Mechanism whereby nitric oxide (NO) infused chronically intrauterine in ewes is antiluteolytic rather than being luteolytic

Weems, Y.S.; Lennon, E.; Uchima, T.; Raney, A.; Goto, K.; Ong, A.; Zaleski, Halina M.; Weems, C.W.

doi:10.1016/j.prostaglandins.2007.10.003

Cited by 14 publications

(5 citation statements)

References 107 publications

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“…NONOate) only stimulated PGRB mRNA expression on Days 6-10 of the oestrous cycle. These data are supported by those of Weems et al (2008), who reported that intrauterine administration of an NO donor in ewes prevented the decline in P4 secretion, whereas an NO synthase inhibitor did not affect luteolysis. NO activity also altered the PGE : PGF 2a ratio, most likely by inhibiting 9-keto-PGE 2 reductase-mediated conversion of PGE 2 to PGF 2a (Weems et al 2008) .…”

Section: Table 2 Summary Of Changes In the Mrna Expression Of Progessupporting

confidence: 82%

“…These data are supported by those of Weems et al (2008), who reported that intrauterine administration of an NO donor in ewes prevented the decline in P4 secretion, whereas an NO synthase inhibitor did not affect luteolysis. NO activity also altered the PGE : PGF 2a ratio, most likely by inhibiting 9-keto-PGE 2 reductase-mediated conversion of PGE 2 to PGF 2a (Weems et al 2008) . Thus, we assume that NO strengthens the impact of P4 in the cow uterus through stimulation of PGRB mRNA only, which indicates its luteotropic rather than luteolytic action.…”

Section: Table 2 Summary Of Changes In the Mrna Expression Of Progessupporting

confidence: 82%

“…NO is a strong blood flow pressure regulator (Cameron and Campbell 1998), but it also affects the secretion of both PGE 2 and PGF 2a (Woclawek-Potocka et al 2004). In addition, NO exerts antiluteolytic properties by inhibiting the 9-keto-PGE 2 reductase, which converts PGE 2 to PGF 2a , as demonstrated in sheep endometrium (Weems et al 2008).…”

Section: Introductionmentioning

confidence: 98%

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Luteotropic and luteolytic factors regulate mRNA and protein expression of progesterone receptor isoforms A and B in the bovine endometrium

Rękawiecki

Kowalik

Kotwica

2016

Reprod. Fertil. Dev.

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The aim of the present study was to examine the effects of luteotropic and luteolytic factors on the mRNA and protein levels of progesterone receptor isoforms A (PGRA) and B (PGRB) in the bovine endometrium. Endometrial slices from Days 6-10 and 17-20 of the oestrous cycle were treated with LH (100ngmL), oestradiol (E2; 1×10M), prostaglandin (PG) E (1×10M) and PGF (1×10M) and the nitric oxide donor NONOate (1×10M); these treatments lasted for 6h for mRNA expression analysis and 24h for protein expression analysis. On Days 6-10 of the oestrous cycle PGRAB (PGRAB; the entire PGRA mRNA sequence is common to the PGRB mRNA sequence) mRNA expression in endometrial slices was enhanced by E2 treatment (P<0.001), whereas PGRB mRNA expression was increased by LH (P<0.001), E2 (P<0.05) and NONOate (P<0.05) treatment. On Days 17-20, PGRAB mRNA expression increased after E2 (P<0.001) and PGE (P<0.05) treatment; PGRB mRNA expression was increased by PGE (P<0.05) and PGF (P<0.01) treatment, but decreased by LH (P<0.05). On Days 6-10 protein levels of PGRA were stimulated by E2 (P<0.01), whereas PGRB protein levels were increased by LH (P<0.05) and E2 (P<0.05). On Days 17-20 of the oestrous cycle, PGRA protein levels were enhanced by E2 (P<0.05) and PGF (P<0.05), whereas PGRB protein levels were stimulated by PGE (P<0.05) and PGF (P<0.001). These data suggest that luteotropic and luteolytic factors affect PGRA and PGRB mRNA and protein levels, and this may regulate the effects of progesterone on endometrial cells.

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Section: Table 2 Summary Of Changes In the Mrna Expression Of Progessupporting

confidence: 82%

Section: Table 2 Summary Of Changes In the Mrna Expression Of Progessupporting

confidence: 82%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Luteotropic and luteolytic factors regulate mRNA and protein expression of progesterone receptor isoforms A and B in the bovine endometrium

Rękawiecki

Kowalik

Kotwica

2016

Reprod. Fertil. Dev.

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“…254 Responses of NO appeared to differ in regard to functional and structural regression. 272 That interpretation could fit with the conclusion that decreased NO contributed to structural luteal regression. 249 In contrast to reports that NO is luteolytic, uterine infusion of NO donors during days 8-18 of the ovine estrous cycle produced heavier corpora lutea, greater progesterone in the jugular vein, and a greater ratio of PGE 2 :PGF 2α in the inferior vena cava in ewes that received the NO donor.…”

Section: Physiological Control Systems and Governing Gonadal Functionmentioning

confidence: 81%

Control of the Ovarian Cycle of the Sheep

Goodman

Inskeep

2015

Knobil and Neill's Physiology of Reproduction

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“…Nitric oxide donor (2S)-2-pentylbutanedioic acid (NONate) is a compound that regulates blood flow and stimulates PGF2α synthesis in a cow's CL [21]. It also exerts anti-luteolytic properties by inhibiting the 9-keto-PGE2 reductase, which converts PGE2 to PGF2a, as demonstrated in sheep endometrium [22]. Aminoglutethimide (AMG) inhibits enzymes such as the cholesterol side-chain cleavage enzyme (CYP11A1, P450scc) and aromatase (CYP19A1), thus inhibiting the entire process of steroidogenesis [23].…”

Section: Introductionmentioning

confidence: 99%

Luteotropic and Luteolytic Factors Modulate the Expression of Nuclear Receptor Coregulators in Bovine Luteal Cells Independently of Histone Acetyltransferase and Histone Deacetylase Activities

Rekawiecki,

Wrobel,

Zajac

et al. 2023

Animals

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The aims of this study were to examine the effect of luteotropic and luteolytic factors on the mRNA and protein expression of the coactivators HAT: histone acetyltransferase p300 (P300), cyclic adenosine monophosphate response element-binding protein (CREB), and steroid receptor coactivator-1 (SRC-1) and the corepressor: nuclear receptor corepressor-2 (NCOR-2) in bovine luteal cells on days 6–10 and 16–20. HAT and HDAC activities were also measured. The obtained results showed that luteotropic and luteolytic factors influence changes in the mRNA and protein levels of the coregulators of PGRs. However, they did not affect the activity of related HAT and HDAC, respectively. Therefore, it is possible that these factors, through changes in the expression of nuclear receptor coactivators and corepressors, may affect the functioning of the nuclear receptors, including PGRs, in the bovine CL.

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Mechanism whereby nitric oxide (NO) infused chronically intrauterine in ewes is antiluteolytic rather than being luteolytic

Cited by 14 publications

References 107 publications

Luteotropic and luteolytic factors regulate mRNA and protein expression of progesterone receptor isoforms A and B in the bovine endometrium

Luteotropic and luteolytic factors regulate mRNA and protein expression of progesterone receptor isoforms A and B in the bovine endometrium

Control of the Ovarian Cycle of the Sheep

Luteotropic and Luteolytic Factors Modulate the Expression of Nuclear Receptor Coregulators in Bovine Luteal Cells Independently of Histone Acetyltransferase and Histone Deacetylase Activities

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