Mechanism of Yangxinshi Intervention on Cardiac Fibrosis in Diabetic Cardiomyopathy Based on Network Pharmacology

Abstract

Background. Cardiac fibrosis (CF) is major myocardial change in diabetic cardiomyopathy (DCM). Yangxinshi as a Chinese medicine formula is used to treat cardiovascular diseases. However, the exact effective mechanism of Yangxinshi on CF is still uncertain. Hence, based on the pharmacological network, predicting the active components, potential targets and pathways of Yangxinshi on diabetic fibrosis require to be further studied. Materials and Methods. By using Cytoscape 3.6.0 Bisogenet plug-in, the active components of Yangxinshi were obtained and screened through TCMSP, and the PPI network of DCM-CF was constructed and then screened by CytoNCA plug-in. GO analysis and KEGG pathway enrichment analysis were carried out by Cluego plug-in. Combined with the results of network pharmacological analysis, cells in vitro were performed to verify the CF stimulated with high glucose or intervence with Yangxinshi, and the expressions of Cbl-b, p-smad2, and α-SMA were detected. Results. Yangxinshi might play a key role in reversing cardiac fibrosis in individuals with DCM by regulating the signal pathway of CBL and promoted the expression of Cbl-b and inhibited the expression of p-smad2 and α-SMA, verifying some predictive work via network pharmacology. Conclusion. Based on network pharmacology, this study demonstrates that the beneficial effect of Yangxinshi on CF is related to the Cbl-b/smad2 pathway, providing an idea for the therapeutic effect of Yangxinshi on cardiac fibrosis in DCM.

Online preconcentration and determination of five alkaloids in Yangxinshi tablet by large‐volume sample stacking and micelle to solvent stacking in cyclodextrin‐modified electrokinetic chromatography

“…Cardiovascular disease (CVD) is a serious threat to human health with high mortality and morbidity (Almazán‐Ávila, 2020; Boateng et al, 2017; Kalayinia et al, 2021). Clinical practices have proved that Yangxinshi tablet (YXST) is safe and effective for CVD such as coronary heart disease, angina pectoris, myocardial infarction, hyperlipidemia and hyperglycemia (Kuang et al, 2022; Wu et al, 2020; Zhang et al, 2020). As a traditional Chinese medicine preparation, YXST consists of 13 Chinese herbs: Astragali Radix, Ginseng Radix et Rhizoma, Codonopsis Radix, Salviae Miltiorrhizae Radix et Rhizoma, Angelicae Sinensis Radix, Puerariae Lobatae Radix, Epimedii Folium, Crataegi Fructus, Rehmanniae Radix, Coptidis Rhizoma, Corydalis Rhizoma, Ganoderma and Glycyrrhizae Radix et Rhizoma (Zhang et al, 2018).…”

LncRNA TUG1 Exacerbates Myocardial Fibrosis in Diabetic Cardiomyopathy by Modulating the microRNA-145a-5p/Cfl2 Axis

“…Diabetic cardiomyopathy (DCM) is a prominent cardiovascular complication of diabetes. 1 The main features of DCM include myocardial fibrosis, myocardial hypertrophy, and cardiac dysfunction, which ultimately lead to heart failure. 2 It is reported that inflammation, mitochondrial dysfunction, oxidative stress, cell autophagy, and metabolic disturbance play a role in the pathogenesis of DCM, and there is a complicated mechanism underlying the process of DCM.…”

Network pharmacology integrated with molecular docking technology to reveal the potential mechanism of Shuganfang against drug-induced liver injury

“…In recent years, network pharmacology has emerged as a valuable tool for investigating the intricate mechanisms underlying the efficacy of TCM in complex systems. [14][15][16] In this particular study, network pharmacology and molecular docking analysis were utilized to predict the bioactive ingredients, potential molecular targets, and signaling pathways associated with the hepatoprotective effects of SGF against DILI. The results obtained from this research significantly contribute to a more comprehensive comprehension of the pharmacodynamics and mechanisms of action of SGF in the management of DILI.…”

“…In recent years, network pharmacology has emerged as a valuable tool for investigating the intricate mechanisms underlying the efficacy of TCM in complex systems. [14–16]…”