Mechanisms for osteogenic differentiation of human mesenchymal stem cells induced by fluid shear stress

Liu, Liyue; Yuan, Wenji; Wang, Jinfu

doi:10.1007/s10237-010-0206-x

Cited by 86 publications

(67 citation statements)

References 122 publications

(159 reference statements)

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“…Shear stress-induced proliferation of osteocytes, MSCs, as well as chondrocytes has been reported [32][33][34], involving several mechanisms such as the ERK pathway, MAP kinase pathway, NO/cGMP/PKG, and calcium signaling [35]. In addition, mechanical signaling through the cytoskeleton linkage between focal adhesion and regulators of cellular contractility contribute to the regulation of cell proliferation [36].…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein

Zhong

Tian

Zheng

et al. 2013

Stem Cells and Development

105

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Mechanical cues exert considerable influence on the fates of stem cells and terminally differentiated chondrocytes. The elucidation of the interactions between cell fate and mechanical cues in nuclear mechanotransduction will provide new clues to modulate tissue homeostasis and regeneration. In this study, we used an integrated microfluidic perfusion device to simultaneously generate multiple-parameter fluid shear stresses to investigate the role of fluid flow stimuli in the regulation of Yes-associated protein (YAP) expression and the fates of mesenchymal stem cells (MSCs) and primary chondrocytes. YAP expression was regulated by the level of fluid flow stimulus in both MSCs and chondrocytes. An increase in the magnitude of stimulation enhanced the expression of YAP, ultimately resulting in an increase in osteogenesis and a decrease in adipogenesis for MSCs, and initiating dedifferentiation for chondrocytes. Cytochalasin D not only repressed nuclear YAP accumulation in the flow state, but also abrogated flow-induced effects on MSC differentiation and the chondrocyte phenotype, resulting in MSC adipogenesis and the maintenance of the chondrocyte phenotype. Our findings reveal the connection between YAP and MSC/chondrocyte fates in a fluid flow-induced mechanical microenvironment and provide new insights into the mechanisms by which mechanical cues regulate the fates of MSCs and chondrocytes.

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Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein

Zhong

Tian

Zheng

et al. 2013

Stem Cells and Development

105

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“…The culture microenvironment is an important factor that influences the differentiation direction of hMSCs. Mechanical stimuli, such as compression and fluid shear stress (FSS), are important culture microenvironment factors that can regulate the differentiation of hMSCs (Liu et al 2010;Simmons et al 2003;Haudenschild et al 2009). FSS is a shear stress incurred by fluid moving along the solid boundary and loaded on this boundary.…”

Section: Introductionmentioning

confidence: 99%

Different effects of intermittent and continuous fluid shear stresses on osteogenic differentiation of human mesenchymal stem cells

Liu

Chen

et al. 2011

Biomech Model Mechanobiol

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A reasonable mechanical microenvironment similar to the bone microenvironment in vivo is critical to the formation of engineering bone tissues. As fluid shear stress (FSS) produced by perfusion culture system can lead to the osteogenic differentiation of human mesenchymal stem cells (hMSCs), it is widely used in studies of bone tissue engineering. However, effects of FSS on the differentiation of hMSCs largely depend on the FSS application manner. It is interesting how different FSS application manners influence the differentiation of hMSCs. In this study, we examined the effects of intermittent FSS and continuous FSS on the osteogenic differentiation of hMSCs. The phosphorylation level of ERK1/2 and FAK is measured to investigate the effects of different FSS application manners on the activation of signaling molecules. The results showed that intermittent FSS could promote the osteogenic differentiation of hMSCs. The expression level of osteogenic genes and the alkaline phosphatase (ALP) activity in cells under intermittent FSS application were significantly higher than those in cells under continuous FSS application. Moreover, intermittent FSS up-regulated the activity of ERK1/2 and FAK. Our study demonstrated that intermittent FSS is more effective to induce the osteogenic differentiation of hMSCs than continuous FSS.

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“…4. An alternate proposed pathway also shown here was highlighted in a review by Liu et al (2010). Integrinα5β1, upregulated in ASCs with applied fluid shear stress, has been separately identified as important in promoting osteogenesis through ERK 1/2 activation .…”

Section: Indirect and Chemical Mechanisms: Receptors Ion Channels Gmentioning

confidence: 52%

Review of biophysical factors affecting osteogenic differentiation of human adult adipose-derived stem cells

Salazar

Onodera

2012

Biophys Rev

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Developing bone is subject to the control of a broad variety of influences in vivo. For bone repair applications, in vitro osteogenic assays are routinely used to test the responses of bone-forming cells to drugs, hormones, and biomaterials. Results of these assays are used to predict the behavior of bone-forming cells in vivo. Stem cell research has shown promise for enhancing bone repair. In vitro osteogenic assays to test the bone-forming response of stem cells typically use chemical solutions. Stem cell in vitro osteogenic assays often neglect important biophysical cues, such as the forces associated with regular weight-bearing exercise, which promote bone formation. Incorporating more biophysical cues that promote bone formation would improve in vitro osteogenic assays for stem cells. Improved in vitro osteogenic stimulation opens opportunities for "preconditioning" cells to differentiate towards the desired lineage. In this review, we explore the role of select biophysical factors-growth surfaces, tensile strain, fluid flow and electromagnetic stimulation-in promoting osteogenic differentiation of stem cells from human adipose. Emphasis is placed on the potential for physical microenvironment manipulation to translate tissue engineering and stem cell research into widespread clinical usage.

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Mechanisms for osteogenic differentiation of human mesenchymal stem cells induced by fluid shear stress

Cited by 86 publications

References 122 publications

Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein

Mesenchymal Stem Cell and Chondrocyte Fates in a Multishear Microdevice Are Regulated by Yes-Associated Protein

Different effects of intermittent and continuous fluid shear stresses on osteogenic differentiation of human mesenchymal stem cells

Review of biophysical factors affecting osteogenic differentiation of human adult adipose-derived stem cells

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