Mechanism of action of Sambucus williamsii Hance var. miquelii in the treatment of osteoporosis analyzed by UHPLC-HRMS/MS combined network pharmacology and experimental validation

Mu, Liting; Gao, Jinshuang; Zhang, Qingyu; Kong, Fanyu; Lian, Yiqi; Li, XiMeng; Ma, Zhen; Sun, Quan

doi:10.1016/j.fitote.2024.106003

Fitoterapia

2024

DOI: 10.1016/j.fitote.2024.106003

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Mechanism of action of Sambucus williamsii Hance var. miquelii in the treatment of osteoporosis analyzed by UHPLC-HRMS/MS combined network pharmacology and experimental validation

Liting Mu,

Jinshuang Gao,

Qingyu Zhang

et al.

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2024

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Cited by 2 publications

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Dissection of potential anti‐osteoporosis mechanism of isopsoralen – a quality control marker in Psoraleae Fructus – by metabolite profiling and network pharmacology

Ruan,

Wang,

Chen

et al. 2024

Rapid Comm Mass Spectrometry

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RationaleIsopsoralen (ISO), a quality control marker (Q‐marker) in Psoraleae Fructus, is proven to present an obvious anti‐osteoporosis effect. Until now, the metabolism and anti‐osteoporosis mechanisms of ISO have not been fully elucidated, greatly restricting its drug development.MethodsThe metabolites of ISO in rats were profiled by using ultrahigh‐performance liquid chromatography coupled with time‐of‐flight mass spectrometry. The potential anti‐osteoporosis mechanism of ISO in vivo was predicted by using network pharmacology.ResultsA total of 15 metabolites were characterized in rats after ingestion of ISO (20 mg/kg/day, by gavage), including 2 in plasma, 12 in urine, 6 in feces, 1 in heart, 3 in liver, 1 in spleen, 1 in lung, 3 in kidney, and 2 in brain. The pharmacology network results showed that ISO and its metabolites could regulate AKT1, SRC, NFKB1, EGFR, MAPK3, etc., involved in the prolactin signaling pathway, ErbB signaling pathway, thyroid hormone pathway, and PI3K‐Akt signaling pathway.ConclusionsThis is the first time for revealing the in vivo metabolism features and potential anti‐osteoporosis mechanism of ISO by metabolite profiling and network pharmacology, providing data for further verification of pharmacological mechanism.

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Dissection of potential anti‐osteoporosis mechanism of isopsoralen – a quality control marker in Psoraleae Fructus – by metabolite profiling and network pharmacology

Ruan,

Wang,

Chen

et al. 2024

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

Protective effects of arecanut seed phenols in retinoic acid induced osteoporosis and the potential mechanisms explored by network pharmacology

Tang,

Sun,

Song

et al. 2024

Front. Endocrinol.

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BackgroundArecanut seed is an important traditional medicine in Southeast Asia. It has been presented in a clinical formula to treat osteoporosis (OP) in China. Arecanut seed is abundant in phenols. However, most of current studies mainly focused on estrogen-deficient osteoporosis (OP) model of arecanut seed phenols (ASP), there is still a lack of roundly research about molecular mechanism of ASP therapy on OP and its influence on in drug-induced bone loss.Materials and methodsTo explore potential molecular mechanisms and the effects of ASP on OP, network pharmacology, molecular docking methods and a retinoic acid-induced OP rat model were employed in this study. According to the network pharmacology method, OP related targets and ASP compound related targets were collected from databases to obtain hub targets and top active chemicals in ASP treating OP. The potential therapic pathways were also calculated. Binding capacities of top active chemicals to hub targets were analyzed by molecular dock assay. In the animal experiment, osteocalcin (OCN) levels and alkaline phosphatase (ALP) activity in serum of all the rats were determined. The views of bone section were stained to observe the bone micro-structure of ASP affects. Bone mineral density (BMD), cortical bone thickness (CBT), area ratio of bone cortex (CAR) and area ratio of bone trabecula (TAR) were obtained from micro computed tomography to evaluate the effectiveness of ASP on bone loss.ConclusionThree hub genes and three top active compounds were screened by network pharmacology analysis and they combined well with each other. ASP had positive effects on alleviating RA-induced bone loss by regulating the expression of the hub genes. Signals in IL-17 pathway were predicted and primarily verified being potential targets in ASP treating OP.

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Mechanism of action of Sambucus williamsii Hance var. miquelii in the treatment of osteoporosis analyzed by UHPLC-HRMS/MS combined network pharmacology and experimental validation

Cited by 2 publications

References 12 publications

Dissection of potential anti‐osteoporosis mechanism of isopsoralen – a quality control marker in Psoraleae Fructus – by metabolite profiling and network pharmacology

Dissection of potential anti‐osteoporosis mechanism of isopsoralen – a quality control marker in Psoraleae Fructus – by metabolite profiling and network pharmacology

Protective effects of arecanut seed phenols in retinoic acid induced osteoporosis and the potential mechanisms explored by network pharmacology

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