Mechanism of Histaminase Liberation in Guinea Pig Anaphylaxis

Giertz, H.; Hahn, F.

doi:10.1159/000230719

Cited by 12 publications

(5 citation statements)

References 3 publications

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“…The heparin-induced rise in plasma histaminase (diamine oxidase) activity has been observed in man (5,6), guinea pig (22,23), rabbit (23), rat (23), and other vertebrate species (24). There is evidence that this rise is due to release of enzyme from liver in guinea pig (22) and intestine in rat (25).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Response of Plasma Histaminase Activity to Small Doses of Heparin in Normal Subjects and Patients with Hyperlipoproteinemia

Baylin¹,

Beaven²,

Krauss³

et al. 1973

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 99%

“…There is evidence that this rise is due to release of enzyme from liver in guinea pig (22) and intestine in rat (25). The source of the enzyme in man is not known, but because human kidney and intestine contain appreciable histaminase activity (12) (5,6).…”

Section: Discussionmentioning

confidence: 99%

Response of Plasma Histaminase Activity to Small Doses of Heparin in Normal Subjects and Patients with Hyperlipoproteinemia

Baylin¹,

Beaven²,

Krauss³

et al. 1973

J. Clin. Invest.

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“…DAO activity is produced in particularly high amounts by the maternal placenta (5,6), and this results in large increases in enzyme activity in plasma (e.g., [7][8][9][10][11] (unpublished data) during pregnancy. In nonpregnant animals, the enzyme is released upon injection of heparin into the circulation of humans (12)(13)(14) and the rat, rabbit (15), and guinea pig (15,16). Inasmuch as this release is apparent within 20 s (14) and because DAO activity is present in or on microvascular endothelial cells from rat and guinea pig (17), we have examined the possibility that heparin might act by displacement of DAO from vascular endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Binding of diamine oxidase activity to rat and guinea pig microvascular endothelial cells. Comparisons with lipoprotein lipase binding.

Robinson‐White¹,

Baylin²,

Olivecrona³

et al. 1985

J. Clin. Invest.

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Microvascular endothelial cells from rat and guinea pig fat pads were shown to bind diamine oxidase (DAO) activity when incubated with soluble extracts of placenta (33 DAO U/mg of placenta) and a purified placental enzyme preparation (94 U/ gg of protein). The extent of binding was dependent on the concentration of enzyme activity and tissue. Saturation of binding sites with 5,000 U of DAO/ml resulted in levels of bound activity (up to 11-13 U/mg of endothelial cells) in excess of that observed in all tissues except placenta. Scatchard plots suggested that there were at least two DAO binding sites (apparent K., 92 and 2,450 U/ml). Although the same cell preparations bound 125I-labeled lipoprotein lipase (LPL), the presence of LPL on the endothelial cell surface did not interfere with the binding of DAO activity except when cells were exposed to high concentrations of LPL. Alternatively, bound DAO activity was partially displaced (up to 33%) only with high concentrations (30 zg/ml) of LPL. DAO activity may thus be bound to at least two populations of sites, one of which may bind LPL. Both enzymes, however, were displaced by heparin (0.05-5 U/ml) and DAO binding was impaired by prior treatment of cells with proteolytic and glycosaminoglycandegrading enzymes. The demonstration of DAO binding to vascular endothelial cells provides a further example of the ability of these cells to bind enzymes at their surface and thereby act on biologically active substances in the circulation.

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“…On the other hand, as in guinea pigs [11], a correlation of histaminase and heparin seems to exist in 'early' and 'late' anaphylaxis of the rat. The fact that heparin levels and histaminase activity are both higher in 'late' than in 'early' anaphylaxis suggests that heparin may be the mediator of histaminaseliberation in rats as observed in guinea pigs [3]. This assumption is further supported by recent observations that heparin has a histaminase liberating effect also in rats [7,9].…”

Section: Discussionmentioning

confidence: 69%