Mechanism of the toxic action of cationic G5 and G7 PAMAM dendrimers in the cyanobacterium<i>Anabaena</i>sp. PCC7120

Tamayo-Belda, Miguel; González-Pleiter, Miguel; Pulido-Reyes, Gerardo; Martín-Betancor, Keila; Leganés, Francisco; Rosal, Roberto; Fernández-Piñas, Francisca

doi:10.1039/c8en01409d

Cited by 15 publications

(18 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Five physiological parameters were analysed by FCM (Table 1): membrane integrity (PI), cytoplasmic membrane potential (DiBAC4(3)), nonspecific esterase activity (FDA), intracellular pH (BCECF-AM) and mitochondrial membrane potential (JC-1). The fluorochrome concentrations and incubation times used were as previously reported by Prado et al (2012) (Prado et al, 2012), González-Pleiter et al, 2017(González-Pleiter et al, 2017 and Tamayo-Belda et al, 2019(Tamayo-Belda et al, 2019 and can be found in Table 1. After 72 h of exposure to the EC50 of each SPION, cells were incubated with each fluorochrome at room temperature in the dark prior to flow cytometry analyses.…”

Section: Flow Cytometry Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii

et al. 2020

Self Cite

View full text Add to dashboard Cite

Section: Flow Cytometry Analysesmentioning

confidence: 99%

“…Previous studies have observed lipid peroxidation in algae and cyanobacteria after the exposure to NPs for 72 h: TiO 2 NPs in Chlamydomonas reinhardtii(Chen et al, 2012a;Wang et al, 2008); Fe3O4 on Chlorella vulgaris(Chen et al, 2012b) and cationic G5 and G7 PAMAM dendrimers in the cyanobacterium Anabaena sp. PCC7120(Tamayo- Belda et al, 2019).…”

mentioning

confidence: 99%

Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii

et al. 2020

Self Cite

View full text Add to dashboard Cite

“…Moreover, it is known that the quaternary ammonium moieties efficiently interact with the negatively charged groups of bacterial walls or cytoplasmic membranes, mainly through electrostatic as well as with secondary hydrophobic interactions, leading to dysfunction in cellular processes and probably to cell death [ 62 ]. Highly functionalized polymers with quaternary ammonium groups have been found to be more effective antibacterial agents than their low molecular weight analogues, as their higher charge densities lead to stronger interactions with the negatively charged bacteria walls [ 63 ]. For example, poly(propylene imine) dendrimers bearing 16 quaternary ammonium groups per molecule were found to exhibit two orders of magnitude greater antimicrobial activity than their mono-functional counterparts [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria

et al. 2020

View full text Add to dashboard Cite

Oxidized multi-walled carbon nanotubes (oxCNTs) were functionalized by a simple non-covalent modification procedure using quaternized hyperbranched poly(ethyleneimine) derivatives (QPEIs), with various quaternization degrees. Structural characterization of these hybrids using a variety of techniques, revealed the successful and homogenous anchoring of QPEIs on the oxCNTs’ surface. Moreover, these hybrids efficiently dispersed in aqueous media, forming dispersions with excellent aqueous stability for over 12 months. Their cytotoxicity effect was investigated on two types of gram(−) bacteria, an autotrophic (cyanobacterium Synechococcus sp. PCC 7942) and a heterotrophic (bacterium Escherichia coli). An enhanced, dose-dependent antibacterial and anti-cyanobacterial activity against both tested organisms was observed, increasing with the quaternization degree. Remarkably, in the photosynthetic bacteria it was shown that the hybrid materials affect their photosynthetic apparatus by selective inhibition of the Photosystem-I electron transport activity. Cytotoxicity studies on a human prostate carcinoma DU145 cell line and 3T3 mouse fibroblasts revealed that all hybrids exhibit high cytocompatibility in the concentration range, in which they also exhibit both high antibacterial and anti-cyanobacterial activity. Thus, QPEI-functionalized oxCNTs can be very attractive candidates as antibacterial and anti-cyanobacterial agents that can be used for potential applications in the disinfection industry, as well as for the control of harmful cyanobacterial blooms.

show abstract

“…G4-PAMAM has a molecular weight of approximately 14,214 g/mol with a size of 4.5 nm, which gives it particular biological properties 10 . G4-PAMAM can cross biological barriers by transcytosis 11 due to the terminal amino groups' positive charges at its surface 12 . Additionally, G4-PAMAM presents a tridimensional structure that can form polar-nonpolar inner cavities due to its internal tertiary amine and methylene groups 13 .…”

Section: Introductionmentioning

confidence: 99%

Chemical characterization (LC–MS–ESI), cytotoxic activity and intracellular localization of PAMAM G4 in leukemia cells

Flores-Mejía

Fragoso-Vázquez

Pérez-Blas

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Generation 4 of polyamidoamine dendrimer (G4-PAMAM) has several biological effects due to its tridimensional globular structure, repetitive branched amides, tertiary amines, and amino-terminal subunit groups liked to a common core. G4-PAMAM is cytotoxic due to its positive charges. However, its cytotoxicity could increase in cancer cells due to the excessive intracellular negative charges in these cells. Furthermore, this work reports G4-PAMAM chemical structural characterization using UHPLC-QTOF-MS/MS (LC–MS) by electrospray ionization to measure its population according to its positive charges. Additionally, the antiproliferative effects and intracellular localization were explored in the HMC-1 and K-562 cell lines by confocal microscopy. The LC–MS results show that G4-PAMAM generated multivalent mass spectrum values, and its protonated terminal amino groups produced numerous positive charges, which allowed us to determine its exact mass despite having a high molecular weight. Additionally, G4-PAMAM showed antiproliferative activity in the HMC-1 tumor cell line after 24 h (IC50 = 16.97 µM), 48 h (IC50 = 7.02 µM) and 72 h (IC50 = 5.98 µM) and in the K-562 cell line after 24 h (IC50 = 15.14 µM), 48 h (IC50 = 14.18 µM) and 72 h (IC50 = 9.91 µM). Finally, our results showed that the G4-PAMAM dendrimers were located in the cytoplasm and nucleus in both tumor cell lines studied.

show abstract

Mechanism of the toxic action of cationic G5 and G7 PAMAM dendrimers in the cyanobacteriumAnabaenasp. PCC7120

Abstract: Cyanobacterial physiology is impaired by high-generation dendrimers.

Cited by 15 publications

References 78 publications

Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii

Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii

Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria

Chemical characterization (LC–MS–ESI), cytotoxic activity and intracellular localization of PAMAM G4 in leukemia cells

Contact Info

Product

Resources

About