Mechanism of PEDF promoting the proliferation of lens epithelial cells in human eyes

Yang, Wenlei; Zhang, Lin

doi:10.1016/j.apjtm.2015.10.009

Cited by 3 publications

(11 citation statements)

References 25 publications

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“…In the human eye, it is thought that PEDF can act as a cytotropic factor to ensure the survival of lens epithelial cells due to its anti‐apoptosis characteristics. The reduction of PEDF content in lens epithelial cells may modulate the pathophysiologic process and cataract pathogenesis of lens cells …”

Section: Discussionmentioning

confidence: 99%

“…Synthesis and secretion of PEDF has been shown to significantly decrease in aged cells in studies of epithelial cell cultures . PEDF is thought to be involved in the pathogenesis and development of some age‐related diseases . Liu et al demonstrated that PEDF levels decrease with age and the decrease shows a significant correlation with age.…”

Section: Discussionmentioning

confidence: 99%

“…PEDF has been reported to be a neurotrophic factor and has also been shown to be a potent inhibitor of angiogenesis. 14 Yang et al 17 prepared a culture from lens anterior capsule samples taken from patients with senile cataract and demonstrated that the apoptotic ratio in a PEDFadded cell culture group was significantly decreased compared to the control group. In the human eye, it is thought that PEDF can act as a cytotropic factor to ensure the survival of lens epithelial cells due to its anti-apoptosis characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…PEDF acts as a survival factor, protecting neuronal cells from natural and induced apoptosis . As a result of these studies, it has been shown that PEDF inhibits oxidation and apoptosis in lens fibril cells, maintains biological activity of the lens, has a protective role in the proliferation and differentiation of lens fibril cells, and has a paracrine or autocrine effect in the growth and development of the lens …”

mentioning

confidence: 99%

“…14 As a result of these studies, it has been shown that PEDF inhibits oxidation and apoptosis in lens fibril cells, maintains biological activity of the lens, has a protective role in the proliferation and differentiation of lens fibril cells, and has a paracrine or autocrine effect in the growth and development of the lens. [14][15][16][17] The present study aims to determine the level of PEDFwhich is thought to be an anti-apoptotic and anti-oxidative protective factorin the lens anterior capsule material of patients with senile cataract and pseudoexfoliation.…”

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confidence: 99%

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Pigment epithelial‐derived factor in the lens anterior capsule of patients with senile cataract with pseudoexfoliation

Utlu

Salman

Öztürk

2020

Clinical and Experimental Optometry

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Background This study sought to evaluate pigment epithelial‐derived factor (PEDF) levels in lens anterior capsule material taken during cataract surgery from patients with senile cataract with pseudoexfoliation. Methods The study included 90 eyes of 86 patients who were diagnosed with, and underwent surgery for, cataracts. Sixty of the eyes included in the study had senile cataract. Thirty eyes of 30 young patients with other forms of cataract were included as a control group. Pseudoexfoliation was present in 21 patients with senile cataract. PEDF levels in the lens anterior capsule material – extracted with capsulorhexis in the classical phacoemulsification procedure – were measured by the enzyme‐linked immunosorbent assay method and compared between the groups. Results The PEDF level in the lens anterior capsule in the senile cataract patient group was 149.36 ± 17.46 pg/ml. A statistically significant lower level of PEDF was found in the lens anterior capsule of patients with senile cataract compared with the other groups. In the patient group with pseudoexfoliation, the PEDF level in the lens anterior capsule was found to be statistically significantly lower than the patient group without pseudoexfoliation. Conclusion PEDF levels decrease with senile cataract and pseudoexfoliation. These findings may clarify the pathogenesis of these conditions and point toward alternative treatment modalities.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Pigment epithelial‐derived factor in the lens anterior capsule of patients with senile cataract with pseudoexfoliation

Utlu

Salman

Öztürk

2020

Clinical and Experimental Optometry

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Protective effect of syringic acid via restoring cells biomechanics and organelle structure in human lens epithelial cells

Yang

Mei

et al. 2021

J Bioenerg Biomembr

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We have previously reported that syringic acid (SA) extracted from D. aurantiacum var. denneanum (kerr) may be used to prevent diabetic cataract (DC). However, the underlying mechanisms through which SA prevents DC in human lens epithelial cells (HLECs) remained unclear. In the present study, we employed single-molecule optics technologies, including transmission electron microscopy (TEM), atomic force microscopy (AFM), laser scanning confocal microscopy (LSCM) and Raman spectroscopy, to monitor the effect of SA on HLECs biomechanics and organelle structure in real-time. TEM suggested that SA improved the ultrastructure of HLECs with regard to nuclear chromatin condensation and reducing mitochondrial swelling and degeneration, which may aid in the maintenance of HLECs integrity in the presence of glucose. AFM revealed a reduced surface roughness and stiffness following SA treatment, suggesting an improved viscoelasticity of HELCs. Raman spectrometry and LSCM further revealed that these changes were related to a modification of cell liquidity and cytoskeletal structure by SA. Taken together, these results provide insights into the effects of SA on the biomechanics of HLECs and further strengthen the evidence for its potential use as a novel therapeutic strategy for DC prevention.

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The human lens is capable of trilineage differentiation towards osteo-, chondro-, and adipogenesis—a model for studying cataract pathogenesis

Boix‐Lemonche

Nagymihály

Lumi

et al. 2023

Front. Bioeng. Biotechnol.

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The potential for trilineage differentiation of cells in tissues represents a model for studying disease pathogenesis and regeneration pathways. Human lens trilineage differentiation has not yet been demonstrated, and so has calcification and osteogenic differentiation of human lens epithelial cells in the whole human lens. Such changes can pose a risk for complications during cataract surgery. Human lens capsules (n = 9) from cataract patients undergoing uneventful surgery were trilineage-differentiated toward osteogenesis, chondrogenesis, and adipogenesis. Furthermore, whole human healthy lenses (n = 3) collected from cadaveric eyes were differentiated into bone and characterized by immunohistochemistry. The cells in the human lens capsules were capable of undergoing trilineage differentiation, while the whole human healthy lenses could undergo osteogenesis differentiation, expressing osteocalcin, collagen I, and pigment epithelium-derived factor. We, hereby, show an ex vivo model for cataract formation through different stages of opacification, as well as provide in vivo evidence from patients undergoing calcified lens extraction with bone-like consistency.

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Mechanism of PEDF promoting the proliferation of lens epithelial cells in human eyes

Abstract: In human eyes, PEDF may function as cytotropic factor to promote survival of LECs through anti-apoptosis and reducing-expression of VEGF. Decrease of PEDF content in LECs probably modulates the pathophysiological process of lens cells and further cataractogenesis.

Cited by 3 publications

References 25 publications

Pigment epithelial‐derived factor in the lens anterior capsule of patients with senile cataract with pseudoexfoliation

Pigment epithelial‐derived factor in the lens anterior capsule of patients with senile cataract with pseudoexfoliation

Protective effect of syringic acid via restoring cells biomechanics and organelle structure in human lens epithelial cells

The human lens is capable of trilineage differentiation towards osteo-, chondro-, and adipogenesis—a model for studying cataract pathogenesis

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