Mechanism of neomycin stimulation of d-glucose uptake in rabbit intestinal brush border membrane

Lemaire, Jácques; Maestracci, D.; Laprade, Raynald; Sauvé, Rémy

doi:10.1016/0005-2736(82)90157-2

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…A recent study by Lemaire et al (1982) using membrane vesicles prepared from rabbit intestine concluded that the polycationic nature of neomycin was responsible for the increased hexose uptake which they observed. It was suggested by Lemaire et al (1982) that screening of membrane negative charges by the positively charged neomycin causes an accumulation of anions at the membrane surface resulting in an increase in the transmembrane potential difference and thus hexose uptake. However, Bieberdorf et al (1975) and Elsenhans et al (1983) proposed that cationic compounds rather than stimulating absorption actually inhibit the intestinal absorption of sugar by displacing Na+ from the transport carrier or its vicinity.…”

Section: Discussionmentioning

confidence: 96%

“…The compound is likely to have an extracellular action since it is known to be only weakly absorbed by the intestinal epithelium (Feingold, 1963). A recent study by Lemaire et al (1982) using membrane vesicles prepared from rabbit intestine concluded that the polycationic nature of neomycin was responsible for the increased hexose uptake which they observed. It was suggested by Lemaire et al (1982) that screening of membrane negative charges by the positively charged neomycin causes an accumulation of anions at the membrane surface resulting in an increase in the transmembrane potential difference and thus hexose uptake.…”

Section: Discussionmentioning

confidence: 99%

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Effects of neomycin on galactose absorption across rat jejunum

Debnam

Thompson

1984

British J Pharmacology

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The effects of neomycin sulphate on galactose absorption have been studied using in vivo and in vitro preparations of rat small intestine. Neomycin (10−3m) produced an increase in the maximum transport capacity (Jmax) for the active component of absorption in vivo. The apparent Kt for absorption was unaffected. The antibiotic caused a dose‐dependent increase in the potential difference across the mucosal membrane (Vm) measured in vitro, a maximal effect being seen at a concentration of 10 −4m. Furthermore, the magnitude of the depolarization induced by the addition of galactose (4 mm) to the mucosal fluid was enhanced by neomycin (10−4m). Phlorhizin (10 −4m) abolished the galactose‐induced depolarization in both the absence and presence of the antibiotic. It is concluded that neomycin increases the electrical driving force for Na+ during Na+‐coupled galactose entry into the enterocyte.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Effects of neomycin on galactose absorption across rat jejunum

Debnam

Thompson

1984

British J Pharmacology

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“…Механізм, що лежить в основі ідіопатичної (не інфекційної природи) ААД до кінця не з'ясований, зокрема не встанов-лено роль транспортерів води та електролітів епітелію товстої кишки. Крім того, дані про вплив антибіотиків на транспорт води елек-тролітів в кишечнику були отримані при безпосередньому їх додаванні в омиваючий розчин у дослідах in vitro та in situ [2][3][4][5] і не враховували системний ефект.…”

Section: вступunclassified

The Role of Histamine in the Mechanism of Antibiotic-Induced Changes in Colonic Ion and Water Transport

Dovbynchuk¹,

Chervinska²,

Zakordonets³

et al. 2015

Fiziol Zh

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“…Early in-vitro studies suggested that neomycin could stimulate the uptake of D-glucose by rabbit small intestinal brush-border membranes because of the positively charged nature of the antibiotic molecule (Lemaire et al 1982). In contrast, candicidin, a polyene antibiotic, reduced glucose transport by rat small intestinal epithelium because of increased Na+ movement across the apical membrane producing a decrease in its electrochemical gradient (Roschina et al 1980).…”

mentioning

confidence: 99%

Effects of Antibiotics on Epithelial Ion Transport in the Rabbit Distal Colon In-vitro

Goldhill¹,

Rose

Percy

1996

Journal of Pharmacy and Pharmacology

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One side-effect of the therapeutic use of antimicrobial agents is respiratory paralysis as a result of inhibition of skeletal neuromuscular transmission; cholinergic neuro-effector motor transmission in the gastrointestinal tract is inhibited by the same classes of antimicrobial agent. Study of the effects of several classes of antibiotic compound on intestinal motility has suggested that antibiotic-induced alterations of intestinal motility may be related to the onset of diarrhoea or the development of antibiotic-associated colitis. These compounds may, however, also initiate or exacerbate diarrhoea by altering control of epithelial function, a possibility that has not previously been rigorously investigated. This series of experiments investigated the effect of six antibiotics on rabbit distal colonic epithelial ion transport. Of all the antibiotics studied, only ampicillin was without effect. Clindamycin, erythromycin, gentamicin and lincomycin, each reduced the response of the epithelium to electrical field stimulation. In addition, the lincosamides clindamycin and lincomycin reduced basal short circuit current and the epithelial response to acetylcholine. Vancomycin had no effect on the response to electrical field stimulation or acetylcholine but enhanced the secretory action of prostaglandin E2. These data suggest that, in addition to their ability to alter intestinal motility, a number of potential antibiotic interactions with the epithelium and its innervation may contribute to the pathogenesis of antibiotic-associated diarrhoea and colitis.

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Mechanism of neomycin stimulation of d-glucose uptake in rabbit intestinal brush border membrane

Cited by 5 publications

References 42 publications

Effects of neomycin on galactose absorption across rat jejunum

Effects of neomycin on galactose absorption across rat jejunum

The Role of Histamine in the Mechanism of Antibiotic-Induced Changes in Colonic Ion and Water Transport

Effects of Antibiotics on Epithelial Ion Transport in the Rabbit Distal Colon In-vitro

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