The aim of the present research was to examine the process of bioleaching and the application of a combined process for the recovery of copper and nickel from industrial sand deposits. The investigated sample of sands finer than 0.1 mm in size contained 0.32% Ni and 0.22% Cu. Industrial sands were processed by bioleaching in flasks on a thermostatically controlled shaker. In addition, sand roasting experiments were carried out with ammonium sulfate. An attempt was also made to use a combined process, including low-temperature roasting of the sands mixed with ammonium sulfate, water-leaching of the roasted mixture, and subsequent biological after-leaching of the residue. In the process of roasting the industrial sands in a mixture including ammonium sulfate at a temperature of 400 °C, more than 70% of the non-ferrous metals were recovered. We examined the possibility of recovering non-ferrous metals using a combined process including low-temperature roasting of industrial sands and the additional recovery of non-ferrous metals by bioleaching using the Acidithiobacillus ferrivorans bacterial strain, which was found to increase the recovery of non-ferrous metals to up to 90%.