Mechanism of cell detachment from temperature-modulated, hydrophilic-hydrophobic polymer surfaces

Okano, Teruo; Yamada, Noriko; Okuhara, Minako; Sakai, Hideaki; Sakurai, Yasuhisa

doi:10.1016/0142-9612(95)93257-e

Cited by 840 publications

(570 citation statements)

References 21 publications

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“…The pioneering work by Okano et al [5] showed that cells could be released as intact monolayer cell sheets from the surface of PNIPAAm coated tissue culture polystyrene trays with their deposited ECM and preserved cell-cell and cell-ECM interactions. PNIPAAm switches its properties reversibly between hydrophobic (cell adhesive) and hydrophilic (non-cell adhesive) states at temperatures higher and lower than its lower critical solution temperature (LCST) (~32°C) respectively [5].…”

Section: Introductionmentioning

confidence: 99%

Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation

Chetty

Vargha

Maity

et al. 2013

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Growing cells on 3D scaffolds is far superior to the conventional 2D monolayer culture method. In this study, a novel 3D thermoresponsive poly(propylene-g-Nisopropylacrylamide) (PP-g-PNIPAAm) non-woven fabric (gNWF) was developed for cell culture using oxyfluorination-assisted graft polymerisation (OAGP). New polar functional groups were detected on the oNWF, and PNIPAAm was confirmed in the gNWF by Attentuated Total-Reflectance Fourier transform infrared (ATR-FTIR) and scanning x-ray photoelectron spectroscopy (S-XPS). Scanning electron microscopy (SEM) revealed a rough surface morphology and confinement of the PNIPAAm graft layer to the surface of the fibres in the gNWF. The OAGP method did not affect the crystalline phase of bulk PP, however, twin-melting thermal peaks were detected for the oNWF and gNWF indicating crystal defects. Contact angle studies showed that the surface of the gNWF exhibited a thermoresponsive behaviour. Hepatocyte cells attached onto gNWF disks in a bioreactor at 37ºC and remained viable for 10 days in culture. Upon cooling the cell culture media to 20ºC, cells were spontaneously released as 3D multi-cellular constructs without requiring destructive enzymes. The development of 3D thermoresponsive scaffolds capable of non-invasive 3D cell culture could provide a more reliable in vitro model for cells.

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Section: Introductionmentioning

confidence: 99%

Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation

Chetty

Vargha

Maity

et al. 2013

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…Previous efforts toward spatiotemporal control of tissue organization at the cellular scale have focused on modulation of the adhesive properties of the culture substrate (5)(6)(7). Through the micropatterning of surface chemistries that can be dynamically altered, localized attachment and release of cells has been demonstrated (8,9).…”

mentioning

confidence: 99%

Micromechanical control of cell–cell interactions

Hui

Bhatia

2007

Proc. Natl. Acad. Sci. U.S.A.

357

311

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The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By using a micromachined silicon substrate with moving parts, we demonstrate the dynamic regulation of cell-cell interactions via direct manipulation of adherent cells with micrometer-scale precision. We thereby achieve mechanical control of both tissue composition and spatial organization. As a case study, we demonstrate the utility of this tool in deconstructing the dynamics of intercellular communication between hepatocytes and supportive stromal cells in coculture. Our findings indicate that the maintenance of the hepatocellular phenotype by stroma requires direct contact for a limited time (Ϸhours) followed by a sustained soluble signal that has an effective range of <400 m. This platform enables investigation of dynamic cell-cell interaction in a multitude of applications, spanning embryogenesis, homeostasis, and pathogenic processes.dynamic substrate ͉ intercellular communication ͉ microelectromechanical systems ͉ microenvironment ͉ microfabrication

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“…Using electron irradiation, uniformly spread IPAAm monomers were polymerized and covalently immobilized onto the surface of the tissue culture polystyrene dish (TCPS) [21][22][23]. Although electron irradiation requires expensive equipment, this method facilitates large-scale production of thermoresponsive TCPS.…”

Section: Preparation and Characterization Of Pipaam-grafted Surfacesmentioning

confidence: 99%

“…Since 1990, our group has been developing the thermoresponsive cell culture dish grafted with PIPAAm on the surface, allowing cultured cells to be recovered by simply lowering the temperature below LCST (figure 2(a)) [21][22][23]. When the temperature is lowered, morphological changes to cells and the detachment of cells are suppressed by an ATP synthesis inhibitor, sodium azide, and a tyrosine kinase inhibitor, genistein [23,34].…”

Section: Thermoresponsive Cell Culture Dishmentioning

confidence: 99%

“…This alteration of the PIPAAm-modified intelligent surface can be used to modulate its interactions with solutes in the chromatographic separation [15][16][17][18][19][20] and with cultured cells [21][22][23]. PIPAAm-grafted chromatographic matrices allow us to regulate the retention of steroids [15,16], polypeptides and proteins [17,18], and nucleotides [19,20] by changing the temperature under the aqueous condition.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

Kobayashi¹,

Okano²

2010

Science and Technology of Advanced Materials

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This article reviews the properties and characterization of an intelligent thermoresponsive surface, which is a key technology for cell sheet-based tissue engineering. Intelligent thermoresponsive surfaces grafted with poly(N-isopropylacrylamide) exhibit hydrophilic/hydrophobic alteration in response to temperature change. Cultured cells are harvested on thermoresponsive cell culture dishes by decreasing the temperature without the use of digestive enzymes or chelating agents. Our group has developed cell sheet-based tissue engineering for therapeutic uses with single layer or multilayered cell sheets, which were recovered from the thermoresponsive cell culture dish. Using surface derivation techniques, we developed a new generation of thermoresponsive cell culture dishes to improve culture conditions. We also designed a new methodology for constructing well-defined organs using microfabrication techniques.

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Mechanism of cell detachment from temperature-modulated, hydrophilic-hydrophobic polymer surfaces

Cited by 840 publications

References 21 publications

Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation

Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation

Micromechanical control of cell–cell interactions

Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

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