Mechanism of Inhibition of CYP1A1 and Glutathione S-Transferase Activities in Fish Liver by Quercetin, Resveratrol, Naringenin, Hesperidin, and Rutin

Abstract

Quercetin, resveratrol, naringenin, hesperidin, and rutin are phenolic compounds/flavonoids that may have roles in the reduction of cancer susceptibility. In this study, in vitro modulatory effects of them were studied on liver CYP1A1 associated 7-ethoxyresorufin-O-deethylase (EROD) activity and glutathione S-transferase (GST) activity of leaping mullet (Liza saliens). All of the phenolic compounds/flavonoids used exerted an inhibitory effect on both EROD and GST activities of fish. Quercetin, resveratrol, hesperidin, and rutin were found to inhibit EROD activity in a competitive manner; on the other hand, naringenin was found to inhibit EROD activity in a noncompetitive manner. Ki values of quercetin, resveratrol, naringenin, hesperidin, and rutin were calculated from Dixon plots as 0.12 μM, 0.67 μM, 2.63 μM, 18 μM and 0.1 mM, respectively. Resveratrol, quercetin, and hesperidin were found to inhibit GST activity in a competitive manner; on the other hand, rutin and naringenin were found to inhibit GST activity in a mixed-type manner. Ki values of resveratrol, quercetin, hesperidin, naringenin, and rutin were calculated from Dixon plots as 3.2 μM, 12.5 μM, 45 μM, 128 μM, and 150 μM, respectively. The results suggest that quercetin and resveratrol containing foods are effective in the prevention and treatment of cancer.

Chemical Constituents, Quantitative Analysis and Antioxidant Activities ofEchinacea purpurea(L.) Moench andEchinacea pallida(Nutt.) Nutt.

“…Rutin, a pharmaceutically and medicinally valuable compound, existed in methanol and water extracts of both plant species. Recent work about the rutin indicated that it has antioxidant (Das et al 2015), antibacterial (Ananth et al 2015), antidiabetic (Wang et al 2015), anticancer (Arinc et al 2015), antiepileptic (Dubey et al 2015), antimalarial (Choudhary et al 2014) and antithyroid (Panda and Kar 2014) properties. The water extract of E. purpurea leaves, E. purpurea flowers, E. pallida leaves and E. pallida flowers revealed the excellent ABTS cation radical (Fig.…”

LC-MS Analysis of Phytocomponents in the Methanol Extract of Piper Sarmentosum Leaves

“…40 Hesperidin and rutin are vast and ranging in its ability to inhibit liver enzymes due to their antithyroid effects. 41,42 It has also been shown to possess anti-inflammatoty, antioxidant, hypolipaedemic and anti-carcinogenic activities. 43 Pipentol is a known flavouring agent which are commoly used in fragrance chemistry.…”

Modulation of mRNA expression and activities of xenobiotic metabolizing enzymes, CYP1A1, CYP1A2, CYP2E1, GPx and GSTP1 by the Salicornia freitagii extract in HT-29 human colon cancer cells

“…Fold-inhibition was calculated using the following formula: 2 -∆∆Ct , where ∆∆Ct=∆Ct(treated)−∆Ct(control); ∆Ct(treated)=∆Ct(CY Ps)−∆Ct(GAPDH); ∆Ct(control)=∆Ct(CYPs)−∆Ct(GAPDH). especially by preventing the induction of CYP1A isozymes [42,43]. In light of the role of CYP1A in the formation of reactive products, the observed inhibition of CYP1A mRNA expression by the SF extract and its major phenolic component vanillic acid could lead to the reduction or prevention of the onset of many diseases, including cancer.…”