Mechanisms of association of Candida albicans with intestinal mucosa

Cecal Colonization and Systemic Spread of Candida albicans in Mice Treated with Antibiotics and Dexamethasone

“…Although C. albicans can colonize all portions of the gastrointestinal tract, colonization is typically maximal in the cecum of the adult mouse, and the cecum is most often used to monitor candida colonization in mice (32,(35)(36)(37)(38)(39) (40) without uracil for cultivation of yeast containing the URA3 gene. The lower limit of assay detection was 3.0 log 10 /g of cecum.…”

“…Before excision of the cecum, the MLN and kidneys were aseptically excised, homogenized, and quantitatively cultured as described (33,39). Tissue homogenates were plated on tryptic soy agar supplemented with 5% sheep red blood cells and minimal medium agar without uracil.…”

Gastrointestinal Colonization by Candida albicans Mutant Strains in Antibiotic-Treated Mice

“…The concentration of intestinal C. albicans has often been correlated with the incidence of systemic infection, and several investigators (including ourselves) have assumed that the cecum is a representative site that can be used to monitor mouse GI colonization by C. albicans (2,6,(12)(13)(14)(15)(16), although the reasons for this choice have largely been anecdotal.…”

Experimental Model of Candida Albicans (Serotypes a and B) Adherence in Vitro

“…There is evidence that C. albicans may produce more than one adhering structure or that more than one of these structures, such as proteins, chitin and lipids, may be involved in this process. Most reports in the literature indicate a mannoprotein as the adhesin (9,18,30,33). The receptors of various tissues to which C. albicans binds have not been well characterized, although several investigators have suggested that they may be fibronectins, certain phospholipids, L-fucose, mannose, Nacetyl-D-glucosamine, mucins, laminins, and collagen (9,12,20,40).…”

“…The receptors of various tissues to which C. albicans binds have not been well characterized, although several investigators have suggested that they may be fibronectins, certain phospholipids, L-fucose, mannose, Nacetyl-D-glucosamine, mucins, laminins, and collagen (9,12,20,40). Because of the importance and the significance of adherence, several in vivo and in vitro models have been developed to quantitate and characterize C. albicans adherence to cellular and inanimate surfaces (4,6,7,9,10,11,13,16,17,18,19,20,22,23,24,26,34,35). These studies have shown that various factors interfere with yeast adherence to epithelial cells Departmento de Microbiologia, Instituto de Ciências Biomédicas -Universidade de São Paulo.…”