Mechanisms of antibiotic resistance in Pseudomonas aeruginosa biofilms

Fernández-Billón, María; Llambías-Cabot, Aina E.; Jordana‐Lluch, Elena; Oliver, Antonio; Macià, María D.

doi:10.1016/j.bioflm.2023.100129

Cited by 30 publications

(8 citation statements)

References 135 publications

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“…In chronic P. aeruginosa infections, the typical biofilm lifestyle significantly diminishes the effectiveness of antimicrobial treatments, as it results in inherent tolerance. This tolerance encompasses physical and physiological factors, along with biofilm-specific genes that can temporarily shield against antibiotics, thereby fostering the emergence of resistance [80]. The negatively charged polysaccharides (especially the Pel polysaccharide and alginate of P. aeruginosa) can effectively sequestrate the positively charged aminoglycoside class of antibiotics, such as tobramycin, thus preventing them from penetrating the deeper layers of the biofilm [81].…”

Section: Bacterial Biofilm Resistancementioning

confidence: 99%

Synergistic Effect of Plant Compounds in Combination with Conventional Antimicrobials against Biofilm of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida spp.

Bonincontro,

Scuderi,

Marino

et al. 2023

Pharmaceuticals

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Bacterial and fungal biofilm has increased antibiotic resistance and plays an essential role in many persistent diseases. Biofilm-associated chronic infections are difficult to treat and reduce the efficacy of medical devices. This global problem has prompted extensive research to find alternative strategies to fight microbial chronic infections. Plant bioactive metabolites with antibiofilm activity are known to be potential resources to alleviate this problem. The phytochemical screening of some medicinal plants showed different active groups, such as stilbenes, tannins, alkaloids, terpenes, polyphenolics, flavonoids, lignans, quinones, and coumarins. Synergistic effects can be observed in the interaction between plant compounds and conventional drugs. This review analyses and summarises the current knowledge on the synergistic effects of plant metabolites in combination with conventional antimicrobials against biofilms of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. The synergism of conventional antimicrobials with plant compounds can modify and inhibit the mechanisms of acquired resistance, reduce undesirable effects, and obtain an appropriate therapeutic effect at lower doses. A deeper knowledge of these combinations and of their possible antibiofilm targets is needed to develop next-generation novel antimicrobials and/or improve current antimicrobials to fight drug-resistant infections attributed to biofilm.

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Section: Bacterial Biofilm Resistancementioning

confidence: 99%

Synergistic Effect of Plant Compounds in Combination with Conventional Antimicrobials against Biofilm of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida spp.

Bonincontro,

Scuderi,

Marino

et al. 2023

Pharmaceuticals

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show abstract

“…Due to difficult eradication, these antimicrobial-resistant communities of P. aeruginosa can cause a fatal decline in CF lung function [ 27 , 94 ]. Despite expanding knowledge regarding P. aeruginosa biofilm, developing new alternative therapeutic approaches in clinical practice is required, for instance, nanoparticles, quorum sensing inhibitors (QSI), anti-biofilm compounds, and CRISPR gene editing [ 140 ].…”

Section: Pathogenesis Of P Aeruginosa In Cfmentioning

confidence: 99%

Bile effects on the Pseudomonas aeruginosa pathogenesis in cystic fibrosis patients with gastroesophageal reflux

Bayat,

Nahand,

Farsad-Akhatr

et al. 2023

Heliyon

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“…In addition, the capability of EPS to limit the penetration of anti-microbial agents allows the minimum inhibitory concentration (MIC) of anti-microbial agents on P. aeruginosa biofilms to be 100-1000 times higher than that required to treat bacterioplankton [17,18]. Biofilms are one of the main factors responsible for the development of resistance to antibiotics in P. aeruginosa [19,20]. Currently, various bacteriostatic agents such as anti-microbial peptides and phages, as well as the related mechanisms, have been extensively studied.…”

Section: Introductionmentioning

confidence: 99%

The Anti-Microbial Peptide Citrocin Controls Pseudomonas aeruginosa Biofilms by Breaking Down Extracellular Polysaccharide

Wang,

Zheng,

Hou

et al. 2024

IJMS

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Citrocin is an anti-microbial peptide that holds great potential in animal feed. This study evaluates the anti-microbial and anti-biofilm properties of Citrocin and explores the mechanism of action of Citrocin on the biofilm of P. aeruginosa. The results showed that Citrocin had a significant inhibitory effect on the growth of P. aeruginosa with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 0.3 mg/mL. All five concentrations (1/4MIC, 1/2MIC, MIC, 2MIC, and 4MIC) of Citrocin inhibited P. aeruginosa biofilm formation. Citrocin at the MIC, 2MIC and 4MIC removed 42.7%, 76.0% and 83.2% of mature biofilms, respectively, and suppressed the swarming motility, biofilm metabolic activity and extracellular polysaccharide production of P. aeruginosa. Metabolomics analysis indicated that 0.3 mg/mL of Citrocin up- regulated 26 and down-regulated 83 metabolites, mainly comprising amino acids, fatty acids, organic acids and sugars. Glucose and amino acid metabolic pathways, including starch and sucrose metabolism as well as arginine and proline metabolism, were highly enriched by Citrocin. In summary, our research reveals the anti-biofilm mechanism of Citrocin at the metabolic level, which provides theoretical support for the development of novel anti-biofilm strategies for combatting P. aeruginosa.

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Mechanisms of antibiotic resistance in Pseudomonas aeruginosa biofilms

Cited by 30 publications

References 135 publications

Synergistic Effect of Plant Compounds in Combination with Conventional Antimicrobials against Biofilm of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida spp.

Synergistic Effect of Plant Compounds in Combination with Conventional Antimicrobials against Biofilm of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida spp.

Bile effects on the Pseudomonas aeruginosa pathogenesis in cystic fibrosis patients with gastroesophageal reflux

The Anti-Microbial Peptide Citrocin Controls Pseudomonas aeruginosa Biofilms by Breaking Down Extracellular Polysaccharide

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