Mechanism of superoxide anion generation in the toxic red tide phytoplankton Chattonella marina: possible involvement of NAD(P)H oxidase

Kim, Daekyung; Nakamura, Atsushi; Okamoto, Tarou; Komatsu, Nobukazu; Oda, Tatsuya; Iida, Takaji; Ishimatsu, Atsushi; Muramatsu, Tsuyoshi

doi:10.1016/s0304-4165(00)00161-6

Cited by 94 publications

(89 citation statements)

References 31 publications

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“…However, AACOCF3 (10 μM) and BPB (10 μM) did not have an influence. In addition, DPI, an inhibitor of the NADPH oxidase (Kim et al, 2000) almost completely suppressed the NEM-induced ROS increase, which is consistent with the results of a previous study ), demonstrating that the NADPH oxidase mediates the NEM-induced ROS production. Taken together, these results indicate that iPLA2-AA signal may be essentially involved in the NADPH oxidase-mediated ROS production and in turn, KCC activation.…”

Section: Role Of Ipla2-aa Signal In Ros-mediated Kcc Activationsupporting

confidence: 92%

Arachidonic Acid Activates K+-Cl--cotransport in HepG2 Human Hepatoblastoma Cells

Lee

2009

Korean J Physiol Pharmacol

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Section: Role Of Ipla2-aa Signal In Ros-mediated Kcc Activationsupporting

confidence: 92%

Arachidonic Acid Activates K+-Cl--cotransport in HepG2 Human Hepatoblastoma Cells

Lee

2009

Korean J Physiol Pharmacol

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“…, 1994Tanaka & Muto, 1992;Kim et al, 2000). In these studies however, ROS were only measured using relative scales, to demonstrate that exponential growth phase C. marina produced higher levels of ROS than stationary phase cells (Oda et al, 1995;Kawano et al, 1996), or that ROS produced by C. marina in the absence of SOD or CAT were higher than in the presence of these inhibitors (Oda et al, 1994(Oda et al, , 1995.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to Chattonella marina and hydrogen peroxide: Implications on the cause of fish kills

Woo

Liu

et al. 2006

Journal of Experimental Marine Biology and Ecology

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Chattonella marina, a red tide or harmful algal bloom species, has caused mass fish kills and serious economic loss worldwide, and yet its toxic actions remain highly controversial. Previous studies have shown that this species is able to produce reactive oxygen species (ROS), and therefore postulated that ROS are the causative agents of fish kills. The present study investigates antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to C. marina, compared with responses exposed to equivalent and higher levels of ROS exposure.Even though C. marina can produce a high level of ROS, gills and erythrocytes of sea bream exposed to C. marina for 1 to 6 h showed neither significant induction of antioxidant enzymes nor lipid peroxidation. Antioxidant responses and oxidative damage did not occur as fish mortality began to occur, yet could be induced upon exposure to artificially supplied ROS levels an order of magnitude higher. The result of this study implies that ROS produced by C. marina is not the principal cause of fish kills.

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“…Chemiluminescence methods based on the oxidation of luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) by H 2 O 2 and MCLA [2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3(7H)-one hydrochloride] by O 2 -have been respectively used to determine H 2 O 2 concentrations in aquatic ecosystems (Yuan and Shiller 1999) and ROS production by phytoplankton (Lee et al 1995;Kim et al 2000Kim et al , 2004Zheng et al 2003;Kustka et al 2005;Rose et al 2008b). We therefore used these two chemiluminescent probes to develop a flow injection-chemiluminescence system for both H 2 O 2 and O 2 -analyses.…”

Section: Introductionmentioning

confidence: 99%

Real‐time detection of reactive oxygen species generation by marine phytoplankton using flow injection—chemiluminescence

Milne

Davey

Worsfold

et al. 2009

Limnology & Ocean Methods

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Little is known about the biological production of reactive oxygen species (ROS) ). An initial examination of the effect of changing light intensity showed that a rapid light-induced production of both O 2 -and H 2 O 2 by T. weissflogii cells could be readily detected. Moreover, this production was proportional to the biomass present on the flushed filter. These methods enable the monitoring of real-time fluctuations of biological ROS production in response to changing environmental conditions, and therefore facilitate analysis of the biotic component of ROS production and the subsequent impacts on chemical speciation of nutrients and trace metals in aquatic ecosystems.

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Mechanism of superoxide anion generation in the toxic red tide phytoplankton Chattonella marina: possible involvement of NAD(P)H oxidase

Cited by 94 publications

References 31 publications

Arachidonic Acid Activates K+-Cl--cotransport in HepG2 Human Hepatoblastoma Cells

Arachidonic Acid Activates K+-Cl--cotransport in HepG2 Human Hepatoblastoma Cells

Antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to Chattonella marina and hydrogen peroxide: Implications on the cause of fish kills

Real‐time detection of reactive oxygen species generation by marine phytoplankton using flow injection—chemiluminescence

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