Mechanism Study of Bacteria Killed on Nanostructures

Abstract

It is important to study the bactericidal mechanism with nanostructures for the design and preparation of high-efficiency sterilization materials. In this paper, the interfacial energy gradient between cells and nanopillars is proposed to be the driving force to promote cells to migrate into nanostructures and get killed. The expressions of interfacial energy and its gradient were first established, then the deformation of cells pressured by nanostructures was calculated. The results show that the interfacial energy gradient or the pressure on cells is influenced by nanopillar parameters substantially. The smaller the nanopillar diameter and the larger the pitch, the greater the pressure on cells. Only high enough nanocolumns can ensure sufficient cell creep deformation and become punctured. Furthermore, a cell volume and its adhesion morphology also influence the interaction between cells and nanostructures. The smaller the cell volume, the greater the pressure on it. And the larger the contact angle of adhered cells, the greater the pressure on the cells by nanopillars. Besides, the wettability of substrate material also influences the interaction between cells and nanopillars. It can be concluded that the model is reasonable and reliable since its calculation results are in good accordance with the experimental measurements.

Bioinspired Multifunctional Glass Surfaces through Regenerative Secondary Mask Lithography

“…[ 46 ] Here, we focus on the interactions with S. aureus (Gram‐positive—highly rigid and thus harder to inactivate than Gram‐negative species), [ 46 ] and for guidance, calculate the pressure exerted on cells by various topographies (Figures S21 and S22, Supporting Information). [ 47 ] The data confirm a lower tip diameter generates higher pressure exerted on the cell, with pitch being inversely proportional (Figure S22, Supporting Information). For our P100 structures, ( p = 110 nm; d T = 21 nm), the model predicts a pressure of ≈10 MPa indicating that creep deformation can occur with the potential to rupture given sufficient nanopillar height.…”

Biomimetic Functional Surfaces towards Bactericidal Soft Contact Lenses

“…Furthermore, the model indicated that high-motile bacteria are less resistant to nanopatterned surfaces [57]. Liu et al [58] implemented an investigation into the interfacial energy gradient between the cells and nanopillars, which was proposed as the driving force to promote cell adhesion, and the results implied that nanopillar parameters were a substantial influence factor of the interfacial energy gradient. Higher aspect ratio could exert greater pressure on the cell membrane, while smaller cell volume would be exerted at higher pressure with larger contact angle.…”

Resolving Bio–Nano Interactions of E. coli Bacteria–Dragonfly Wing Interface with Helium Ion and 3D-Structured Illumination Microscopy to Understand Bacterial Death on Nanotopography

“…The investigation of bacteria-nanotopography interactions has, therefore, become a significant research interest for the efficient and effective development of advanced fabricated bactericidal nanotextured surfaces (NTS) for biomaterials application. [5][6][7][8] This is a growing field of research as nanotextured surfaces do not leach bactericidal chemicals, and therefore, the bactericidal property is conserved for longer periods compared to chemical approaches. [9][10] Furthermore, nanotextured surfaces are non-toxic and more effective at controlling bacterial strains that produce extracellular polymeric substance (EPS) secretions.…”