Mechanism of the Aldolase Reaction

Intein-mediated purification of a recombinantly expressed peptide

“…This result is consistent with the 20-fold reduction of activity seen upon removal of amino acids at the C-terminus after carboxypeptidase treatment of wild-type aldolase A. 20 After purifying Ald-337, ligation of the C-terminal peptide was tested utilizing the naturally occurring cysteine at position 338, which in this system, is located on the N-terminus of the peptide. Ligation was accomplished by dissolving the lyophilized peptide, to a final concentration of 500 mM, in a 100 mM solution of Ald-337 in 20 mM HEPES, 500 mM NaCl, 1 mM EDTA, pH 8.0.…”

New Stereoselective Biocatalysts for Carboligation and Retro-Aldol Cleavage Reactions Derived from d-Fructose 6-Phosphate Aldolase

“…The kinetic mechanism of FSA has not been extensively studied but is expected to follow a strictly ordered model analogous to that of fructose 1,6-bis-phosphate aldolase, although the identities of the catalytic residues differ between these isoenzymes . In this model, the ketone donor reacts first to form the Schiff base, via a carbinolamine intermediate.…”

A recyclable bifunctional acid–base organocatalyst with ionic liquid character. The role of site separation and spatial configuration on different condensation reactions

“…By means of this bifunctional catalyst the enzyme is able to catalyze the condensation reaction without requiring very strong basic sites. [34][35][36] Scheme 2 Schematic representation of (a) the aldol reaction between dihydroxyacetonephosphate (DHAP) and D-glyceraldehyde-3-phosphate (GAP) catalyzed by the enzyme D-fructose-1,6-biphosphate aldolase (FBPA) and (b) aldol reaction between phenyl-methyl-ketone and benzaldehyde catalyzed by gem-diamine dipiperidinomethane tetrafluoroboric acid 1.…”