Mechanisms of Avidity Modulation in Leukocyte Adhesion Studied by AFM

Wojcikiewicz, Ewa P.; Moy, Vincent T.

doi:10.1002/0470007702.ch10

Cited by 1 publication

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References 25 publications

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“…While these systems are more heterogenous and the results harder to interpret, they maintain the complexity and integrity of a native cellular system, and also allow for probing ligand binding to both intra and extracellular membrane tethered multivalent species. Finally, atomic force microscopy (AFM) has also been utilized to obtain avidities of antibody-antigen interactions or cell-cell adhesion strength (Wojcikiewicz and Moy, 2005 ; Zhang et al, 2020 ).…”

Section: Measuring and Modeling Aviditymentioning

confidence: 99%

Binding Revisited—Avidity in Cellular Function and Signaling

Erlendsson

Teilum

2021

Front. Mol. Biosci.

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When characterizing biomolecular interactions, avidity, is an umbrella term used to describe the accumulated strength of multiple specific and unspecific interactions between two or more interaction partners. In contrast to the affinity, which is often sufficient to describe monovalent interactions in solution and where the binding strength can be accurately determined by considering only the relationship between the microscopic association and dissociation rates, the avidity is a phenomenological macroscopic parameter linked to several microscopic events. Avidity also covers potential effects of reduced dimensionality and/or hindered diffusion observed at or near surfaces e.g., at the cell membrane. Avidity is often used to describe the discrepancy or the “extra on top” when cellular interactions display binding that are several orders of magnitude stronger than those estimated in vitro. Here we review the principles and theoretical frameworks governing avidity in biological systems and the methods for predicting and simulating avidity. While the avidity and effects thereof are well-understood for extracellular biomolecular interactions, we present here examples of, and discuss how, avidity and the underlying kinetics influences intracellular signaling processes.

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Section: Measuring and Modeling Aviditymentioning

confidence: 99%