Mechanism of formaldehyde biodegradation by Pseudomonas putida

Adroer, Núria; Casas, C.; Mas, Carles de; Solá, Carles Miralles

doi:10.1007/bf00176528

Cited by 70 publications

(40 citation statements)

References 10 publications

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“…Ethylene glycol, commonly used as a scale inhibitor in hydraulic fracturing fluids (Gregory et al 2011), is readily biodegradable across a range of conditions in aqueous and soil environments (Evans and David 1974;Klecka et al 1993;Mrklas et al 2004), generally reaching full removal over short timespans (Staples et al 2001). Similarly, isopropanol, which is used as a surfactant, and common biocides formaldehyde and glutaraldehyde can be easily degraded by aerobic and anaerobic microbial consortia (Adroer et al 1990;Bustard et al 2000;Leung 2001;Kahrilas et al 2015). However, when not the sole substrate for microbial growth, the biodegradation efficiency of these and other additives may be dramatically reduced.…”

Section: Introductionmentioning

confidence: 97%

Aerobic biodegradation of organic compounds in hydraulic fracturing fluids

et al. 2015

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Little is known of the attenuation of chemical mixtures created for hydraulic fracturing within the natural environment. A synthetic hydraulic fracturing fluid was developed from disclosed industry formulas and produced for laboratory experiments using commercial additives in use by Marcellus shale field crews. The experiments employed an internationally accepted standard method (OECD 301A) to evaluate aerobic biodegradation potential of the fluid mixture by monitoring the removal of dissolved organic carbon (DOC) from an aqueous solution by activated sludge and lake water microbial consortia for two substrate concentrations and four salinities. Microbial degradation removed from 57 % to more than 90 % of added DOC within 6.5 days, with higher removal efficiency at more dilute concentrations and little difference in overall removal extent between sludge and lake microbe treatments. The alcohols isopropanol and octanol were degraded to levels below detection limits while the solvent acetone accumulated in biological treatments through time. Salinity concentrations of 40 g/L or more completely inhibited degradation during the first 6.5 days of incubation with the synthetic hydraulic fracturing fluid even though communities were pre-acclimated to salt. Initially diverse microbial communities became dominated by 16S rRNA sequences affiliated with Pseudomonas and other Pseudomonadaceae after incubation with the synthetic fracturing fluid, taxa which may be involved in acetone production. These data expand our understanding of constraints on the biodegradation potential of organic compounds in hydraulic fracturing fluids under aerobic conditions in the event that they are accidentally released to surface waters and shallow soils.

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Section: Introductionmentioning

confidence: 97%

Aerobic biodegradation of organic compounds in hydraulic fracturing fluids

et al. 2015

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“…The enzyme formaldehyde dismutase has been found in some species of Pseudomonas. [17][18][19][20][21][22] In order to verify our hypothesis of a reverse dismutase reaction, the presence of a dismutase had to be proved. In the presence of oxygen, Fal is rapidly oxidized to formate and further to CO2.…”

Section: Resultsmentioning

confidence: 99%

Formate-stimulated Oxidation of Methanol byPseudomonas putida9816

Riis

Miethe

Babel

2003

Bioscience, Biotechnology, and Biochemistry

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“…Mineral salts medium enriched with 5 g of glucose/l (MSM + G) and complex medium enriched with 5 g of glucose/l (CM + G) were used to study biodegradation of formaldehyde in the presence of both formaldehyde and glucose (Adroer et al 1990;Azachi et al 1995;Doronina et al 1997).…”

Section: Isolation Of Bacteriamentioning

confidence: 99%

“…Formaldehyde (H-CHO) is a compound extensively used in the chemical manufacturing industries, and is frequently released or escapes into the environment, causing environmental pollution (Adroer et al 1990). It is widely used in medicine, agriculture and industry.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Bacteria Able to Metabolize High Concentrations of Formaldehyde

Mirdamadi

Rajabi

Khalilzadeh

et al. 2005

World J Microbiol Biotechnol

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Nineteen bacterial strains able to degrade and metabolize formaldehyde as a sole carbon source were isolated from soil and wastewater of a formaldehyde production factory. The samples were cultured in complex and mineral salts media containing 370 mg formaldehyde/l. The bacterial strains were identified to be Pseudomonas pseudoalcaligenes, P. aeruginosa, P. testosteroni, P. putida, and Methylobacterium extorquens. After adaptation of these microorganisms to high concentrations of formaldehyde; two isolated strains of M. extorquens (strains ESS and PSS) and four strains of P. pseudoalcaligenes (strains LSW, SSW, NSW and OSS) degraded 1850 mg formaldehyde/l, where as P. pseudoalcaligenes strain OSS completely consumed 3700 mg of formaldehyde/l after 24 h and degraded 70% of 5920 mg of formaldehyde/l after 72 h.

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Mechanism of formaldehyde biodegradation by Pseudomonas putida

Cited by 70 publications

References 10 publications

Aerobic biodegradation of organic compounds in hydraulic fracturing fluids

Aerobic biodegradation of organic compounds in hydraulic fracturing fluids

Formate-stimulated Oxidation of Methanol byPseudomonas putida9816

Isolation of Bacteria Able to Metabolize High Concentrations of Formaldehyde

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