Mechanisms and Consequences of Neutrophil Interaction with the Subgingival Microbiota

Dyke, Thomas E. Van; Duncan, Robert L.; Cutler, Chelsea; Kalmar, John R.; Arnold, Roland R.

doi:10.1159/000416657

Cited by 11 publications

(12 citation statements)

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“…In contrast to the findings reported here. Cutler et al {1991b) have shown that serum from !8 patients with AP and with high IgG antibody levels to R gingivalis opsonises poorly for PMN phagocytosis, extending and confirming the results of an earlier study in one patient (Van Dyke et al 1988). Their assays (Cutler et al, 1991b) used iive bacteria and their P, gingivalis strain (A 7346) was also different from that used in our study.…”

Section: Discussionsupporting

confidence: 88%

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Elevated opsonic activity for Porphyromonas (Bacteroides) gingivalis in serum from patients with a history of destructive periodontal disease

Wilton¹,

Hurst²,

Sterne³

1993

J Clinic Periodontology

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We have measured the opsonic capacity of serum for the phagocytosis of Porphyromonas (Bacteroides) gingivalis by polymorphonuclear leucocytes (PMN) in 35 patients with a history of destructive periodontitis and 35 matched control subjects. The serum from cases, tested at concentrations of 8% and 0.8% opsonised P. gingivalis for phagocytosis by PMN to a level significantly greater than controls (p < 0.0001 and < 0.01 respectively). IgG antibody levels to P. gingivalis whole cells estimated by ELISA were also significantly higher in the cases (p < 0.0001). The IgG antibody levels correlated significantly with the opsonic capacity of the serum tested at 8% concentration in controls (r = 0.371, p = 0.03) but not in cases (r = 0.235, p = 0.17); in 0.8% serum, the opsonic capacity of the cases and controls were not significantly correlated. Elevated opsonisation by serum was a significant predictor that a subject was a case rather than a control, even after allowing for the effect of elevated IgG antibody in the cases. The data suggest that an elevated capacity of serum to opsonise P. gingivalis is a distinctive feature in patients with past destructive periodontal disease.

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Section: Discussionsupporting

confidence: 88%

“…P. gingivalis, opsonised with pooled normal human serum were also phagocytosed by human PMN and induced a chemi luminescent response in these celis (Sundqvist et al 1982), In contrast. Van Dyke et al (1988) showed that the phagocytosis of two strains of P. gingivalis. opsonised with serum from heaithy controls or a patient with AP, by human PMN was very low.…”

mentioning

confidence: 99%

Elevated opsonic activity for Porphyromonas (Bacteroides) gingivalis in serum from patients with a history of destructive periodontal disease

Wilton¹,

Hurst²,

Sterne³

1993

J Clinic Periodontology

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“…An aliquot of cell suspension was diluted and subcultured onto ETSA plates and gram‐stained to verify the culture purity of P. gingivalis . Previous studies with vital (propidium iodide) staining (29, 54) demonstrated that >95% of bacteria treated in this way are viable at the time of infection. Mice were infected within 15–30 min of bacterial preparation.…”

Section: Methodsmentioning

confidence: 99%

In vitro environmental regulation of Porphyromonas gingivalis growth and virulence

Kesavalu

Holt²,

Ebersole

2003

Oral Microbiology and Immunology

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Porphyromonas gingivalis appears to be a major contributor to periodontal disease, especially soft tissue destruction, which is reflected by the ability to cause invasive, spreading lesions, and tissue inflammation in a murine abscess model. This study investigated the role of hemin on the regulation of growth and virulence of P. gingivalis strains. P. gingivalis strains W50, A7A1-28, 3079, 381, W50/BEI, and NG4B19 were grown in broth and on blood agar plates. P. gingivalis cells grown under iron-depleted conditions for multiple passages showed significantly decreased lesion size in mice, in contrast to cells grown under iron-normal (5 microg/ml) and iron-elevated conditions. Statistically significant (P < 0.01) decreases in gingipain enzyme activity were found among the strains grown under iron-depleted conditions. P. gingivalis grown in the presence of blood induced significantly different lesion type, lesion size, lesion onset, and mortality. Elevated hemin resulted in increased cell-associated iron in P. gingivalis, which increased the capacity of the microorganism to survive at times of iron deprivation. These results indicate that hemin or iron availability regulates multiple aspects related to P. gingivalis virulence, including growth, survival, gingipain levels, and iron accumulation.

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“…Mice were infected within 15–30 min of bacterial preparation. Previous studies with vital (propidium iodide) staining (17, 30) demonstrated that > 95% of bacteria treated in this way are viable at the time of infection.…”

Section: Methodsmentioning

confidence: 99%

In vivo induction of proinflammatory cytokines in mouse tissue by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans

Kesavalu

Chandrasekar

Ebersole

2002

Oral Microbiology and Immunology

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Periodontitis is a chronic inflammatory disease initiated by a multitude of bacteria. Persistent infection leads to generation of various inflammatory mediators, resulting in tissue destruction and osteoclastic resorption of the alveolar bone. This study describes a novel in vivo murine calvarial model to assess the effects of oral pathogens on the expression of three proinflammatory cytokines [interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha] which are involved in bone resorption. We chose Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans as prototype oral pathogens. We also tested the effects of Streptococcus gordonii, an oral commensal supragingival microorganism, considered a non-pathogen. Live bacteria were injected into subcutaneous tissue overlying the parietal bone of mice calvaria for 6 days. At the end of the experimental period, tissues overlying the calvaria were removed and analyzed for proinflammatory cytokine expression by Northern blotting. Cytokine mRNA was not detected in the tissue over the calvaria of control animals. In contrast, P. gingivalis and A. actinomycetemcomitans elicited mRNA expression of all three cytokines, TNFalpha being the highest (TNFalpha > > IL-1beta > IL-6). P. gingivalis was more potent than A. actinomycetemcomitans in inducing cytokine expression. In contrast, S. gordonii induced only low levels of mRNA for IL-1beta and TNFalpha but no IL-6 mRNA induction. These results suggest that oral microorganisms with access to host tissues elicit a battery of proinflammatory cytokines. There were clear differences in profiles and, interestingly, a commensal bacterium also stimulated bone resorptive cytokine expression in host tissues.

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Mechanisms and Consequences of Neutrophil Interaction with the Subgingival Microbiota

Cited by 11 publications

References 0 publications

Elevated opsonic activity for Porphyromonas (Bacteroides) gingivalis in serum from patients with a history of destructive periodontal disease

Elevated opsonic activity for Porphyromonas (Bacteroides) gingivalis in serum from patients with a history of destructive periodontal disease

In vitro environmental regulation of Porphyromonas gingivalis growth and virulence

In vivo induction of proinflammatory cytokines in mouse tissue by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans

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