Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid.

Enoch, Harry G.; Catalá, Ángel; Strittmatter, Philipp

doi:10.1016/s0021-9258(17)33223-4

Cited by 523 publications

(84 citation statements)

References 56 publications

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“…A critical committed step in the biosynthesis of monounsaturated fatty acids is the introduction of the first cisdouble bond in the ⌬ 9 position (between carbons 9 and 10). This oxidative reaction is catalyzed by the ironcontaining, microsomal enzyme stearoyl-CoA desaturase (SCD) and involves cytochrome b 5 , NADH (P)-cytochrome b 5 reductase, and molecular oxygen ( Fig. 1 ).…”

Section: Stearoyl-c O a Desaturase: What Does It Do?mentioning

confidence: 99%

Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol

Ntambi

1999

Journal of Lipid Research

639

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The lipid composition of cellular membranes is regulated to maintain membrane fluidity. A key enzyme involved in this process is the membrane-bound stearoyl-CoA desaturase (SCD) which is the rate-limiting enzyme in the cellular synthesis of monounsaturated fatty acids from saturated fatty acids. A proper ratio of saturated to monounsaturated fatty acids contributes to membrane fluidity. Alterations in this ratio have been implicated in various disease states including cardiovascular disease, obesity, non-insulindependent diabetes mellitus, hypertension, neurological diseases, immune disorders, and cancer. The regulation of stearoyl-CoA desaturase is therefore of considerable physiological importance and its activity is sensitive to dietary changes, hormonal imbalance, developmental processes, temperature changes, metals, alcohol, peroxisomal proliferators, and phenolic compounds. Two mouse and rat SCD genes (SCD1 and SCD2) and a single human SCD gene have been cloned and characterized. In the past several years we have studied the dietary influences on the genetic expression of the mouse stearoyl-CoA desaturase. The expression of the mouse SCD genes is regulated by polyunsaturated fatty acids and cholesterol at the levels of transcription and mRNA stability. Promoter elements that are responsible for the polyunsaturated fatty acid repression colocalize with the promoter elements for SREBP-mediated regulation of the SCD genes.It is the goal of this review to provide an overview of the genetic regulation of the stearoyl-CoA desaturase in response to dietary polyunsaturated fatty acids and cholesterol. -Ntambi, J. M. Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol.

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Section: Stearoyl-c O a Desaturase: What Does It Do?mentioning

confidence: 99%

Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol

Ntambi

1999

Journal of Lipid Research

639

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“…At later stages, D21 and D56 livers shared two common rare subpopulations, Hep6 and Hep7, but had distinct major subpopulations, Hep5 and Hep8, respectively (Figures 2D-2E). Of note, the Hep3 subpopulation, only identified at D1, was featured by unique expression of Scd2 (Figures 2F, S1A), which encodes one of the four mouse isoforms of stearoyl-CoA desaturase (SCD), a key enzyme for biosynthesis of monounsaturated fatty acids (Enoch et al, 1976;Miyazaki et al, 2005;Miyazaki et al, 2003). Among the four isoforms, SCD1 is the only one identified in adult liver, and is known to play a critical role in lipid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Temporal Analyses of Postnatal Liver Development and Maturation by Single Cell Transcriptomics

Liang

Kaneko

et al. 2021

Preprint

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Liver is the major metabolic organ, although its postnatal development and maturation are inadequately understood. We analyzed 52,834 single cell transcriptomes and identified 31 cell types or states in mouse livers at postnatal day 1, 3, 7, 21 and 56. We observed unexpectedly high levels of hepatocyte heterogeneity in the developing liver and progressive construction of the zonated metabolic functions from pericentral to periportal hepatocytes, which was orchestrated with development of sinusoid endothelial, stellate and Kupffer cells. Trajectory and gene regulatory analyses captured 36 transcription factors, including a circadian regulator Bhlhe40, in programming liver development. Remarkably, we identified a special group of macrophages enriched at day 7 with a hybrid phenotype of macrophages and endothelial cells, which may regulate sinusoidal construction and Treg cell function. This study provides a comprehensive atlas that covers all hepatic cell types instrumental for further dissection of liver development, metabolic functions and diseases.

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“…Stearoyl-coenzyme A desaturase (SCD), also named delta 9 desaturase, is a key lipogenic enzyme found in the endoplasmic reticulum and a rate-limiting enzyme in the biosynthesis of monounsaturated fatty acids from saturated fatty acids [57,58]. Saturated free fatty acids have a dose-dependent negative impact on oocyte developmental competence, while monounsaturated free fatty acids appear less harmful [59].…”

Section: Discussionmentioning

confidence: 99%

Tribbles Pseudokinase 2 (TRIB2) Regulates Expression of Binding Partners in Bovine Granulosa Cells

Warma

Lussier

Ndiaye

2021

IJMS

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Members of the Tribbles (TRIB) family of pseudokinases are critical components of intracellular signal transduction pathways in physiological and pathological processes. TRIBs, including TRIB2, have been previously shown as signaling mediators and scaffolding proteins regulating numerous cellular events such as proliferation, differentiation and cell death through protein stability and activity. However, the signaling network associated with TRIB2 and its binding partners in granulosa cells during ovarian follicular development is not fully defined. We previously reported that TRIB2 is differentially expressed in growing dominant follicles while downregulated in ovulatory follicles following the luteinizing hormone (LH) surge or human chorionic gonadotropin (hCG) injection. In the present study, we used the yeast two-hybrid screening system and in vitro coimmunoprecipitation assays to identify and confirm TRIB2 interactions in granulosa cells (GCs) of dominant ovarian follicles (DFs), which yielded individual candidate binding partners including calmodulin 1 (CALM1), inhibin subunit beta A (INHBA), inositol polyphosphate phosphatase-like 1 (INPPL1), 5’-nucleotidase ecto (NT5E), stearoyl-CoA desaturase (SCD), succinate dehydrogenase complex iron sulfur subunit B (SDHB) and Ras-associated protein 14 (RAB14). Further analyses showed that all TRIB2 binding partners are expressed in GCs of dominant follicles but are differentially regulated throughout the different stages of follicular development. CRISPR/Cas9-driven inhibition along with pQE-driven overexpression of TRIB2 showed that TRIB2 differently regulates expression of binding partners, which reveals the importance of TRIB2 in the control of gene expression linked to various biological processes such as proliferation, differentiation, cell migration, apoptosis, calcium signaling and metabolism. These data provide a larger view of potential TRIB2-regulated signal transduction pathways in GCs and provide strong evidence that TRIB2 may act as a regulator of target genes during ovarian follicular development.

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Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid.

Cited by 523 publications

References 56 publications

Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol

Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol

Temporal Analyses of Postnatal Liver Development and Maturation by Single Cell Transcriptomics

Tribbles Pseudokinase 2 (TRIB2) Regulates Expression of Binding Partners in Bovine Granulosa Cells

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