Mechanism of ciprofloxacin resistance in Shigella dysenteriae

Lankeshwar, Nilima; Bagde, U. S.

doi:10.5897/ajb11.3952

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…In case of Azithromycin, zone reduced from 24 to 18 mm at their MIC after 13 subcultures. Nilima Lankeshwar et al [22] studies in 2013 and found that the S. dysenteriae resistant strain showed resistant to 10 µg/ml concentration of ciprofloxacin with no zone of inhibition, whereas in our study, ZOI reduced to 15 mm from 22 mm at their MIC (20 µg/ml) after development of resistance. From microscopic view it was observed that sensitive strain of Shigella dysenteriae was comparatively large in size and rod in shape, but the resistant strains look small and oval, instead.…”

Section: Changes In Genome Level Ofcontrasting

confidence: 61%

In-vitro Resistance Development by Ciprofloxacin and Azithromycin in Shigella dysenteriae

Islam

Haque

Sarker

et al. 2016

BMRJ

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Introduction: Drug resistance is a growing public health issue among the health care professionals. Due to improper use many bacterial species including the Shigella dysenteriae become resistant to the standard therapy. Objectives: In the present study, we have shown the bacterial resistance development in the Shigella dysenteriae species against Ciprofloxacin and Azithromycin and therefore decrease in their antibiotic susceptibility together with DNA and morphological alteration by the presence of sub inhibitory concentration of antibiotics. Methods: Disc diffusion method was applied to examine resistance of Shigella dysenteriae against ciprofloxacin and azithromycin and genomic anomalies were observed in ciprofloxacin resistant and sensitive strains by agarose gel electrophoresis. Results: Sub culturing in the ciprofloxacin reduced the ZOI from 22 to 15 mm at their MIC on 13 th passages but still the sensitivity was preserved in azithromycin (20 µg/dics) ZOI of 21 mm. In case Original Research Articleof Azithromycin, zone reduced from 24 to 18 mm at their MIC after 13 subcultures, but it raised high to 25 mm for both ciprofloxacin (20 µg/dics). As documented of genomic changes in ciprofloxacin resistant strain (Azithromycin not included) by agarose gel electrophoresis, six different DNA bands found whereas one DNA band found in ciprofloxacin sensitive strain. Conclusion: So it is clear that the misuse of existing antimicrobials may enable the microorganism to develop their less susceptible strain, by changing the restriction fragment length point which was appeared in the agarose gel, to that antimicrobial agent.

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Section: Changes In Genome Level Ofcontrasting

confidence: 61%

In-vitro Resistance Development by Ciprofloxacin and Azithromycin in Shigella dysenteriae

Islam

Haque

Sarker

et al. 2016

BMRJ

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Study on mechanism of cobalt resistance in Enterobacter species

M¹,

Bagde²

2019

IJMR

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Resistance by Enterobacter spp. towards several antimicrobial drugs and heavy metals: A review

M¹,

Bagde²

2017

Afr. J. Biotechnol.

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Antimicrobial resistance developed in several pathogens poses an increasing threat to human health across the world. No country can escape from the medical and economic impacts from this serious problem. Although the antibiotic resistance is not a new phenomenon, the current magnitude and speed with which it is developing is a cause for the global concern including in India. There are so many common diseases resulting from the microorganisms such as blood stream infections, urinary tract infections, post-operative wound infections and intra-abdominal infections. In this review the antimicrobial susceptibility or resistance of Enterobacter towards antimicrobial agents and heavy metals, viz. ceftazidime, moxifloxacin, nalidixic acid, sulfamethaxazole, and nickel and lead is discussed briefly along with other antimicrobials and heavy metals. The mechanisms behind the resistance by Enterobacter was analyzed and evaluated by many workers after using currently employed susceptibility testing methods for Enterobacter spp. There are some factors influencing mode of action of fluoroquinolones, quinolones and sulfamethaxazole. History, classification, identification, clinical features and treatment of infections and the epidemiology of antimicrobials (drugs and heavy metals) resistance by the Enterobacter spp. is included in this review. Now a day, antimicrobial resistance is common in hospitals where acquired infections can be perilous. This situation compels scientists to synthesize new antibiotics and treatment modalities. Enterobacter causes nosocomial infections. It is ubiquitous and can survive on skin and dry surfaces and replicate in contaminated fluids. Numerous outbreaks have been described. Various mechanisms have been adapted by microorganisms to resist toxicity of antimicrobials. Antimicrobial drugs may be rendered inactive or ineffective by the major ways such as barrier to antibiotic entry into the bacterial cell, prevention of the antibiotic from reaching the target, often by extrusion, alteration of the target of the drug and inactivation of the antibiotic by modification or destruction. In addition, bacteria may be able to bypass the metabolic pathway affected by a particular drug or may be able to overproduce an enzyme that is inhibited by the drug action, more than one mechanism may operate at any given time

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Mechanism of ciprofloxacin resistance in Shigella dysenteriae

Cited by 4 publications

References 17 publications

In-vitro Resistance Development by Ciprofloxacin and Azithromycin in Shigella dysenteriae

In-vitro Resistance Development by Ciprofloxacin and Azithromycin in Shigella dysenteriae

Study on mechanism of cobalt resistance in Enterobacter species

Resistance by Enterobacter spp. towards several antimicrobial drugs and heavy metals: A review

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