Mechanism of seedling protection induced by a hypovirulent isolate of <i>Rhizoctonia solani</i>

Sneh, B.; Ichielevich-Auster, Meira; Plaut, Z.

doi:10.1139/b89-270

Cited by 27 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The alteration in ROSscavenging enzymes could be a vital process in phytopathogen defense (Gara et al 2003). Non-pathogenic microbial inoculation can heighten the resistance of host plants against their pathogens by an increase of phenolic compounds (Gasoni and Gurfinkel 2009;Sneh et al 1989). In support of this, we found that the phenolic content of R. solani-infected plants was less than that of the controls, but again, B. amyloliquefaciens subsp.…”

Section: Discussionsupporting

confidence: 57%

Bacillus amyloliquefaciens subsp. plantarum GR53, a potent biocontrol agent resists Rhizoctonia disease on Chinese cabbage through hormonal and antioxidants regulation

Kang

Radhakrishnan

Lee

2015

World J Microbiol Biotechnol

View full text Add to dashboard Cite

The fungus Rhizoctonia solani is one of the causal agents of numerous diseases that affect crop growth and yield. The aim of this present investigation was to identify a biocontrol agent that acts against R. solani and to determine the agent's protective effect through phytohormones and antioxidant regulation in experimentally infected Chinese cabbage plants. Four rhizospheric soil bacterial isolates GR53, GR169, GR786, and GR320 were tested for their antagonistic activity against R. solani. Among these isolates, GR53 significantly suppressed fungal growth. GR53 was identified as Bacillus amyloliquefaciens subsp. plantarum by phylogenetic analysis of the 16S rDNA sequence. The biocontrol activity of B. amyloliquefaciens subsp. plantarum GR53 was tested in Chinese cabbage plants under controlled conditions. Results showed that R. solani inhibited plant growth (length, width, fresh and dry weight of leaves) by reducing chlorophyll and total phenolic content, as well as by increasing the levels of salicylic acid, jasmonic acid, abscisic acid, and DPPH scavenging activity. By regulating the levels of these compounds, the co-inoculation of B. amyloliquefaciens subsp. plantarum GR53 heightened induced systemic resistance in infected Chinese cabbage, effectively mitigating R. solani-induced damaging effects and improving plant growth. The results obtained from this study suggest that B. amyloliquefaciens subsp. plantarum GR53 is an effective biocontrol agent to prevent the damage caused by R. solani in Chinese cabbage plants.

show abstract

Section: Discussionsupporting

confidence: 57%

Bacillus amyloliquefaciens subsp. plantarum GR53, a potent biocontrol agent resists Rhizoctonia disease on Chinese cabbage through hormonal and antioxidants regulation

Kang

Radhakrishnan

Lee

2015

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Today, it is well established that Rhizoctonia is significantly affected by suppressive organisms, but the nature of this biocontrol is still a matter of debate [4]. The finding that hypovirulent isolates of Rhizoctonia can be successfully used for biocontrol purposes, raised also interest in the possible involvement of mycoviruses [5,6].…”

Section: Introductionmentioning

confidence: 99%

Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV

Bartholomäus¹,

Wibberg

Winkler

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Rhizoctonia solani represents an important plant pathogenic Basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsRNA elements in natural populations of the fungus. To date, eight different mycoviruses have been characterized in Rhizoctonia and some of them have been reported to modulate its virulence. DsRNA extracts of the avirulent R. solani isolate DC17 (AG2-2-IV) displayed a diverse pattern, indicating multiple infections with mycoviruses. Deep sequencing analysis of the dsRNA extract, converted to cDNA, revealed that this isolate harbors at least 17 different mycovirus species. Based on the alignment of the conserved RNA-dependent RNA-polymerase (RdRp) domain, this viral community included putative members of the families Narnaviridae, Endornaviridae, Partitiviridae and Megabirnaviridae as well as of the order Tymovirales. Furthermore, viruses, which could not be assigned to any existing family or order, but showed similarities to so far unassigned species like Sclerotinia sclerotiorum RNA virus L, Rhizoctonia solani dsRNA virus 1, Aspergillus foetidus slow virus 2 or Rhizoctonia fumigata virus 1, were identified. This is the first report of a fungal isolate infected by 17 different viral species and a valuable study case to explore the diversity of mycoviruses infecting R. solani.

show abstract

“…Davis et al (2010) showed that a decrease of V. dahliae wilt on potato correlated with an increase in root colonization by non-pathogenic Fusarium equiseti. Sneh et al (1989) reported that the hypovirulent Rhizoctonia sp. isolate 521 provided significant protection on radish against virulent Rhizoctonia strains by densely colonizing the root surface.…”

Section: Competitionmentioning

confidence: 99%

Microbiome dynamics of disease suppresive soils

Expósito¹

View full text Add to dashboard Cite

The genus Lysobacter includes several species that produce a range of extracellular enzymes and other metabolites with activity against bacteria, fungi, oomycetes and nematodes. Lysobacter species were found to be more abundant in soil suppressive against the fungal root pathogen Rhizoctonia solani, but their actual role in disease suppression is still unclear. Here, the antifungal and plant growth-promoting activities of 18Lysobacter strains, including 11 strains from Rhizoctonia suppressive soils, were studied both in vitro and in vivo. Based on 16S rRNA sequencing, the Lysobacter strains from the Rhizoctonia suppressive soil belonged to the four species L. antibioticus, L. capsici, L. enzymogenes and L. gummosus. Most strains showed strong in vitro activity against R. solani and several other pathogens, including Pythium ultimum, Aspergillus niger, Fusarium oxysporum and Xanthomonas campestris. When the Lysobacter strains were introduced into soil, however, no significant and consistent suppression of R. solani damping-off disease of sugar beet and cauliflower was observed. Subsequent bioassays further revealed that none of the Lysobacter strains was able to promote growth of sugar beet, cauliflower, onion and Arabidopsis thaliana, either directly or via volatile compounds. The lack of in vivo activity is most likely attributed to poor colonization of the rhizosphere by the introduced Lysobacter strains. In conclusion, our results demonstrated that Lysobacter species have strong antagonistic activities against a range of pathogens, making them an important source for putative new enzymes and antimicrobial compounds. However, their potential role in R. solani disease suppressive soil could not be confirmed. In-depth omics'-based analyses will be needed to shed more light on the potential contribution of Lysobacter species to the collective activities of microbial consortia in disease suppressive soils.

show abstract

Mechanism of seedling protection induced by a hypovirulent isolate of Rhizoctonia solani

Cited by 27 publications

References 20 publications

Bacillus amyloliquefaciens subsp. plantarum GR53, a potent biocontrol agent resists Rhizoctonia disease on Chinese cabbage through hormonal and antioxidants regulation

Bacillus amyloliquefaciens subsp. plantarum GR53, a potent biocontrol agent resists Rhizoctonia disease on Chinese cabbage through hormonal and antioxidants regulation

Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV

Microbiome dynamics of disease suppresive soils

Contact Info

Product

Resources

About