Mechanism of Action of Potato Glycoalkaloids against Fusarium solani

Abstract

The antifungal mechanism of potato glycoalkaloids was studied using a sensitive species, Fusarium solani. The effects of potato glycoalkaloids extract on the ultrastructure, membrane permeability, contents of reducing sugar, soluble sugar, soluble protein and mycelial fat of Fusarium solani were determined. Potato glycoalkaloids significantly affected F. solani mycelial morphology, resulting in bubbly mycelial cell walls, incomplete outer layer, discontinuous cell membrane, disorganized structures of mitochondria and other organelles, and visible leakage of cell contents. Investigation of material metabolism indicated that potato glycoalkaloids disrupted selective permeability of mycelial cell membranes; In the treatment group, the soluble protein content increased from 66.50 g mL-1to 169.51 g mL-1for 0–6 h, the soluble sugar content in extracellular fluid increased from 117.4 g mL-1to 132.5 g mL-1for 0–24 h, which were much higher than that of the control group, hindered hydrolysis of reducing sugar, affected nutrient absorption and utilization and inhibited decomposition metabolism of mycelia. Thus, potato glycoalkaloids altered the morphology of fungal mycelia, destroyed cell membrane structure, increased mycelial cell membrane permeability, and caused cell contents leakage, resulting in effective inhibition of growth and metabolism of plant pathogenic fungus and so could decrease the occurrence of plant disease.© 2021 Friends Science Publishers

Effect of Potato Glycoside Alkaloids on Energy Metabolism of Fusarium solani

“…The green potato peels and buds were dried in a vacuum blast drying oven (80 °C, 12 h), ground using a grinder, and passed through an 80-mesh sieve. The obtained experimental samples were extracted by the acetic acid-ammonia precipitation method [17]; 100 g of the potato sample was mixed with 400 mL of 5% acetic acid, stirred for 60 min, and filtered. The residue was extracted twice with 200 mL of 5% acetic acid, the filtrate Mitochondria are the prominent organelles of cell energy metabolism and are an essential regulator of cell survival, signal transduction, differentiation, and cell cycle progression.…”

“…Structural damage or dysfunction of the mitochondria can significantly change the energy level of pathogenic fungi, affecting cell adaptability and leading to cell death [16]. Our previous studies have found that PGA treatment can inhibit the growth and development of F. solani by destroying its cell structure, interfering with substance metabolism, and inhibiting respiration and reactive oxygen species metabolism [17,18]. However, the effect of PGA on mitochondrial energy metabolism in F. solani is unclear.…”

Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani

“…Previous studies have shown that PGA can destroy the structure of mycelium by interfering with substance metabolism, inhibiting respiration and reducing ATP production ( He et al, 2021 ; Ding et al, 2023 ; Zhang et al, 2023 ). However, the effect of PGA on the TCA cycle of F. solani has not been reported, and its mechanism still needs to be clarified.…”