2000
DOI: 10.1006/bbrc.2000.3723
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Mechanisms for the Formation of Protein-Bound Homocysteine in Human Plasma

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Cited by 38 publications
(35 citation statements)
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“…Binding of Hcys to plasma proteins is biphasic. First reaction, involving displacement of cysteine from plasma proteins, is rapid and oxygen independent, while the second reaction constitutes slower, oxygen-dependent thiol oxidation (Togawa et al 2000). Our present results showed that not only the reduced form of Hcys, but also its cyclic thioester may diminish the amount of free protein thiol concentration, especially when blood plasma is exposed for a long incubation time (60 min) (Fig.…”
supporting
confidence: 48%
“…Binding of Hcys to plasma proteins is biphasic. First reaction, involving displacement of cysteine from plasma proteins, is rapid and oxygen independent, while the second reaction constitutes slower, oxygen-dependent thiol oxidation (Togawa et al 2000). Our present results showed that not only the reduced form of Hcys, but also its cyclic thioester may diminish the amount of free protein thiol concentration, especially when blood plasma is exposed for a long incubation time (60 min) (Fig.…”
supporting
confidence: 48%
“…The filtrate was then applied to an analytical Vydac C-4 HPLC column (25 ϫ 0.46 cm, 5-m particle size) and eluted at 0.75 ml/min over 30 min using a gradient of 40 -85% acetonitrile. Transthyretin and its Cys 10 conjugates eluted between 52 and 54% acetonitrile. The solvent mixture was removed from the protein using a Speedvac concentrator.…”
Section: Isolation and Purification Of Transthyretin Frommentioning
confidence: 99%
“…Plasma-We recently determined the equilibrium binding capacity of plasma proteins for homocysteine but did not identify the specific proteins responsible for the binding (14). To identify specific homocysteine-binding proteins, human plasma was incubated with 35 S-L-homocysteine.…”
Section: Identification Of Albumin As a Binding Protein For Homocystementioning
confidence: 99%
“…However, in patients with homocystinuria, tHcy levels approach 0.5 mM (13). The overall in vitro binding capacity of human plasma proteins for homocysteine is Ͼ0.4 mM (14). Almost all pathophysiology studies utilize free reduced homocysteine (reviewed in Ref.…”
mentioning
confidence: 99%