1992
DOI: 10.1101/gad.6.12a.2270
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Mechanism of transcriptional antirepression by GAL4-VP16.

Abstract: Promoter-and enhancer-binding factors appear to function by facilitating the transcription reaction as well as by counteracting chromatin-mediated repression (antirepression). We have examined the mechanism by which a hybrid activator, GAL4-VP16, is able to counteract histone HI-mediated repression by using both H1-DNA complexes and reconstituted HI-containing chromatin templates. The GAL4 DNA bind{ng domain alone was sufficient to disrupt local H1-DNA interactions, but a transcriptional activation region was … Show more

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Cited by 96 publications
(66 citation statements)
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“…The mechanism could be attributable to cooperativity in binding of trans-activators. Cooperative binding is not always observed on naked DNA templates and in some cases requires DNA packaged into chromatin (Workman et al 1991;Croston et al 1992;Chang and Gralla 1994). However, synergistic activation has been observed under conditions where the binding sites of the activator are saturated Lin et al 1990}.…”
Section: Transcriptional Synergy Functions At Both Levels Initiationmentioning
confidence: 99%
“…The mechanism could be attributable to cooperativity in binding of trans-activators. Cooperative binding is not always observed on naked DNA templates and in some cases requires DNA packaged into chromatin (Workman et al 1991;Croston et al 1992;Chang and Gralla 1994). However, synergistic activation has been observed under conditions where the binding sites of the activator are saturated Lin et al 1990}.…”
Section: Transcriptional Synergy Functions At Both Levels Initiationmentioning
confidence: 99%
“…For example, nucleosome cores repress basal transcription both in vivo and in vitro, which increases the dependence of promoter function on the action of upstream regulatory factors (16,23,24,34,60,63,64). Similarly, subsequent binding of the linker histone Hi further represses promoter function and increases the dependence of transcription on regulatory factors in vitro (15,30). These studies implicate a crucial role of upstream activators in histone displacement as well as transcription complex formation at the core promoter (i.e., the TATA box and transcription start site).…”
mentioning
confidence: 99%
“…These studies implicate a crucial role of upstream activators in histone displacement as well as transcription complex formation at the core promoter (i.e., the TATA box and transcription start site). This function of upstream activators is dependent on their activation domains (5,15,34,43,64) and is affected in vivo by the stability of nucleosomes located over core promoter sequences (50) and mutations in the N termini of histone H4 (17).…”
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confidence: 99%
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