Mechanism underlying retinoic acid-dependent metamorphosis in the starfish

Yamakawa, Shumpei; Hayashi, Yoshiki; Kako, Koichiro; Sasakura, Yasunori; Morino, Yoshiaki; Wada, Hiroshi

doi:10.1016/j.ydbio.2022.10.002

Cited by 1 publication

(12 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent to our previous reports showing a high efficiency of TALEN in P. pectinifera. 10,23 Regarding that gene function analysis is conducted at F0 generation in starfish due to their long life cycles, the efficiency of mutagenesis is of vast importance. 31 Future studies should quantify and confirm the mutagenesis efficiencies of each technique in multiple genes/targets within the same species.…”

Section: Discussionmentioning

confidence: 99%

“…20,21 Recently, genome editing techniques have been also employed in studies using starfish. 10,13,[22][23][24][25] For example, we previously reported the feasibility of the genome editing method TALEN in the starfish Patiria pectinifera. 10,23 In addition, the CRISPR/Cas9-based genome editing has also been achieved in P. miniata.…”

Section: Introductionmentioning

confidence: 99%

“…10,13,[22][23][24][25] For example, we previously reported the feasibility of the genome editing method TALEN in the starfish Patiria pectinifera. 10,23 In addition, the CRISPR/Cas9-based genome editing has also been achieved in P. miniata. 22,24,25 These methods will pave the way for new experimental options and strategies to study the molecular development of starfish embryos and larvae.…”

Section: Introductionmentioning

confidence: 99%

“…25 On the other hand, we previously amplified and cloned a total of 46 DNA fragments from the genomes of TALEN knockout P. pectinifera embryos, and the sequencing analysis showed that mutagenesis was observed in all fragments. 10,23 Based on these advantages of TALEN, it is important to establish a robust assay of the genome editing with not only CRISPR/Cas9 but TALEN in starfish.…”

Section: Introductionmentioning

confidence: 99%

“…10 Yamakawa et al also examined the function of rar (retinoic acid receptor) and found that targeted mutagenesis occurred until 3 dpf. 23 Despite the feasibility of TALEN in P. pectinifera, when TALEN causes gene cleavage is still unknown. Embryonic development proceeds until approximately 48 hpf (hours postfertilization) in this species at 22 C; the cleavage (morula), blastula, and gastrula stages are observed at $6, 12, and 24 hpf in this species, respectively.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Detection of TALEN‐mediated genome cleavage during the early embryonic stage of the starfish Patiria pectinifera

Yamakawa

Sasakura

Morino

et al. 2023

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

BackgroundEchinoderms have long been utilized as experimental materials to study the genetic control of developmental processes and their evolution. Among echinoderms, the molecular study of starfish embryos has received considerable attention across research topics such as gene regulatory network evolution and larval regeneration. Recently, experimental techniques to manipulate gene functions have been gradually established in starfish as the feasibility of genome editing methods was reported. However, it is still unclear when these techniques cause genome cleavage during the development of starfish, which is critical to understand the timeframe and applicability of the experiment during early development of starfish.ResultsWe herein reported that gene functions can be analyzed by the genome editing method TALEN in early embryos, such as the blastula of the starfish Patiria pectinifera. We injected the mRNA of TALEN targeting rar, which was previously constructed, into eggs of P. pectinifera and examined the efficiency of genome cleavage through developmental stages from 6 to 48 hours post fertilization.ConclusionThe results will be key knowledge not only when designing TALEN‐based experiments but also when assessing the results.

show abstract

Section: Discussionmentioning

confidence: 99%