Mechanism of NMDA receptor contribution to axon‐to‐glia signaling in the crayfish medial giant nerve fiber

Gafurov, Boris; Urazaev, A. Kh.; Grossfeld, Robert M.; Lieberman, Edward M.

doi:10.1002/glia.10042

Cited by 6 publications

(2 citation statements)

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“…One of the initial characterizations of NMDA receptors from crustaceans was performed in the crayfish optic lobe [75]. Since then, NMDA receptors have been studied in crustaceans for their role in memory [76], axon-to-glial signaling [77], and central pattern generation [78]. We were able to identify 4 separate NMDA receptors from both C. borealis and H. americanus , which fell into two primary categories: 1-like and 2-like (Table 4; Fig.…”

Section: Resultsmentioning

confidence: 99%

Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation

et al. 2016

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BackgroundCrustaceans have been studied extensively as model systems for nervous system function from single neuron properties to behavior. However, lack of molecular sequence information and tools have slowed the adoption of these physiological systems as molecular model systems. In this study, we sequenced and performed de novo assembly for the nervous system transcriptomes of two decapod crustaceans: the Jonah crab (Cancer borealis) and the American lobster (Homarus americanus).ResultsForty-two thousand, seven hundred sixty-six and sixty thousand, two hundred seventy-three contigs were assembled from C. borealis and H. americanus respectively, representing 9,489 and 11,061 unique coding sequences. From these transcripts, genes associated with neural function were identified and manually curated to produce a characterization of multiple gene families important for nervous system function. This included genes for 34 distinct ion channel types, 17 biogenic amine and 5 GABA receptors, 28 major transmitter receptor subtypes including glutamate and acetylcholine receptors, and 6 gap junction proteins – the Innexins.ConclusionWith this resource, crustacean model systems are better poised for incorporation of modern genomic and molecular biology technologies to further enhance the interrogation of fundamentals of nervous system function.

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Section: Resultsmentioning

confidence: 99%

Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation

et al. 2016

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“…One of the initial characterizations of NMDA receptors from crustaceans was performed in the crayfish optic lobe (Pfeiffer-Linn and Glantz, 1991). Since then, NMDA receptors have been studied in crustaceans for their role in memory (Troncoso & Maldonado, 2002), axon-to-glial signaling (Gafurov, Urazaev, Grossfeld, & Lieberman, 2002), and central pattern generation (Cleland & Selverston, 1995).…”

Section: Ionotropic Glutamate Receptorsmentioning

confidence: 99%

The molecular underpinnings of neuronal cell identity in the stomatogastric ganglion of cancer borealis

Northcutt¹

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Throughout the life of an organism, the nervous system must be able to balance changing in response to environmental stimuli with the need to produce reliable, repeatable activity patterns to create stereotyped behaviors. Understanding the mechanisms responsible for this regulation requires a wealth of knowledge about the neural system, ranging from network connectivity and cell type identification to intrinsic neuronal excitability and transcriptomic expression. To make strides in this area, we have employed the well-described stomatogastric nervous system of the Jonah crab Cancer borealis to examine the molecular underpinnings and regulation of neuron cell identity. Several crustacean circuits, including the stomatogastric nervous system and the cardiac ganglion, continue to provide important new insights into circuit dynamics and modulation (Diehl, White, Stein, and Nusbaum, 2013; Marder, 2012; Marder and Bucher, 2007; Williams et al., 2013), but this work has been partially hampered by the lack of extensive molecular sequence knowledge in crustaceans. Here we generated de novo transcriptome assembly from central nervous system tissue for C. borealis producing 42,766 contigs, focusing on an initial identification, curation, and comparison of genes that will have the most profound impact on our understanding of circuit function in these species. This included genes for 34 distinct ion channel types, 17 biogenic amine and 5 GABA receptors, 28 major transmitter receptor subtypes including glutamate and acetylcholine receptors, and 6 gap junction proteins -- the Innexins. ... With this reference transcriptome and annotated sequences in hand, we sought to determine the strengths and limitations of using the neuronal molecular profile to classify them into cell types. ... Since the resulting activity of a neuron is the product of the expression of ion channel genes, we sought to further probe the expression profile of neurons across a range of cell types to understand how these patterns of mRNA abundance relate to the properties of individual cell types. ... Finally, we sought to better understand the molecular underpinnings of how these correlated patterns of mRNA expression are generated and maintained.

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Synthesis of N-Acetylaspartylglutamate (NAAG) and N-Acetylaspartate (NAA) in Axons and Glia of the Crayfish Medial Giant Nerve Fiber

Lieberman¹,

Achreja²,

Urazaev³

Advances in Experimental Medicine and Biology

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Mechanism of NMDA receptor contribution to axon‐to‐glia signaling in the crayfish medial giant nerve fiber

Cited by 6 publications

References 30 publications

Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation

Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation

The molecular underpinnings of neuronal cell identity in the stomatogastric ganglion of cancer borealis

Synthesis of N-Acetylaspartylglutamate (NAAG) and N-Acetylaspartate (NAA) in Axons and Glia of the Crayfish Medial Giant Nerve Fiber

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