Mechanism of Reduction of Dihydrofolate to Tetrahydrofolate

Zakrzewski, Sigmund F.; Sansone, Annette M.

doi:10.1016/s0021-9258(18)99351-8

Cited by 7 publications

(1 citation statement)

References 14 publications

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“…This correlation of difference spectrum band position with stereospecificity in enzymatic activity was first demonstrated for mitochondrial malate dehydrogenase (Fisher et al, 1969) and was also seen with E. coli dihydrofolate reductase (Poe and Hoogsteen, 1974). The dihydrofolate reductases from mouse LI210 cells (Pastore and Friedkin, 1962), Streptococcus faecalis (Blakley et al, 1963), Sarcoma 180 strain AT/300 (Zakrzewski and Sansone, 1967;Zakrzewski, 1969), and chicken liver and wild-type E. coli B (Pastore and Williamson, 1968) have been also demonstrated to use the A proton of NADPH. The E-NADPH difference spectrum between 270 and 310 nm exhibits a pair of maxima at approximately 280 and 290 nm.…”

Section: Ultraviolet Difference Spectra (1) Enzyme Plus Folatesmentioning

confidence: 99%

Dihydrofolate reductase from a resistant subline of the L1210 lymphoma. Purification by affinity chromatography and ultraviolet difference spectrophotometric and circular dichroic studies

Gupta¹,

Greenfield²,

Poe³

et al. 1977

Biochemistry

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Dihydrofolate reductase has been isolated in 80% yield and purity >99% from a methotrexate-resistant subline of the LI 210 lymphoma (L1210/MTX) by the use of affinity chromatography. Purity has been established by titrating enzymic activity and protein fluorescence with a stoichiometric inhibitor methotrexate (MTX) and confirmed by polyacrylamide gel electrophoresis. The apparent molecular weight calculated from filtration through a Sephadex G-100 column is 21 000 ± 3%.

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Section: Ultraviolet Difference Spectra (1) Enzyme Plus Folatesmentioning

confidence: 99%

Dihydrofolate reductase from a resistant subline of the L1210 lymphoma. Purification by affinity chromatography and ultraviolet difference spectrophotometric and circular dichroic studies

Gupta¹,

Greenfield²,

Poe³

et al. 1977

Biochemistry

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An improved synthesis of homopteroic and homofolic acids

Kim¹,

Grubliauskas²,

Friedman³

1972

Journal of Heterocyclic Chem

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A more practical synthesis of homopteroic and homofolic acids involving condensation of 2,4,5‐triamino‐6(1H)pyrimidinone (3) with 1‐acetoxy‐4‐[N‐acetyl‐(p‐carbethoxyphenyl)amino]‐2‐butanone (7) is described. The biological activities of homofolic (1‐b) and homopteroic (2‐b) acids were compared and found to be identical with the activities of these products prepared by the unambiguous route.

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