Mechanism of action of nitrification inhibitors based on dimethylpyrazole: A matter of chelation

Corrochano-Monsalve, Mario; González‐Murua, Carmen; Bozal-Leorri, Adrián; Lezama, Luís; Artetxe, Beñat

doi:10.1016/j.scitotenv.2020.141885

Cited by 44 publications

(23 citation statements)

References 68 publications

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“…Although DMPSA is not able to inhibit nitrification in pure cultures of N. europaea, its inhibition capacity in soil has been largely proven [19,20,22,[34][35][36][37][38]. Therefore, there must be a process that takes place in the soil, but does not occur in nitrifiers' pure culture that allows DMPSA inhibiting nitrification.…”

Section: Dmpsa Is Broken Into Dmp In Soilsmentioning

confidence: 99%

“…The active site of AMO contains Cu 2+ and thus, in general, nitrification is thought to be dependent of Cu 2+ availability [3,22,43]. In this sense, the action of nitrification inhibition of DMP-based NIs has been generally related to their presumed Cu 2+ chelating capacity [10].…”

Section: Copper Chelation Is Unlikely the Mode Of Action Of Dmp-based...mentioning

confidence: 99%

“…In this sense, the action of nitrification inhibition of DMP-based NIs has been generally related to their presumed Cu 2+ chelating capacity [10]. Corrochano-Monsalve et al [22] recently confirmed this ability for both DMPP and DMPSA through X-ray crystallography. These authors reported that the chelation efficiency of DMPP is 4 molecules of DMP per atom of Cu 2+ while for DMPSA the efficiency was 2 molecules of inhibitor per atom of Cu 2+ .…”

Section: Copper Chelation Is Unlikely the Mode Of Action Of Dmp-based...mentioning

confidence: 99%

“…In addition, they reported that four DMPP molecules are needed to chelate one atom of Cu 2+ , whilst DMPSA only needs two. In principle, this observation would make DMPSA a more efficient Cu 2+ chelator than DMPP [22]. However, it remains to be confirmed whether the Cu 2+ -chelating capacity of these compounds is responsible of their nitrification inhibition capacity.…”

mentioning

confidence: 99%

“…Ruser and Schulz [10] indicated that the mode of action of DMP-based NIs may be related to their attributed ability to chelate the Cu 2+ cations that ammonium monoxygenase (AMO) enzyme needs as co-factor [21]. Importantly, Corrochano-Monsalve et al [22] recently demonstrated that effectively both DMPP and DMPSA are able to chelate Cu 2+ . In addition, they reported that four DMPP molecules are needed to chelate one atom of Cu 2+ , whilst DMPSA only needs two.…”

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confidence: 99%

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Evidences towards deciphering the mode of action of dimethylpyrazole-based nitrification inhibitors in soil and pure cultures of Nitrosomonas europaea

Bozal-Leorri

Corrochano-Monsalve

Vega‐Mas

et al. 2022

Chem. Biol. Technol. Agric.

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Background Agriculture relies on the intensive use of synthetic nitrogen (N) fertilizers to maximize crop yields, which has led to the transformation of agricultural soils into high-nitrifying environments. Nevertheless, nitrification inhibitors (NIs) have been developed to suppress soil-nitrifier activity and decrease N losses. The NIs 3,4-dimethylpyrazole phosphate (DMPP) and 2-(3,4-dimethyl-1H-pyrazol-1-yl) succinic acid isomeric mixture (DMPSA) are able to reduce N2O emissions and maintain soil NH4+ for a longer time. Although both NIs have been proven to be effective to inhibit soil nitrification, their exact mode of action has not been confirmed. We aimed to provide novel insights to further understand the mode of action of DMP-based NIs. We evaluated the performance of DMPP and DMPSA in soil and pure cultures of nitrifying bacteria Nitrosomonas europaea. Results DMPSA did not inhibit nitrification in pure cultures of N. europaea. In the soil, we evidenced that DMPSA needs to be broken into DMP to achieve the inhibition of nitrification, which is mediated by a soil biological process that remains to be identified. Moreover, both DMPP and DMPSA are thought to inhibit nitrification due to their ability to chelate the Cu2+ cations that the ammonia monooxygenase enzyme (AMO) needs to carry on the first step of NH4+ oxidation. However, the efficiency of DMPP was not altered regardless the Cu2+ concentration in the medium. In addition, we also showed that DMPP targets AMO but not hydroxylamine oxidoreductase enzyme (HAO). Conclusions The inability of DMPSA to inhibit nitrification in pure cultures together with the high efficiency of DMPP to inhibit nitrification even in presence of toxic Cu2+ concentration in the medium, suggest that the mode of action of DMP-based NIs does not rely on their capacity as metal chelators. Graphical Abstract

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Section: Dmpsa Is Broken Into Dmp In Soilsmentioning

confidence: 99%

Section: Copper Chelation Is Unlikely the Mode Of Action Of Dmp-based...mentioning

confidence: 99%

Section: Copper Chelation Is Unlikely the Mode Of Action Of Dmp-based...mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Evidences towards deciphering the mode of action of dimethylpyrazole-based nitrification inhibitors in soil and pure cultures of Nitrosomonas europaea

Bozal-Leorri

Corrochano-Monsalve

Vega‐Mas

et al. 2022

Chem. Biol. Technol. Agric.

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The potential of 2,5‐dichloroaniline and ammonium thiosulphate and their combination as nitrification inhibitors

Mehmood,

Khadim,

Zaman

2023

Vietnam Journal of Chemistry

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In the current research, nitrification inhibitors such as 2,5‐dichloroaniline, ammonium thiosulphate, and their combination (1:1) were used to slow down the nitrification process to increase the nitrogen availability for crops. The various levels of 0.1, 0.25, and 0.5 % were used for each inhibitor on three types of soil. Leachates were collected from each treatment, add 1ml of 0.1 % chromotropic acid solution and 6 mL of concentrated sulfuric acid, swirled in the flask, and left at room temperature, yellow color was developed after 45 minutes. Then absorbance reading was noted for each sample by spectrophotometer at the wavelength of 430 nm. NO3‐ ‐N content was determined by the standard curve method. From inhibitors, the combination of both inhibitors shows minimum NO3‐ ‐N content as 154.29 and 112.83 ppm than other chemicals for 2nd and 14th days respectively. From soil, Sheikhupura soil shows minimum NO3‐ ‐N in the leachate as 176.26 (2nd day) and 105.03 ppm (14th day) as compared to other soils. From the levels, 0.25 % shows minimum NO3‐ ‐N as 118.05 (2nd day) and 63.18 ppm (14th day) as compared to other levels. The combination (1:1) and the level 0.25 % were found more suitable to reduce the nitrification process in Sheikhupura soil.

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Niche Differentiation Among Canonical Nitrifiers and N2O Reducers Is Linked to Varying Effects of Nitrification Inhibitors DCD and DMPP in Two Arable Soils

Fan

Chen

Yan³

et al. 2022

Microb Ecol

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Mechanism of action of nitrification inhibitors based on dimethylpyrazole: A matter of chelation

Cited by 44 publications

References 68 publications

Evidences towards deciphering the mode of action of dimethylpyrazole-based nitrification inhibitors in soil and pure cultures of Nitrosomonas europaea

Evidences towards deciphering the mode of action of dimethylpyrazole-based nitrification inhibitors in soil and pure cultures of Nitrosomonas europaea

The potential of 2,5‐dichloroaniline and ammonium thiosulphate and their combination as nitrification inhibitors

Niche Differentiation Among Canonical Nitrifiers and N2O Reducers Is Linked to Varying Effects of Nitrification Inhibitors DCD and DMPP in Two Arable Soils

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