Mechanism Insights of Antibacterial Surfaces Coated with Dead Probiotics

Zhang, Weixiong; Qian, Lu; He, Bingen; Gong, Xiangjun; Zhang, Guangzhao

doi:10.1021/acs.langmuir.3c01818

Cited by 2 publications

(1 citation statement)

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“…To fill the abovementioned knowledge gap, a unique optical technique, digital holographic microscopy (DHM), allowing real-time observation of the 3D behavior of swimming bacteria, ,, was utilized to track the 3D motions of PAO1 during and after EP application. DHM has been used to study the impact of dead bacteria on the 3D movement of live bacteria and the influence of rough surfaces on human sperm migration. , It enables nonlabeled, high-throughput real-time 3D tracking of particles. , In the present study, cathodic EP was employed on a model water-borne microorganism, PAO1, which tends to adhere to environmental surfaces and form biofilms. The EP process lasted for several to tens of minutes, with an electric field intensity ( I e ) of less than 8.33 V cm –1 (2.5 V) .…”

Section: Introductionmentioning

confidence: 99%

Adaptive Escape of Pseudomonas aeruginosa by Application of Low-Amplitude Electric Pulses

Feng,

Liu,

Bao

et al. 2024

Langmuir

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Marine antibiofouling using low-amplitude electric pulses (EP) is an energy-efficient and eco-friendly approach, but potential mechanisms for preventing biofouling remain unclear. In the present study, the 3D adhesion dynamics of a model microorganism�Pseudomonas aeruginosa (PAO1)�under lowamplitude cathodic EP were examined as a function of applying voltage and its duration (t d ). The results demonstrated that adhered bacteria escaped from the electrode surface even when EP was removed. The escaped bacteria ratio, induction period of escape, and duration of the detachment were influenced profoundly by EP amplitude but slightly by t d when t d ≥ 5 min. The acceleration of escaped PAO1 from the surface indicated that their flagellar motor was powered by EP. Particularly, EP enabled swimming bacteria to have adaptive motions that were sustainable and regulated by the gene rsmA. As a result, they had less accumulation near the surface. The propulsion of adhered bacteria and adaptive escape of swimming bacteria were enhanced in response to low-amplitude EP. Hence, low-amplitude and short-duration EP is promising for sustainable antibiofouling applications.

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