Mechanism of action of colchicine

Chang, Yi‐Han

doi:10.1002/art.1780180511

Cited by 21 publications

(1 citation statement)

References 17 publications

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“…Many of its effects have been attributed to its interaction with a subunit protein of microtubules (9-11). Colchicine inhibits a variety of leukocyte functions (1 2) and may also exert a prime effect on the synoviocyte in the prophylaxis of acute gout (13).…”

Section: Discussionmentioning

confidence: 99%

Intravenous colchicine in the treatment of acute pseudogout

Tabatabai

Cummings

1980

Arthritis & Rheumatism

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Section: Discussionmentioning

confidence: 99%

Intravenous colchicine in the treatment of acute pseudogout

Tabatabai

Cummings

1980

Arthritis & Rheumatism

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Mechanisms Underlying the Suppression of Adjuvant‐Induced Arthritis by 6‐Mercaptopurine

Chang

1977

Arthritis & Rheumatism

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Daily oral administration of 6-mercaptopurine suppressed the development of the secondary (immune) lesions of adjuvant arthritis in a dose-related manner. The degrees of arthritis suppression corresponded closely to suppressions of concurrent development of the humoral and the cellular immune response to El, cells. A short course of therapy during the sensitization period (day -1 to day 5 when the day of adjuvant injection is designated as day 0) appeared to be almost as effective as continued daily dosing (day -1 to day 15). The drug did not influence the development of the primary (nonimmune) lesions of adjuvant arthritis at all dosage levels investigated. Chronic pretreatment with 10 mg/kg/day PO for 17 days had no effect on the development of carrageenin-induced acute inflammation in the rat. The suppression of adjuvant arthritis by 6-mercaptopurine, unlike suppression by cyclophosphamide, appears to result primarily from its suppressive action on the immune response.6-Mercaptopurine is known to have both immunosuppressive and antiinflammatory properties (1,2). The drug has been shown to suppress adjuvant-induced polyarthritis in the rat, a disease thought to be the result of a delayed hypersensitivity response to bacilli or their components (3,4), presumably by a combination of immunosuppressive and antiinflammatory actions (5,6). This study was undertaken to determine the relative contributions of antiinflammatory and immunosuppressive activities in the suppression of adjuvant arthritis by 6-mercaptopurine. MATERIALS AND METHODSMaintenance of El, Cell Line. The El, tumor cell line was a gift from Dr. J. Wunderlich, NIH, Bethesda, MD. El, cells were maintained by passage in syngeneic C57/bl 6 mice (Jackson Laboratories, Bar Harbor, ME). The cells (2 X lo7)were injected intraperitoneally on day 0. On day 7, approximately 3-5 X 108 cells per mouse were harvested from the peritoneum, and after washing with Hanks' minimum essential medium (HMEM), they were used either for replanting the cell line, for immunization, or as target cells in immune assays.Polyimmune Adjuvant Arthritis. Adjuvant arthritis was produced by a single subcutaneous injection of 1 mg of Mycobacterium butyricum (Difco Laboratories, Detroit, M I ) suspended in 0.1 ml of mineral oil into the right hind paw of male Lewis inbred rats (Microbiological Associates, Bethesda, MD) weighing between 235 and 250 g. A I .O ml suspension of El, cells ( 1 X 108 cells/ml) in HMEM was injected intraperitoneally immediately after the subplantar injection of adjuvant. The paw volume was measured by the method of Winter et al(7) on days 0,4, and 16 (or 22) with respect to the injection of adjuvant. All rats were treated with either saline or drug (PO or IM) daily from day -1 to day + 15 unless otherwise noted. After paw volume was measured on day 16, a sample of blood was taken and the spleen was removed. The serum and spleen cells were used to measure immune response.

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The effects of colchicine derivatives on humoral antibody response in mice

Trottier

Fitzgerald

1981

Drug Development Research

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The effects of colchicine and two of its chemical derivatives (propyl and bromo substitutions on the acetamido group) were investigated on the sheep red blood cell (SRBC) humoral antibody response in mice. These drugs were shown to suppress SRBC antibody titres. Immunosuppression was dependent upon temporal relationships of antigen/drug administration, as well as on the dosage profile of the drugs. In these studies bromocolchicine was the most active agent tested. Possible pharmacologic mechanisms are discussed.

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Mechanism of action of colchicine

Cited by 21 publications

References 17 publications

Intravenous colchicine in the treatment of acute pseudogout

Intravenous colchicine in the treatment of acute pseudogout

Mechanisms Underlying the Suppression of Adjuvant‐Induced Arthritis by 6‐Mercaptopurine

The effects of colchicine derivatives on humoral antibody response in mice

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