Mechanism of Free Zn(2+) Enhancing Inhibitory Effects of EGCG on the Growth of PC-3 Cells: Interactions with Mitochondria

Yang, Jing; Yu, Haining; Sun, Shili; Zhang, Lancui; Das, Undurti N.; Ruan, Hui; He, Guoqing; Shen, Shengrong

doi:10.1007/s12011-009-8362-5

Cited by 21 publications

(17 citation statements)

References 44 publications

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“…EGCG associated with heavy metals (eg, Zn 2+ and Cd 2+ ) has reportedly been able to enhance the biological activities of EGCG,41,42 particularly in tumor cell necrosis or apoptosis 41. Shukla et al43 reported that the development of radioactive gold nanoparticles combined with EGCG ( 198 AuNP-EGCG) effectively uploaded nanoparticles onto prostate tumor cells and facilitated EGCG internalization through 67 laminin receptors.…”

Section: Resultsmentioning

confidence: 99%

Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells

Chen¹,

Hsieh²,

Huang³

et al. 2014

DDDT

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(–)-Epigallocatechin-3-gallate (EGCG), the major bioactive constituent in green tea, has been reported to effectively inhibit the formation and development of tumors. To maximize the effectiveness of EGCG, we attached it to nanogold particles (EGCG-pNG) in various ratios to examine in vitro cytotoxicity and in vivo anti-cancer activity. EGCG-pNG showed improved anti-cancer efficacy in B16F10 murine melanoma cells; the cytotoxic effect in the melanoma cells treated with EGCG-pNG was 4.91 times higher than those treated with EGCG. The enhancement is achieved through mitochondrial pathway-mediated apoptosis as determined by annexin V assay, JC-10 staining, and caspase-3, -8, -9 activity assay. Moreover, EGCG-pNG was 1.66 times more potent than EGCG for inhibition of tumor growth in a murine melanoma model. In the hemolysis assay, the pNG surface conjugated with EGCG is most likely the key factor that contributes to the decreased release of hemoglobin from human red blood cells.

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Section: Resultsmentioning

confidence: 99%

Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells

Chen¹,

Hsieh²,

Huang³

et al. 2014

DDDT

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show abstract

“…Experimental data have shown that in PCa cell lines, a mixture of Zn 2+ and EGCG enhances both the growth inhibitory effect and the free radical scavenging ability with respect to EGCG alone. Moreover, Zn 2+ enhances EGCG effects in terms of reduction of mitochondrial membrane potential and caspase-9 activation [36][37][38]. In PC-3 ML and in IBC-10a primary prostate cell lines, the administration of EGCG (up to 60 μM) determines a reduction of mitochondrial membrane potential and integrity, followed by apoptosis without increase in ROS production [39].…”

Section: Effects Of Gtcs On Intracellular Ros Productionmentioning

confidence: 99%

Green Tea Catechins for Prostate Cancer Prevention: Present Achievements and Future Challenges

Naponelli¹,

Ramazzina²,

Lenzi³

et al. 2017

Preprint

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Green Tea Catechins (GTCs) are a family of chemically related compounds usually classified as antioxidant molecules. Epidemiological evidences, supported by interventional studies, highlighted a more than promising role for GTCs in human Prostate Cancer (PCa) chemoprevention.In the last decades many efforts have been made to gain new insights into the mechanism of action of GTCs. Now it is clear that GTCs anticancer action can no longer be simplistically limited to their direct antioxidant/pro-oxidant properties. Recent contributions to the advancement of knowledge in this field have shown that GTCs specifically interact with cellular targets including, cell surface receptors, lipid rafts and endoplasmic reticulum, modulate gene expression through direct effect on transcription factors or indirect epigenetic mechanisms, interfere with intracellular proteostasis at various levels. Many of the effects observed in vitro are dose and cell context dependent and take place at concentration that cannot be achieved in vivo.Poor intestinal absorption together with an extensive systemic and enteric metabolism influence GTCs bioavailability through still poor understood mechanisms. Recent efforts to develop delivery systems that increase GTCs overall bioavailability, by mean of biopolymeric nanoparticles, represent the main way to translate preclinical results in a real clinical scenario for PCa chemoprevention.

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“…Experimental data have shown that, in PCa cell lines, a mixture of Zn 2+ and EGCG enhances both the growth inhibitory effect and the free radical scavenging ability with respect to EGCG alone. Moreover, Zn 2+ enhances EGCG effects in terms of reduction of mitochondrial membrane potential and caspase-9 activation [36,37,38]. In PC-3 ML and in IBC-10a primary prostate cell lines, the administration of EGCG (up to 60 µM) determines a reduction of mitochondrial membrane potential and integrity, followed by apoptosis without increase in ROS production [39].…”

Section: Effects Of Gtcs On Cellular Redox Homeostasis and Antioximentioning

confidence: 99%

Green Tea Catechins for Prostate Cancer Prevention: Present Achievements and Future Challenges

et al. 2017

View full text Add to dashboard Cite

Green tea catechins (GTCs) are a family of chemically related compounds usually classified as antioxidant molecules. Epidemiological evidences, supported by interventional studies, highlighted a more than promising role for GTCs in human prostate cancer (PCa) chemoprevention. In the last decades, many efforts have been made to gain new insights into the mechanism of action of GTCs. Now it is clear that GTCs’ anticancer action can no longer be simplistically limited to their direct antioxidant/pro-oxidant properties. Recent contributions to the advancement of knowledge in this field have shown that GTCs specifically interact with cellular targets, including cell surface receptors, lipid rafts, and endoplasmic reticulum, modulate gene expression through direct effect on transcription factors or indirect epigenetic mechanisms, and interfere with intracellular proteostasis at various levels. Many of the effects observed in vitro are dose and cell context dependent and take place at concentrations that cannot be achieved in vivo. Poor intestinal absorption together with an extensive systemic and enteric metabolism influence GTCs’ bioavailability through still poorly understood mechanisms. Recent efforts to develop delivery systems that increase GTCs’ overall bioavailability, by means of biopolymeric nanoparticles, represent the main way to translate preclinical results in a real clinical scenario for PCa chemoprevention.

show abstract

Mechanism of Free Zn(2+) Enhancing Inhibitory Effects of EGCG on the Growth of PC-3 Cells: Interactions with Mitochondria

Cited by 21 publications

References 44 publications

Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells

Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells

Green Tea Catechins for Prostate Cancer Prevention: Present Achievements and Future Challenges

Green Tea Catechins for Prostate Cancer Prevention: Present Achievements and Future Challenges

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