Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on <i>Bacillus subtilis</i>

Nakayama, Motokazu; Shimatani, Kanami; Ozawa, Tadahiro; Shigemune, Naofumi; Tomiyama, Daisuke; Yui, Koji; Katsuki, Masahito; Ikeda, Keisuke; Nonaka, Ai; Miyamoto, Takahisa

doi:10.1080/09168451.2014.993356

Cited by 85 publications

(52 citation statements)

References 25 publications

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“…Once a membrane effect is known to exist, specific binding assays (Nakayama et al, 2015) or model membrane insertion assays (Wu et al, 2013a) can confidently test direct interactions between specific components. Model membranes have already been used to test the direct interaction of epicatechin gallate and epigallocatechin gallate with membranes (Hashimoto et al, 1999; Kumazawa et al, 2004).…”

Section: Membrane Disruption By Phenolic Compoundsmentioning

confidence: 99%

The Potential of Systems Biology to Discover Antibacterial Mechanisms of Plant Phenolics

et al. 2017

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Drug resistance of bacterial pathogens is a growing problem that can be addressed through the discovery of compounds with novel mechanisms of antibacterial activity. Natural products, including plant phenolic compounds, are one source of diverse chemical structures that could inhibit bacteria through novel mechanisms. However, evaluating novel antibacterial mechanisms of action can be difficult and is uncommon in assessments of plant phenolic compounds. With systems biology approaches, though, antibacterial mechanisms can be assessed without the bias of target-directed bioassays to enable the discovery of novel mechanism(s) of action against drug resistant microorganisms. This review article summarizes the current knowledge of antibacterial mechanisms of action of plant phenolic compounds and discusses relevant methodology.

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Section: Membrane Disruption By Phenolic Compoundsmentioning

confidence: 99%

The Potential of Systems Biology to Discover Antibacterial Mechanisms of Plant Phenolics

et al. 2017

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“…GTPs and EGCG have a broad antimicrobial spectrum and inhibit the growth of many foodborne pathogenic and spoilage bacteria [4, 11]. Several studies have indicated that the antibacterial activity of EGCG is due to damage to the bacterial cell membrane, such as peptidoglycan [12], outer membrane proteins [13] and lipid bilayers [14]. Moreover, numerous key enzymes in bacterial cells have been suggested as the targets of EGCG, including FabG and FabI reductases [15], the DNA gyrase B subunit [16], thioredoxin, and thioredoxin reductase [17].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the response of Pseudomonas fluorescens to epigallocatechin gallate by RNA-seq

Liu

Bimiao

et al. 2017

PLoS ONE

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Epigallocatechin gallate (EGCG) is a main constituent of green tea polyphenols that are widely used as food preservatives and are considered to be safe for consumption. However, the underlying antimicrobial mechanism of EGCG and the bacterial response to EGCG are not clearly understood. In the present study, a genome-wide transcriptional analysis of a typical spoilage bacterium, Pseudomonas fluorescens that responded to EGCG was performed using RNA-seq technology. A total of 26,365,414 and 23,287,092 clean reads were generated from P. fluorescens treated with or without 1 mM EGCG and the clean reads were aligned to the reference genome. Differential expression analysis revealed 291 upregulated genes and 134 downregulated genes and the differentially expressed genes (DEGs) were verified using RT-qPCR. Most of the DGEs involved in iron uptake, antioxidation, DNA repair, efflux system, cell envelope and cell-surface component synthesis were significantly upregulated by EGCG treatment, while most genes associated with energy production were downregulated. These transcriptomic changes are likely to be adaptive responses of P. fluorescens to iron limitation and oxidative stress, as well as DNA and envelope damage caused by EGCG. The expression of specific genes encoding the extra-cytoplasmic function sigma factor (PvdS, RpoE and AlgU) and the two-component sensor histidine kinase (BaeS and RpfG) were markedly changed by EGCG treatment, which may play important roles in regulating the stress responses of P. fluorescens to EGCG. The present data provides important insights into the molecular action of EGCG and the possible cross-resistance mediated by EGCG on P. fluorescens, which may ultimately contribute to the optimal application of green tea polyphenols in food preservation.

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“…Two different commercially available botanical formulations which contain Juglans nigra were also recently shown to have activity against log phase spirochetes of B. burgdorferi strain GCB726, round bodies and biofilm formation in in vitro testing (64). Juglans nigra has also been shown to have multiple constituents (65) with antimicrobial properties including juglone (5-hydroxy-1,4naphthalenedione), phenolic acids, flavonoids, and catechins (including epigallocatechin gallate) (66,67). Further studies are needed to elucidate which constituents have anti-borrelial activity.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Natural and Botanical Medicines for Activity Against Growing and Non-growing Forms of B. burgdorferi

Feng

Leone

Schweig³

et al. 2020

Front. Med.

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Lyme disease is the most common vector-borne disease in the US and Europe. Although the current recommended Lyme antibiotic treatment is effective for the majority of Lyme disease patients, about 10-20% of patients continue to suffer from persisting symptoms. There have been various anecdotal reports on the use of herbal extracts for treating patients with persisting symptoms with varying degree of improvements. However, it is unclear whether the effect of the herb products is due to their direct antimicrobial activity or their effect on host immune system. In the present study, we investigated the antimicrobial effects of 12 commonly used botanical medicines and three other natural antimicrobial agents for potential anti-Borrelia burgdorferi activity in vitro. Among them, 7 natural product extracts at 1% were found to have good activity against the stationary phase B. burgdorferi culture compared to the control antibiotics doxycycline and cefuroxime. These active botanicals include Cryptolepis sanguinolenta, Juglans nigra (Black walnut), Polygonum cuspidatum (Japanese knotweed), Artemisia annua (Sweet wormwood), Uncaria tomentosa (Cat's claw), Cistus incanus, and Scutellaria baicalensis (Chinese skullcap). In contrast, Stevia rebaudiana, Andrographis paniculata, Grapefruit seed extract, colloidal silver, monolaurin, and antimicrobial peptide LL37 had little or no activity against stationary phase B. burgdorferi. The minimum inhibitory concentration (MIC) values of Artemisia annua, Juglans nigra, and Uncaria tomentosa were quite high for growing B. burgdorferi, despite their strong activity against the non-growing stationary phase B. burgdorferi. On the other hand, the top two active herbs, Cryptolepis sanguinolenta and Polygonum cuspidatum, showed strong activity against both growing B. burgdorferi (MIC = 0.03-0.06% and 0.25-0.5%, respectively) and non-growing stationary phase B. burgdorferi. In subculture studies, only 1% Cryptolepis sanguinolenta extract caused complete eradication, while doxycycline and cefuroxime and other active herbs could not eradicate B. burgdorferi stationary phase cells as many spirochetes were visible after 21-day subculture. Further studies are needed to identify the active constituents of the effective botanicals and evaluate their combinations for more effective eradication of B. burgdorferi in vitro and in vivo. The implications of these findings for improving treatment of persistent Lyme disease are discussed.

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Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis

Cited by 85 publications

References 25 publications

The Potential of Systems Biology to Discover Antibacterial Mechanisms of Plant Phenolics

The Potential of Systems Biology to Discover Antibacterial Mechanisms of Plant Phenolics

Transcriptomic analysis of the response of Pseudomonas fluorescens to epigallocatechin gallate by RNA-seq

Evaluation of Natural and Botanical Medicines for Activity Against Growing and Non-growing Forms of B. burgdorferi

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