Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

Hou, Yu; Feng, Helin; Jiao, Jianbao; Qian, Ligang; Sun, Bo; Chen, Pingtao; Li, Qinggui; Liang, Zhixing

doi:10.1080/21691401.2019.1620252

Cited by 23 publications

(18 citation statements)

References 24 publications

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“…32 Lu et al confirmed that circ_0021977 inhibited the proliferation, migration, and invasion of colorectal cancer cells. 6 To explore the function of circ_0001721, miR-372-3p and MAPK7, we checked its expression level and found that circ_0001721 was conspicuously upregulated, 15 miR-372-3p was low expressed, 21 and MAPK7 was highly expressed in OS tissues and cells, 24 which was in line with a previous report. Our experimental results showed that the down-regulation of circ_0001721 effectively inhibited tumor occurrence.…”

Section: Discussionsupporting

confidence: 85%

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<p>CircRNA Circ_0001721 Promotes the Progression of Osteosarcoma Through miR-372-3p/MAPK7 Axis</p>

Gao¹,

Ma²,

Gao³

et al. 2020

CMAR

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Background: Osteosarcoma (OS) is the most common bone tumor. Many studies have reported that circular RNAs (circRNAs) play an important role in the development of a variety of human cancers. However, the underlying mechanism of circ_0001721 in regulating osteosarcoma progression remains unknown. Materials and Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the levels of circ_0001721, miR-372-3p, and mitogen-activated protein kinase 7 (MAPK7) in osteosarcoma tissues and cells. Besides, glycolysis was investigated by glucose consumption, lactate production and hexokinase II (HK2) protein level. Cell proliferation and apoptosis were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and flow cytometry, separately. Cell migration and invasion were determined by transwell assay. Moreover, the protein levels of HK2 and epithelial-to-mesenchymal transition (EMT) markers were determined by Western blot analysis. The relationship between miR-372-3p and circ_0001721 or MAPK7 was predicated by starbase v3.0 and confirmed by dualluciferase reporter assay or RNA binding protein immunoprecipitation (RIP) assay. Murine xenograft model was established to investigate the role of circ_0001721 in vivo. Results: The levels of circ_0001721 and MAPK7 were upregulated in osteosarcoma tissues and cells, while miR-372-3p was downregulated. Knockdown of circ_0001721 inhibited glycolysis, cell proliferation, cell migration, invasion and epithelial-to-mesenchymal transition (EMT), and promoted apoptosis. Circ_0001721 was validated as a sponge of miR-372-3p and mediated glycolysis, cell proliferation, apoptosis, migration, invasion, and EMT of osteosarcoma cells through miR-372-3p. MAPK7 was a target of miR-372-3p and overexpression of MAPK7 attenuated anti-cancer role of miR-372-3p in OS cells. Further studies revealed that circ_0001721 regulates MAPK7 expression via sponging miR-372-3-p. Finally, knockdown of circ_0001721 inhibited tumor progression in vivo. Conclusion: Circ_0001721 promoted osteosarcoma development through the miR-372-3p/ MAPK7 axis.

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Section: Discussionsupporting

confidence: 85%

“…Related studies showed that MAPK7 was involved in the development of a variety of cancers, including OS. 24 Zhou et al proved that MAPK7 was involved in the proliferation and apoptosis of lung cancer. 25 Dong et al reported that miR-143 regulates the proliferation and migration of osteosarcoma cells through targeting MAPK7.…”

Section: Introductionmentioning

confidence: 99%

<p>CircRNA Circ_0001721 Promotes the Progression of Osteosarcoma Through miR-372-3p/MAPK7 Axis</p>

Gao¹,

Ma²,

Gao³

et al. 2020

CMAR

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“…Previous reports have shown that miR-143-3p was abnormally expressed in various cancers [22,23]. In melanoma, Panza et al revealed that miR-143-3p was diminished and miR-143-3p elevation impeded melanoma cell growth and motility and induced apoptosis by binding to COX-2 [14].…”

Section: Discussionmentioning

confidence: 99%

Circ-FOXM1 contributes to cell proliferation, invasion, and glycolysis and represses apoptosis in melanoma by regulating miR-143-3p/FLOT2 axis

et al. 2020

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Background: Numerous literatures have demonstrated that circular RNAs (circRNAs) are involved in multiple types of tumors. However, the effects of circRNAs in melanoma are not very clear. In this study, we aimed to investigate the roles and mechanisms of circ-FOXM1 in melanoma. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to determine the expression of circ-FOXM1, microRNA-143-3p (miR-143-3p), and Flotillin 2 (FLOT2) mRNA. 3-(4,5-Dimethyl-2-thiazolyl)-2,5diphenyl-2-H-tetrazolium bromide (MTT) assay, flow cytometry analysis, and transwell assay were employed to test cell proliferation, apoptosis, and invasion, respectively. The glucose consumption and lactate production were examined by specific kits. Western blot assay was utilized for the detection of hexokinase2 (HK2), pyruvate kinase isozyme type M2 (PKM2), and FLOT2. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were employed to verify the targeting association between miR-143-3p and circ-FOXM1 or FLOT2. A murine xenograft model was established to explore the effect of circ-FOXM1 in vivo. Results: Circ-FOXM1 was elevated and miR-143-3p was reduced in melanoma tissues and cells. Circ-FOXM1 deficiency impeded cell proliferation, invasion, and glycolysis and facilitated cell apoptosis in melanoma in vitro and tumorigenesis in vivo. Circ-FOXM1 acted as a sponge of miR-143-3p and the impacts of circ-FOXM1 silencing on cell proliferation, apoptosis, invasion, and glycolysis were overturned by miR-143-3p deletion. Moreover, FLOT2 was a target gene of miR-143-3p and FLOT2 overexpression rescued the inhibitory effect of miR-143-3p on melanoma progression. Conclusion: Circ-FOXM1 facilitated the development of melanoma by upregulating FLOT2 through miR-143-3p.

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“…miR-143-3p, a tumor-suppressor miRNA, was reported to be involved in multiple cancers, including renal cell carcinoma [ 20 ], triple-negative breast cancer [ 21 ], and osteosarcoma [ 22 ]. In OSCC, miR-143 inhibition promotes carcinogenesis and development [ 23 ], and the present results show that miR-143-3p expression is reduced in OSCC tissues and cells.…”

Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNA (lncRNA) Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Cell Proliferation and Migration by Regulating miR-143-3p and MAGE Family Member A9 (MAGEA9) in Oral Squamous Cell Carcinoma

Shao

Huo

et al. 2020

Med Sci Monit

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Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

Cited by 23 publications

References 24 publications

<p>CircRNA Circ_0001721 Promotes the Progression of Osteosarcoma Through miR-372-3p/MAPK7 Axis</p>

<p>CircRNA Circ_0001721 Promotes the Progression of Osteosarcoma Through miR-372-3p/MAPK7 Axis</p>

Circ-FOXM1 contributes to cell proliferation, invasion, and glycolysis and represses apoptosis in melanoma by regulating miR-143-3p/FLOT2 axis

Long Non-Coding RNA (lncRNA) Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Cell Proliferation and Migration by Regulating miR-143-3p and MAGE Family Member A9 (MAGEA9) in Oral Squamous Cell Carcinoma

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