplasmic Male Sterility in Cabbage (Brassica oleracea L.)

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