Mechanism of dexamethasone suppression of brain tumor-associated vascular permeability in rats. Involvement of the glucocorticoid receptor and vascular permeability factor.

Heiss, John D.; Papavassiliou, Efstathios; Merrill, Marsha J.; Nieman, Lynnette K.; Knightly, J J; Walbridge, Stuart; Edwards, Nancy A.; Oldfield, Edward H.

doi:10.1172/jci118927

Cited by 241 publications

(154 citation statements)

References 65 publications

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“…In this study cells secreted VEGF in all conditions and although VEGF was slightly inhibited by Dex this recovered at later time‐points. Work has also indicated that Dex may inhibit VEGF (Heiss et al, 1996). The rising levels of VEGF indicate that if implanted, the cells in the scaffolds would support recruitment of endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds

Puwanun

Delaine-Smith

Colley

et al. 2017

J Tissue Eng Regen Med

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Biodegradable electrospun polycaprolactone scaffolds can be used to support bone‐forming cells and could fill a thin bony defect, such as in cleft palate. Oscillatory fluid flow has been shown to stimulate bone production in human progenitor cells in monolayer culture. The aim of this study was to examine whether bone matrix production by primary human mesenchymal stem cells from bone marrow or jaw periosteal tissue could be stimulated using oscillatory fluid flow supplied by a standard see‐saw rocker. This was investigated for cells in two‐dimensional culture and within electrospun polycaprolactone scaffolds. From day 4 of culture onwards, samples were rocked at 45 cycles/min for 1 h/day, 5 days/week (rocking group). Cell viability, calcium deposition, collagen production, alkaline phosphatase activity and vascular endothelial growth factor secretion were evaluated to assess the ability of the cells to undergo bone differentiation and induce vascularisation. Both cell types produced more mineralized tissue when subjected to rocking and supplemented with dexamethasone. Mesenchymal progenitors and primary human mesenchymal stem cells from bone marrow in three‐dimensional scaffolds upregulated mineral deposition after rocking culture as assessed by micro‐computed tomography and alizarin red staining. Interestingly, vascular endothelial growth factor secretion, which has previously been shown to be mechanically sensitive, was not altered by rocking in this system and was inhibited by dexamethasone. Rocker culture may be a cost effective, simple pretreatment for bone tissue engineering for small defects such as cleft palate.

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Section: Discussionmentioning

confidence: 99%

A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds

Puwanun

Delaine-Smith

Colley

et al. 2017

J Tissue Eng Regen Med

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“…Wheals (5 mm in diameter) corresponding to the site of sample injection were individually resected and incubated in 100 AL of formamide at 37jC for 48 h to extract Evans blue dye. The amount of Evans blue dye was determined by measuring the absorbance at 660 nm in a spectrophotometer (31).…”

Section: Targeted Therapy Of Orthotopic Human Lung Cancermentioning

confidence: 99%

Targeted therapy of orthotopic human lung cancer by combined vascular endothelial growth factor and epidermal growth factor receptor signaling blockade

Wu¹,

Onn²,

Isobe³

et al. 2007

Molecular Cancer Therapeutics

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The outcome for patients with lung cancer has not changed significantly for more than two decades. Several studies show that the overexpression of vascular endothelial growth factor (VEGF)/vascular permeability factor and epidermal growth factor (EGF) and their receptors correlates with the clinical outcome for lung cancer patients. However, clinical trials of agents that target either of these pathways alone have been disappointing. We hypothesize that targeting both the tumor and its vasculature by simultaneously blocking the VEGFR and EGFR pathways will improve the treatment of locoregional lung cancer. Human lung cancer specimens were first examined for the activation of VEGF receptor 2 (VEGFR2) and EGF receptor (EGFR) for tumor and tumor-associated endothelial cells, and both were found to be activated. The effects of ZD6474 (ZACTIMA), a small-molecule inhibitor of VEGFR2 and EGFR tyrosine kinases, were then studied in vitro using human lung cancer and microvascular endothelial cells. In vitro, ZD6474 inhibited EGFR, VEGFR2, mitogenactivated protein kinase and Akt phosphorylation, EGF-and VEGF-induced proliferation, and endothelial cell tube formation and also induced apoptosis. ZD6474 was further studied in vivo using an orthotopic mouse model of nonsmall cell lung cancer using NCI-H441 human lung adenocarcinoma cells. The inhibition of both VEGFR2 and EGFR signaling pathways by ZD6474 resulted in profound antiangiogenic, antivascular, and antitumor effects. These results provide a basis for the development of clinical strategies for the combination of selective protein tyrosine kinase inhibitors that block both EGFR and VEGFR signaling as part of the management of locally advanced lung cancer. [Mol Cancer Ther 2007;6(2):471 -83]

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“…The role of dexamethasone in reducing peritumoural blood brain barrier permeability and inhibition of vascular endothelial growth factor action is well reported in the literature. 13 …”

Section: Discussionmentioning

confidence: 99%

Silent meningioma: a cause of quadriperesis in total knee arthroplasty

Suresh

Chandrashekar²,

Nataraj

2017

Int J Res Orthop

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Total knee arthroplasty (TKA) is a treatment for end stage osteoarthritis. Majority of them in the older age group with associated co-morbidities and increased the risk for stroke. Asymptomatic meningiomas may become symptomatic and presents with variable symptoms depending on the size and location of the tumour. We report a case of a 58 years old lady who underwent bilateral TKA under combined spinal epidural anaesthesia with an uneventful post-operative course with post-operative analgesia. Patient developed dysphasia and weakness in upper and lower limbs. Following computed tomography of the brain revealed a meningioma in the falx region. Patient treated with steroid and underwent elective surgery for meningioma without any complications. Asymptomatic brain tumours can become symptomatic after surgery and urgent initiation of neurologic evaluation and strong recommendation to initiate intracranial pressure management oriented therapy in time.

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Mechanism of dexamethasone suppression of brain tumor-associated vascular permeability in rats. Involvement of the glucocorticoid receptor and vascular permeability factor.

Cited by 241 publications

References 65 publications

A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds

A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds

Targeted therapy of orthotopic human lung cancer by combined vascular endothelial growth factor and epidermal growth factor receptor signaling blockade

Silent meningioma: a cause of quadriperesis in total knee arthroplasty

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