Mechanism of validamycin A inhibiting DON biosynthesis and synergizing with DMI fungicides against <i>Fusarium graminearum</i>

Bian, Chuanhong; Duan, Yabing; Xiu, Qian; Wang, Jueyu; Tao, Xian; Zhou, Mingguo

doi:10.1111/mpp.13060

Cited by 29 publications

(26 citation statements)

References 67 publications

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“…Beneficial microorganisms include bacteria, actinobacteria, fungi, and viruses, which are closely associated with plant health and safety (Munir et al, 2021 ; Ahmed et al, 2022 ). Microbial metabolites, such as validamycin and streptomycin, are the most widely used biological pesticides at present (Copping and Menn, 2000 ; Bian et al, 2021 ). Trichoderma was first isolated from soil in 1794 and it has been a well-known biocontrol resource since then.…”

Section: Introductionmentioning

confidence: 99%

Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

Liu

He²,

Munir³

et al. 2022

Front. Microbiol.

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Plant health is of utmost importance for optimal agricultural production and sustainability. Unfortunately, biotic and abiotic factors put a major constraint on crop safety and productivity. Plant diseases caused by oomycetes inflict serious damage to various crops. Moreover, the injudicious use of chemical pesticides poses threats related to pesticide resistance development in pathogens and environmental pollution. Biocontrol offers an effective solution for disease control; however, research on biocontrol of oomycete-related diseases is scarce. Thus, this study undertakes the screening of biocontrol resources for the effective management of oomycete-related plant diseases. In this regard, 86 isolates of Trichoderma spp. were assessed against Phytophthora nicotianae, P. capsici, Pythium vexans, P. ultimum, and P. dissotocum through dual culture assay. Furthermore, the antagonistic effect of selected isolates was studied against tobacco black shank disease and damping-off of cucumber seedlings in the greenhouse. The relative control effect of the three antagonistic Trichoderma strains AR-4, Tv-1, and ST4-1 on tobacco black shank was more than 60%, which was not significantly different from 6.88 gl−1 fluopicolide–propamocarb. Whereas, the relative control effect of Trichoderma AR-4 and ST4-1 on damping-off of cucumber seedlings was 80.33% and 82.67%, respectively, which were significantly higher than Trichoderma Tv-1 (35.49%) and fluopicolide–propamocarb (47.82%). According to the morphological and molecular characterization, the fungal strains AR-4, Tv-1, and ST4-1 were identified as Trichoderma koningiopsis, T. asperellum, and T. gamsii, respectively. In conclusion, the strains exhibited a strong antagonistic effect against oomycete pathogens and can be integrated into disease management strategies.

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Section: Introductionmentioning

confidence: 99%

Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

Liu

He²,

Munir³

et al. 2022

Front. Microbiol.

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“…Streptomycin is a broad-spectrum drug effective against both Gram-negative aerobic bacteria and staphylococci. Another aminoglycoside compound is validamycin [ 52 ], which has been used to control the rice sheath blight caused by Rhizoctonia solani for over 50 years in China, with no validamycin-resistant isolates reported in the field [ 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Deep-Sea Sediments from the Southern Gulf of Mexico Harbor a Wide Diversity of PKS I Genes

et al. 2022

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The excessive use of antibiotics has triggered the appearance of new resistant strains, which is why great interest has been taken in the search for new bioactive compounds capable of overcoming this emergency in recent years. Massive sequencing tools have enabled the detection of new microorganisms that cannot be cultured in a laboratory, thus opening the door to the search for new biosynthetic genes. The great variety in oceanic environments in terms of pressure, salinity, temperature, and nutrients enables marine microorganisms to develop unique biochemical and physiological properties for their survival, enhancing the production of secondary metabolites that can vary from those produced by terrestrial microorganisms. We performed a search for type I PKS genes in metagenomes obtained from the marine sediments of the deep waters of the Gulf of Mexico using Hidden Markov Models. More than 2000 candidate genes were detected in the metagenomes that code for type I PKS domains, while biosynthetic pathways that may code for other secondary metabolites were also detected. Our research demonstrates the great potential use of the marine sediments of the Gulf of Mexico for identifying genes that code for new secondary metabolites.

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“…In the current study, FGSG_01959 encoding a cytochrome P450 protein associated with farnesyl pyrophosphate in F. graminearum, was signi cantly up-regulated with phenamacril treatment. Farnesyl pyrophosphate (FPP) converted from acetyl-CoA from the isoprenoid biosynthetic pathway, is the immediate precursor of DON biosynthesis [53]. FgCYP51B (FGSG_01000), encoding cytochrome P450-51 protein, and FGSG_09786 encoding NADPH-cytochrome P450 reductase, were downregulated with carbendazim treatment.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis reveals distinct gene expression profiles in Fusarium graminearum challenged with carbendazim and phenamacril

Guo

Jiang

Qian

et al. 2022

Preprint

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Background: Fusarium graminearum, which causes Fusarium head blight (FHB) on cereal crops worldwide, is an economically important phytopathogenic fungus. Chemical control is the dominant method to manage FHB. Results: In this study, the sensitivity of F. graminearum against carbendazim and phenamacril of two commonly-used fungicides with different mode of action weredetermined. A total of 5086 and 2559 differentially expressed genes (DEGs) were identified in F. graminearum with carbendazim and phenamacril treatment, respectively, by RNA-seq. Gene Ontology (GO) enrichment analysis showed proteasome complex, transporter activity, and transmembrane transporter activity were most enriched with carbendazim treatment, whereas ion binding, ribonucleotide binding and carbohydrate derivative binding were most enriched with phenamacril treatment. The pathway enrichment analysis demonstrated proteasome, ribosome biogenesis in eukaryotes and pentose phosphate pathway were associated with carbendazim response while nitrogen metabolism, glutathione metabolism and citrate cycle (TCA cycle) were associated with phenamacril response. Furthermore, protein-protein interaction (PPI) network analysis was performed to elucidate protein and metabolic networks in F. graminearum response of fungicide. In addition, the results show that those genes associated with ATP-binding cassette transporters, heat shock proteins and stress response were changed expression and genes regulating trichothecenes biosynthesis were altered with two fungicide treatment. Conclusions: Taken together, those results promoted to unravel the action mechanism of carbendazim and phenamacril on F. graminearum and provide valuable resources for searching novel antifungal in the future to improve strategies managing FHB.

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Mechanism of validamycin A inhibiting DON biosynthesis and synergizing with DMI fungicides against Fusarium graminearum

Cited by 29 publications

References 67 publications

Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

Deep-Sea Sediments from the Southern Gulf of Mexico Harbor a Wide Diversity of PKS I Genes

Comparative transcriptome analysis reveals distinct gene expression profiles in Fusarium graminearum challenged with carbendazim and phenamacril

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