A novel,
highly biocompatible bacterial cellulose (BC)-zein composite
nanofiber with a controlled hydrophobic biosurface was successfully
developed through a simple and green solution impregnation method,
followed by evaporation-induced self-assembly (EISA) of adsorbed zein
protein. The surface hydrophobicity of the zein-modified BC nanofibers
could be controlled by simply changing the zein concentration, which
is able to tune the morphology of self-assembled zein structures after
EISA, thus affecting the surface roughness of composite membranes.
Zein self-assembly at low concentrations (5 mg/mL) resulted in the
formation of hierarchical zein structures (spheres and bicontinuous
sponges) on the BC surface, thus increasing the surface roughness
and leading to high hydrophobicity (the water contact angle reached
110.5°). However, at high zein concentrations, these large zein
spheres assembled into a flat zein film, which decreased the surface
roughness and hydrophobicity of membranes. The homogeneous incorporation
of zein structures on the BC surface by hydrogen bonding did not significantly
change the internal structure and mechanical performance of BC nanofibers.
In comparison with pure BC, the BC-zein nanofibers had a better biocompatibility,
showing a significantly increased adhesion and proliferation of fibroblast
cells. This is probably due to the rough surface structure of BC-zein
nanofibers as well as the high biocompatibility of natural zein protein.
The novel BC-zein composite nanofibers with controlled surface roughness
and hydrophobicity could be of particular interest for the design
of BC-based biomaterials and biodevices that require specific surface
properties and adhesion.