Mechanism of Spontaneous Activity in Afferent Neurons of the Zebrafish Lateral-Line Organ

Trapani, Josef G.; Nicolson, Teresa

doi:10.1523/jneurosci.3369-10.2011

Cited by 75 publications

(98 citation statements)

References 74 publications

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“…Reduced auditory sensitivity can occur due to mechanotransduction defects, or deficits further downstream such as synaptic dysfunction ( e.g., Söllner et al, 2004; Trapani and Nicolson, 2011). The vital dye FM1-43 enters hair cells via the mechanotransduction channel, and therefore dye uptake is considered a proxy for channel function (Meyers et al, 2003; Park et al, 2013; Uribe et al, 2013b).…”

Section: Resultsmentioning

confidence: 99%

Hearing sensitivity differs between zebrafish lines used in auditory research

et al. 2016

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Zebrafish are increasingly used in auditory studies, in part due to the development of several transgenic lines that express hair cell-specific fluorescent proteins. However, it is largely unknown how transgene expression influences auditory phenotype. We previously observed reduced auditory sensitivity in adult Brn3c:mGFP transgenic zebrafish, which express membrane-bound green fluorescent protein (GFP) in sensory hair cells. Here, we examine the auditory sensitivity of zebrafish from multiple transgenic and background strains. We recorded auditory evoked potentials in adult animals and observed significantly higher auditory thresholds in three lines that express hair cell-specific GFP. There was no obvious correlation between hair cell density and auditory thresholds, suggesting that reduced sensitivity was not due to a reduction in hair cell density. FM1-43 uptake was reduced in Brn3c:mGFP fish but not in other lines, suggesting that a mechanotransduction defect may be responsible for the auditory phenotype in Brn3c animals, but that alternate mechanisms underlie the increased AEP thresholds in other lines. We found reduced prepulse inhibition (a measure of auditory-evoked behavior) in larval Brn3c animals, suggesting that auditory defects develop early in this line. We also found significant differences in auditory sensitivity between adults of different background strains, akin to strain differences observed in mouse models of auditory function. Our results suggest that researchers should exercise caution when selecting an appropriate zebrafish transgenic or background strain for auditory studies.

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Section: Resultsmentioning

confidence: 99%

Hearing sensitivity differs between zebrafish lines used in auditory research

et al. 2016

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“…In the absence of an experimentally applied stimulus, we observed spontaneous action potentials in SGNs (Fig. 1C), which are thought to be initiated by Ca 2ϩ -dependent synaptic transmission from hair cells both in vitro [rat (Glowatzki and Fuchs, 2002); frog (Keen and Hudspeth, 2006); fish (Trapani and Nicolson, 2011)] and in vivo [cat (Liberman and Kiang, 1978;Sewell, 1984); chinchilla (Siegel and Relkin, 1987); guinea pig (Robertson and Paki, 2002)]. When action potentials originated from baseline potentials of approximately Ϫ80 mV, mean spike heights ranged from 30 to 90 mV and spike FWHM ranged from 0.42 to 1.5 ms between cells (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Spike Encoding of Neurotransmitter Release Timing by Spiral Ganglion Neurons of the Cochlea

2012

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Mammalian cochlear spiral ganglion neurons (SGNs) encode sound with microsecond precision. Spike triggering relies upon input from a single ribbon-type active zone of a presynaptic inner hair cell (IHC). Using patch-clamp recordings of rat SGN postsynaptic boutons innervating the modiolar face of IHCs from the cochlear apex, at room temperature, we studied how spike generation contributes to spike timing relative to synaptic input. SGNs were phasic, firing a single short-latency spike for sustained currents of sufficient onset slope. Almost every EPSP elicited a spike, but latency (300 -1500 s) varied with EPSP size and kinetics. When current-clamp stimuli approximated the mean physiological EPSC (Ϸ300 pA), several times larger than threshold current (rheobase, Ϸ50 pA), spikes were triggered rapidly (latency, Ϸ500 s) and precisely (SD, Ͻ50 s). This demonstrated the significance of strong synaptic input. However, increasing EPSC size beyond the physiological mean resulted in less-potent reduction of latency and jitter. Differences in EPSC charge and SGN baseline potential influenced spike timing less as EPSC onset slope and peak amplitude increased. Moreover, the effect of baseline potential on relative threshold was small due to compensatory shift of absolute threshold potential. Experimental first-spike latencies in response to a broad range of stimuli were predicted by a two-compartment exponential integrate-and-fire model, with latency prediction error of Ͻ100 s. In conclusion, the close anatomical coupling between a strong synapse and spike generator along with the phasic firing property lock SGN spikes to IHC exocytosis timing to generate the auditory temporal code with high fidelity.

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“…Furthermore, no other species offers a greater potential for understanding the functioning of the lateral line system (e.g. Kohashi and Oda, 2008;Nagiel et al, 2009;Nuñez et al, 2009;Mo and Nicolson, 2011;Trapani and Nicolson, 2011) and the startle response (e.g. Bhatt et al, 2007;Issa et al, 2011;Nikolaou and Meyer, 2012;Liu et al, 2012) across levels of organization.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish larvae evade predators by sensing water flow

Stewart

Cardenas

McHenry

2013

Journal of Experimental Biology

172

168

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SUMMARYThe ability of fish to evade predators is central to the ecology and evolution of a diversity of species. However, it is largely unclear how prey fish detect predators in order to initiate their escape. We tested whether larval zebrafish (Danio rerio) sense the flow created by adult predators of the same species. When placed together in a cylindrical arena, we found that larvae were able to escape 70% of predator strikes (mean escape probability P escape =0.7, N=13). However, when we pharmacologically ablated the flow-sensitive lateral line system, larvae were rarely capable of escape (mean P escape =0.05, N=11). In order to explore the rapid events that facilitate a successful escape, we recorded freely swimming predators and prey using a custom-built camera dolly. This device permitted two-dimensional camera motion to manually track prey and record their escape response with high temporal and spatial resolution. These recordings demonstrated that prey were more than 3 times more likely to evade a suctionfeeding predator if they responded before (P escape =0.53, N=43), rather than after (P escape =0.15, N=13), a predatorʼs mouth opened, which is a highly significant difference. Therefore, flow sensing plays an essential role in predator evasion by facilitating a response prior to a predatorʼs strike.

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Mechanism of Spontaneous Activity in Afferent Neurons of the Zebrafish Lateral-Line Organ

Cited by 75 publications

References 74 publications

Hearing sensitivity differs between zebrafish lines used in auditory research

Hearing sensitivity differs between zebrafish lines used in auditory research

Spike Encoding of Neurotransmitter Release Timing by Spiral Ganglion Neurons of the Cochlea

Zebrafish larvae evade predators by sensing water flow

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