Mechanisms and biotechnological applications of transcription factors

He, Hehe; Yang, Mingfei; Li, Siyu; Zhang, Gaoyang; Ding, Zhongyang; Zhang, Liang; Shi, Guiyang; Li, Youran

doi:10.1016/j.synbio.2023.08.006

Cited by 19 publications

(6 citation statements)

References 117 publications

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“…Several methods have been developed to identify TFBSs in microalgal genomes. Experimental methods, such as electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation, provide valuable insights into the direct interactions between transcription factors and their DNA binding sites ( Galas and Schmitz, 1978 ; Garner and Revzin, 1981 ; Jothi et al, 2008 ; He et al, 2023 ). EMSAs involve incubating transcription factors with labeled DNA fragments containing potential TFBSs.…”

Section: Transcription Factors-based Metabolic Engineeringmentioning

confidence: 99%

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Harnessing genetic engineering to drive economic bioproduct production in algae

Gupta,

Kang,

Pathania

et al. 2024

Front. Bioeng. Biotechnol.

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Our reliance on agriculture for sustenance, healthcare, and resources has been essential since the dawn of civilization. However, traditional agricultural practices are no longer adequate to meet the demands of a burgeoning population amidst climate-driven agricultural challenges. Microalgae emerge as a beacon of hope, offering a sustainable and renewable source of food, animal feed, and energy. Their rapid growth rates, adaptability to non-arable land and non-potable water, and diverse bioproduct range, encompassing biofuels and nutraceuticals, position them as a cornerstone of future resource management. Furthermore, microalgae’s ability to capture carbon aligns with environmental conservation goals. While microalgae offers significant benefits, obstacles in cost-effective biomass production persist, which curtails broader application. This review examines microalgae compared to other host platforms, highlighting current innovative approaches aimed at overcoming existing barriers. These approaches include a range of techniques, from gene editing, synthetic promoters, and mutagenesis to selective breeding and metabolic engineering through transcription factors.

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Section: Transcription Factors-based Metabolic Engineeringmentioning

confidence: 99%

“…Frontiers in Bioengineering and Biotechnology frontiersin.org Schmitz, 1978;Garner and Revzin, 1981;Jothi et al, 2008;He et al, 2023). EMSAs involve incubating transcription factors with labeled DNA fragments containing potential TFBSs.…”

Section: Transcription Factors Binding Sitesmentioning

confidence: 99%

Harnessing genetic engineering to drive economic bioproduct production in algae

Gupta,

Kang,

Pathania

et al. 2024

Front. Bioeng. Biotechnol.

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“…Transcription factors (TFs) are key proteins that control the fine-tuning expression of target genes by activating or suppressing gene transcription in a variety of biological processes (He et al 2023). Cells have evolved to optimize cellular function through the coordinated regulation of multiple enzymes and pathways by different transcription factors in response to different environmental conditions.…”

Section: Transcription Factor Engineeringmentioning

confidence: 99%

Strategies to increase the robustness of microbial cell factories

Xu,

Lin,

Zhang

et al. 2024

Adv. Biotechnol.

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Engineering microbial cell factories have achieved much progress in producing fuels, natural products and bulk chemicals. However, in industrial fermentation, microbial cells often face various predictable and stochastic disturbances resulting from intermediate metabolites or end product toxicity, metabolic burden and harsh environment. These perturbances can potentially decrease productivity and titer. Therefore, strain robustness is essential to ensure reliable and sustainable production efficiency. In this review, the current strategies to improve host robustness were summarized, including knowledge-based engineering approaches, such as transcription factors, membrane/transporters and stress proteins, and the traditional adaptive laboratory evolution based on natural selection. Computation-assisted (e.g. GEMs, deep learning and machine learning) design of robust industrial hosts was also introduced. Furthermore, the challenges and future perspectives on engineering microbial host robustness are proposed to promote the development of green, efficient and sustainable biomanufacturers.

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“…In this study, after spraying melatonin, the proline content, soluble sugar, and soluble protein content of Clematis were enhanced, thus effectively maintaining cell homeostasis and improving the adaptability of Clematis to waterlogging stress. Transcription factors (TFs) are key regulators of gene expression, and they play a crucial role in regulating plant responses to various abiotic stresses (He et al, 2023). C. lanuginosa and C. tientaiensis are the original breeding parents of Clematis.…”

Section: Melatonin Maintained the Chlorophyll Content In Clematis Undermentioning

confidence: 99%

The effect of exogenous melatonin on waterlogging stress in Clematis

Chen,

Hu,

et al. 2024

Front. Plant Sci.

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Clematis is the queen of the vines, being an ornamental plant with high economic value. Waterlogging stress reduces the ornamental value of the plant and limits its application. Melatonin plays an important role in plant resistance to abiotic stresses. In this study, the physiological responses and gene expression levels of two wild species, namely, Clematis tientaiensis and Clematis lanuginosa, and two horticultural varieties, namely, ‘Sen-No-Kaze’ and ‘Viva Polonia,’ under waterlogging stress were analyzed to determine the effect of melatonin on waterlogging tolerance. The results showed that the waterlogging tolerances of C. lanuginosa and ‘Sen-No-Kaze’ were relatively poor, but were significantly improved by concentrations of 100 μmol·L-1 and 50 μmol·L-1 melatonin. C. tientaiensis and ‘Viva Polonia’ had relatively strong tolerance to waterlogging, and this was significantly improved by 200 μmol·L-1 melatonin. Under waterlogging stress, the relative conductivity and H2O2 content of Clematis increased significantly; the photosynthetic parameters and chlorophyll contents were significantly decreased; photosynthesis was inhibited; the contents of soluble protein and soluble sugars were decreased. Effective improvement of waterlogging tolerance after exogenous melatonin spraying, the relative conductivity was decreased by 4.05%-27.44%; the H2O2 content was decreased by 3.84%-23.28%; the chlorophyll content was increased by 35.59%-103.36%; the photosynthetic efficiency was increased by 25.42%-45.86%; the antioxidant enzyme activities of APX, POD, SOD, and CAT were increased by 28.03%-158.61%; the contents of proline, soluble protein, and soluble sugars were enhanced, and cell homeostasis was improved. Transcription sequencing was performed on wild Clematis with differences in waterlogging tolerance, and nine transcription factors were selected that were highly correlated with melatonin and that had the potential to improve waterlogging tolerance, among which LBD4, and MYB4 were significantly positively correlated with the antioxidant enzyme system, and bHLH36, DOF36, and WRKY4 were significantly negatively correlated. Photosynthetic capacity was positively correlated with DOF36 and WRKY4 while being significantly negatively correlated with MYB4, MOF1, DOF47, REV1 and ABR1. Melatonin could enhance the flooding tolerance of Clematis by improving photosynthetic efficiency and antioxidant enzyme activity. This study provides an important basis and reference for the application of melatonin in waterlogging-resistant breeding of Clematis.

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Mechanisms and biotechnological applications of transcription factors

Cited by 19 publications

References 117 publications

Harnessing genetic engineering to drive economic bioproduct production in algae

Harnessing genetic engineering to drive economic bioproduct production in algae

Strategies to increase the robustness of microbial cell factories

The effect of exogenous melatonin on waterlogging stress in Clematis

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