Mechanisms of biogenic amine transport and storage IV. Relationship between K+ and the Na+ requirement for transport and storage of 5-hydroxytryptamine and norepinephrine in synaptosomes

Bogdanski, Donald F.; Blaszkowski, Thomas P.; Tissari, Anja H.

doi:10.1016/0005-2736(70)90257-9

Cited by 76 publications

(14 citation statements)

References 25 publications

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“…Excess K+ partially reverses the effects of ouabain, suggesting that ouabain causes a functional de ficiency of the K+ used for the energy producing aspect of the transport process. The data are in accord with our view that a Na+ gradient alone can provide energy for transport [4,8,9], although in certain conditions simultaneous electrolyte transport is required to maintain amine trans port [6],…”

Section: Introductionsupporting

confidence: 80%

“…Earlier reports had indicated that ouabain begins to block the accumu lation of 5-HT in synaptosomes after a lapse of time [32] and that K f -free media also block the accumulation and transport of 5-HT after a similar lapse of time [6]. In accord with previous data, ouabain inhibited the trans port of 5-HT 62% at 5 min and 80% at 20 min, corresponding to per centage inhibitions of 50 and 81% by K+-free media.…”

Section: Delayed Action Of K+-lack and Ouabain On The Rates Of Accumumentioning

confidence: 99%

“…More recently, we have suggested that the transport of amines may be dependent upon the simultaneous transport of electrolytes maintaining local concentration gradients of these electrolytes at the plasma membrane [6].…”

Section: Introductionmentioning

confidence: 99%

“…Low concentrations of K+ facilitate the transport and retention of NE [3,4,6] or metaraminol by sympathetic nerve endings in heart slices [28] and of serotonin (5-HT) and NE by synaptosomes [7,8,13,32]. Like ouabain, potassium-free media also inhibit (Na+-f K+)-ATPase [32], the enzyme that provides for the energy requirement for transport.…”

Section: Introductionmentioning

confidence: 99%

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Biogenic Amine Transport

Tissari¹,

Bogdanski²

1971

Pharmacology

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Comparisons were made of the inhibitory effects of ouabain and potassium-deficient media on the transport of sero tonin and norepinephrine by synaptosomes. The following parallel effects were observed in both instances. Substrate introduced into the media was transported for up to 5 min, then stopped. Pre liminary incubation of synaptosomes in the above media, before substrate was introduced into the beaker, prevented the initial transport. Inhibition of transport was dependent upon the con centration of external sodium, being greater with 143 mM than with 50 mM sodium. The inhibitory effect of ouabain on transport was antagonized by high concentrations of potassium. It is concluded that ouabain blocks transport by blocking a potassium-requiring factor in transport, possibly (Na⁺ + K⁺)-ATPase. Thus, potassium deficiency and ouabain inhibit transport indirectly by inhibiting the energy-producing aspect of transport. The action of ouabain may include a direct component, especially in the case of norepinephrine.

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Section: Introductionsupporting

confidence: 80%

Section: Delayed Action Of K+-lack and Ouabain On The Rates Of Accumumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biogenic Amine Transport

Tissari¹,

Bogdanski²

1971

Pharmacology

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“…A possible interaction between potassium and tissue neural elements is suggested by the fact that the accumulation of various tissue amines is altered in a complex fashion by potassium ion (41)(42)(43). As an indication that this effect may be a real factor in the microvascular response to potassium, Sudak and Fulton (44) found that the cheek pouch vasculature constricts when the plasma potassium concentration is increased.…”

Section: Dulingmentioning

confidence: 99%

Effects of potassium ion on the microcirculation of the hamster.

Duling¹

1975

Circulation Research

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The vasoactive properties of potassium were assessed in the microcirculation of the hamster cremaster muscle and the muscular and epithelial portions of the hamster cheek pouch. Tissues were transilluminated and suffused with a physiological salt solution whose potassium concentration varied from 0 to 20 mM. Vessel diameters were measured and normalized as a percent of the control diameter (± SE) observed during exposure to 4.7 mM K + . Altering the potassium concentration in the suffusion solution caused a transient vascular response. The peak changes in the vascular diameter of the arterioles supplying striated muscle varied directly with the suffusion solution potassium concentration from a minimum of 78 ± 3% in 0 mM K + to 155 ± 15% in 15 mM K". Vascular diameter increases were sustained for the full 5-minute test period only in 15 mM K+. In the epithelial portions of the cheek pouch, only the constrictor component of the potassium response was observed. The data indicate that potassium is sufficiently potent to participate in initiating functional hyperemia in striated muscle and might cause as much as a 6.3-fold increase in flow. Functional hyperemias exceeding approximately 3 minutes cannot be due to potassium ion, since the dilation induced by this agent is transient.• Cells contain a large store of labile potassium that can be released by various stimuli such as muscle contraction and hypoxia (1-9). Many investigators have shown that potassium at the increased concentrations observed in venous blood during exercise and hypoxia can relax smooth muscle both in vivo (5)(6)(7)(8)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) and in vitro (21-31). Therefore, this ion. may be important in the local control of blood flow. However, further quantification of the reactivity of resistance vessels to the ion must be carried out before accurate estimates of the importance of potassium in blood flow regulation can be made. These data can be obtained by studying microvessel responses to alterations in suffusion solution potassium concentrations, but such experiments have only been performed on pial vessels (11,16,17). Furthermore, no data are currently available showing the temporal response pattern of microvessels during variations in potassium concentration. These data are of particular interest in view of the transient potassium ion release during functional hyperemia in striated muscle (7,8) and the transient vascular smooth muscle response to potassium both in vitro (26-32) and in vivo (10). A preliminary report of this work was presented at the Fall Meeting of the American Physiological Society, August, 1974. This work was done during Dr. Duling's tenure as an Established Investigator of the American Heart Association.Received September 20, 1974. Accepted for publication June 4, 1975. I have therefore studied the responses of the arterioles of the hamster cremaster muscle and cheek pouch to changes in the potassium concentration of a superfusion solution. The aims of the experiment were to: (1) estab...

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Local Control of Vascular Resistance

Haddy¹

1974

Arch Intern Med

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Mechanisms of biogenic amine transport and storage IV. Relationship between K+ and the Na+ requirement for transport and storage of 5-hydroxytryptamine and norepinephrine in synaptosomes

Cited by 76 publications

References 25 publications

Biogenic Amine Transport

Biogenic Amine Transport

Effects of potassium ion on the microcirculation of the hamster.

Local Control of Vascular Resistance

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