Mechanism of erythromycin-induced ermC mRNA stability in Bacillus subtilis

Bechhofer, David H.; Zen, Keika

doi:10.1128/jb.171.11.5803-5811.1989

Cited by 73 publications

(70 citation statements)

References 52 publications

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“…In both S. aureus and B. subtilis, the stalled ribosome induces methyltransferase synthesis by disrupting base-pairing that would otherwise occlude the RBS; in addition, it prolongs the lifetime of ermA and ermC mRNA up to 20-fold by a mechanism independent of the increase in translation (Bechhofer and Dubnau 1987;Sandler and Weisblum 1988). Interestingly, when the 5Ј end of either erm transcript is extended by fusing additional RNA there, erythromycin-induced ribosome stalling protects only the mRNA segment downstream of the stall site, consistent with a directional stabilization mechanism involving 5Ј-end blockade when a ribosome is stalled nearby (Bechhofer and Zen 1989;Sandler and Weisblum 1989). A similar mechanism has been invoked to explain how the S. aureus cat (chloramphenicol acetyltransferase) transcript is stabilized by subinhibitory concentrations of chloramphenicol (Dreher and Matzura 1991).…”

Section: Stabilization By Stalled Ribosomesmentioning

confidence: 64%

Lost in translation: the influence of ribosomes on bacterial mRNA decay: Figure 1.

Deana

Belasco²

2005

Genes Dev.

279

252

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The lifetimes of bacterial mRNAs are strongly affected by their association with ribosomes. Events occurring at any stage during translation, including ribosome binding, polypeptide elongation, or translation termination, can influence the susceptibility of mRNA to ribonuclease attack. Ribosomes usually act as protective barriers that impede mRNA cleavage, but in some instances they can instead trigger the decay of the mRNA to which they are bound or send a signal that leads to widespread mRNA destabilization within a cell. The influence of translation on mRNA decay provides a quality-control mechanism for minimizing the use of poorly or improperly translated mRNAs as templates for the production of abnormal proteins that might be toxic to bacteria.

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Section: Stabilization By Stalled Ribosomesmentioning

confidence: 64%

Lost in translation: the influence of ribosomes on bacterial mRNA decay: Figure 1.

Deana

Belasco²

2005

Genes Dev.

279

252

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“…The half-life of the ermC transcript increases about 20-fold upon exposure of the bacteria to erythromycin (Bechhofer & Zen, 1989). Binding of the drug to ribosomes translating a short ORF preceding the coding region for the ErmC protein stalls the ribosomes at this ORF.…”

Section: Discussionmentioning

confidence: 99%

The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis

2000

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The Bacillus subtilis aprE gene encodes subtilisin, an extracellular proteolytic enzyme produced in stationary phase. The authors examined the stability of aprE mRNA and aprE leader-lacZ fusion mRNA. Both mRNAs were found to be unusually stable, with half-lives longer than 25 min, demonstrating that the aprE leader contains a determinant for extreme mRNA stability. The half-lives were the same in growing and stationary-phase cells. This contrasts with the findings of O. Resnekov et al. (1990) [Proc Natl Acad Sci U S A 87, 8355-8359], which suggested a growth-phase-dependent mechanism for decay of aprE mRNA. The discrepancy is explained by the techniques used. Substitution of two bases or deletion of 25 nucleotides in the aprE leader led to a major difference in its predicted secondary structure and resulted in a fivefold reduction of the half-life of aprE mRNA. The authors also determined the halflife of amyE mRNA, which encodes α-amylase, another stationary-phase, excreted enzyme and found it to be around 5 min. This shows that extreme stability is not a general property of stationary-phase mRNAs encoding excreted enzymes.

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“…The question of whether the proximity of the stalling sequence (codons 5 to 9) to the 5Ј end of the erm RNA (Ͻ10 nt upstream of the ribosome binding site) is important for the on May 11, 2018 by guest http://mmbr.asm.org/ stabilizing effect was addressed by cloning heterologous extensions between the promoter and the leader peptide for both ermA (204) and ermC (16). In both cases, a full-length unstable RNA and a shorter stable species were detected in the presence of erythromycin.…”

Section: Stability Determinants Of Mrnasmentioning

confidence: 99%

RNA Processing and Degradation inBacillus subtilis

Condon

2003

Microbiol Mol Biol Rev

141

134

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This review focuses on the enzymes and pathways of RNA processing and degradation in Bacillus subtilis, and compares them to those of its gram-negative counterpart, Escherichia coli. A comparison of the genomes from the two organisms reveals that B. subtilis has a very different selection of RNases available for RNA maturation. Of 17 characterized ribonuclease activities thus far identified in E. coli and B. subtilis, only 6 are shared, 3 exoribonucleases and 3 endoribonucleases. Some enzymes essential for cell viability in E. coli, such as RNase E and oligoribonuclease, do not have homologs in B. subtilis, and of those enzymes in common, some combinations are essential in one organism but not in the other. The degradation pathways and transcript half-lives have been examined to various degrees for a dozen or so B. subtilis mRNAs. The determinants of mRNA stability have been characterized for a number of these and point to a fundamentally different process in the initiation of mRNA decay. While RNase E binds to the 5′ end and catalyzes the rate-limiting cleavage of the majority of E. coli RNAs by looping to internal sites, the equivalent nuclease in B. subtilis, although not yet identified, is predicted to scan or track from the 5′ end. RNase E can also access cleavage sites directly, albeit less efficiently, while the enzyme responsible for initiating the decay of B. subtilis mRNAs appears incapable of direct entry. Thus, unlike E. coli, RNAs possessing stable secondary structures or sites for protein or ribosome binding near the 5′ end can have very long half-lives even if the RNA is not protected by translation

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Mechanism of erythromycin-induced ermC mRNA stability in Bacillus subtilis

Cited by 73 publications

References 52 publications

Lost in translation: the influence of ribosomes on bacterial mRNA decay: Figure 1.

Lost in translation: the influence of ribosomes on bacterial mRNA decay: Figure 1.

The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis

RNA Processing and Degradation inBacillus subtilis

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