Mechanism of protective immunity by vaccination with recombinant Echinococcus granulosus glutathione S-transferase (Chinese strain) in mice

Abstract

The aim of this study was to investigate the immunoprotective effects of recombinant glutathione S-transferase (rEgGST) against the development of protoscolices (PSCs), and to determine the mechanisms underlying this protection. ICR mice were subcutaneously immunized three times with rEgGST at weeks 0, 2 and 4, followed by the intraperitoneal administration of PSCs at week 10. Six mice in each group were sacrificed at 0, 2, 4, 6, 10, 18 and 30 weeks following the initial vaccination in order to observe the macroscopic and microscopic effects of parasite development. Various analyses were subsequently conducted, including determination of the levels of immunoglobulins (Igs) and cytokines. Significant differences were observed a number of indices of immune response following immunization with rEgGST. These included reduced cyst formation and elevated levels of IgG1, IgG2a, IgG3, IL-2, IL-4, IL-10 and IFN-γ, which indicated an increased percentage of immune helper cells. The results of the present study suggest that immunization with rEgGST in mice is able to successfully reduce the PSC-induced formation of cysts and to stimulate an immune response, suggesting that rEgGST possesses potential value as a candidate vaccine for PSC infection.

Efficacy of Purified Glutathione -S- Transferee in Providing Protection against Haemonchus Contortus Infection in Sheep

“…The previous studies on GSTs from helminthes were documented its induction of immune protection, Fasciola hepatica, Seteria cervi and Echinococcus granulosus [37][38][39]. Therefore, here in, a trial for lamb vaccination by purified GST of H. contortus was conducted.…”

Screening of antigenic vesicular fluid proteins of Echinococcus multilocularis as potential viability biomarkers to monitor drug response in alveolar echinococcosis patients

“…The function of this protein, corresponding to EmCBP1, was recently characterized . Glutathione S‐transferase has been widely studied in cystic echinoccosis, and this protein has been assessed for its vaccine potential against CE in mice . Another elongation factor, EF‐1beta/delta, was described for its immunological properties in E. granulosus infection, and antibodies against this elongation factor were associated with degenerating metacestodes in humans .…”

Treatment of protoscoleces with gamma radiation: Potential immunoprotective effect against experimental murine echinococcosis

“…Therefore, finding new potential vaccine candidates or effective drugs became urgently to prevent infection, transmission by stopping the parasite's development at a specific stage of its cycle, and recurrence risks, which can occur when cysts rupture or operative leakage occurs, resulting in the dissemination of viable protoscolices to adjacent tissues and the development of secondary echinococcosis. 9 Indeed, some vaccine candidates evaluated in mice or ruminantsb s [10][11][12][13][14][15][16][17][18][19] have proven high protective immunity such as EG95 vaccine in experimental immunization trials conducted in seven countries. 20,21 Protoscolex, one component of the larval stages is an important infectious agent that contributes to the transmission of this disease.…”

“…[25][26][27][28][29][30][31][32][33][34][35] They have been also used to investigate the effect of radiation for therapeutic use or preventing echinococcosis recurrence after surgical removal of hydatid cysts [36][37][38][39][40][41] and to assess the potential of many molecules as novel vaccine for preventing infection. 9,16,17 Our study was conducted to investigate the immunoprotective effect against experimental murine echinococcosis by using gamma rays irradiated protoscoleces.…”