Mechanism of Arabidopsis circ032768 Response to drought

Abstract

CircRNAs (CircularRNAs) reduce the abundance of mirnas (micrornas) in the way of competin gendogenousRNA, so as to regulate many physiological processes and stress responses in plants.However, little is known about the role of circRNA in drought stress.In this study, sequencing and biological analysis confirmed that circ032768, miR472 and RPS5 were responsive to drought stress, and the changes of gene expression were consistent with the prediction mechanism of ceRNA.The silencing vectors of circ032768 and STTM-miR472 were constructed by molecular biology techniques, and stable transgenic plants with drought tolerance were obtained.Further physiological and biochemical studies showed that circ032768 could bind to miR472, and miR472 could bind to RPS5 resistance gene, resulting in decreased expression of RPS5 gene.That is, circ032768 can competitively inhibit miR472 degradation of RPS5 in the manner of ceRNA.Thus, through the circ032768-miR472-RPS5 pathway, the RPS5 stress tolerance protein interacts with DREB2A protein to enhance the expression of downstream drought resistance genes RD29A and RD29B to participate in the drought resistance regulation mechanism of plants, so as to improve the drought tolerance of plants.