Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats

Ćelić, Tanja; Omrčen, Hrvoje; Španjol, Josip; Bobinac, Dragica

doi:10.1016/j.transproceed.2018.08.035

Cited by 5 publications

(1 citation statement)

References 34 publications

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“…The extensive glomerulosclerosis in the remnant kidney model may be closely related to immune dysregulation and abnormal activation of the renin-angiotensin system [18]. In addition, the growth factor BMP-7 plays an irreplaceable role in the repair and regeneration of kidney tissue [19]. The results showed that BMP-7 expression in renal tissue was significantly increased 12 weeks after CRF was treated with MSC-loaded GMs.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cells Loaded with Gelatin Microcryogels Attenuate Renal Fibrosis

Geng

Hong

Chi

et al. 2019

BioMed Research International

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Background The treatment of chronic kidney diseases (CKDs) by different approaches using mesenchymal stem cells (MSCs) has made great strides. In this study, we aimed to explore the potential mechanism of gelatin microcryogels (GMs) as a cell therapeutic vector to block the progression of CKD. Methods In vivo, the pedicled omentum valve with MSC-loaded GMs was packed onto 5/6 nephrectomized kidneys derived from rats. The therapeutic effects were evaluated. In vitro, TNF-α, TGF-β, and MSCs were added to the medium of the HK-2 cell culture system, and key genes involved in anti-inflammatory and antifibrosis effects were evaluated by qPCR. Results After 12 weeks of MSC transplantation, kidney functions, such as serum creatinine, urea nitrogen, and 24-hour urine protein, were significantly improved. The pedicled omentum valve was packed with MSC-loaded GMs onto the 5/6 nephrectomized kidney, and the expressions of collagen IV, α-SMA, and TGF-β were all evaluated by immunohistochemical staining and western blot analysis. MSC-loaded-GMs also showed antifibrotic effects by inducing the upregulation of HO-1, BMP-7, and HGF and the downregulation of MCP-1 at the mRNA level. Four weeks after MSC-loaded GM treatment, we found that the mRNA levels of TNF-α and IL-6 were clearly reduced. MSC-conditional medium (MSC-CM) showed that the TNF-α-induced expression of IL-8 and IL-6 mRNA was reversed; E-cadherin mRNA was upregulated; and the TGF-β-induced expression of collagen IV, α-SMA, and fibronectin (FN) mRNA in HK-2 cells was reduced. Conclusions We demonstrated that the pedicled omentum valve packed with MSC-loaded GMs had a renal protective effect on the 5/6 nephrectomized kidney by observing the anti-inflammatory and antifibrosis effects.

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Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cells Loaded with Gelatin Microcryogels Attenuate Renal Fibrosis

Geng

Hong

Chi

et al. 2019

BioMed Research International

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Zaprinast and avanafil increase the vascular endothelial growth factor, vitamin D₃, bone morphogenic proteins 4 and 7 levels in the kidney tissue of male rats applied the glucocorticoid

Huyut

Bakan

Akbay

et al. 2020

Archives of Physiology and Biochemistry

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Expression of sclerostin and bone morphogenetic protein-7 (BMP-7) in serum of patients with chronic kidney disease-mineral and bone disorder (CKD-MBD) and their correlation with calcium and phosphorus metabolism

TAO

et al. 2023

Food Sci. Technol

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Calcium and phosphorus metabolism involves in patients with chronic kidney disease-mineral and bone disorder (CKD-MBD). Sclerostin and bone morphogenetic protein-7 (BMP-7) are closely related to bone formation. This study aims to assess sclerostin and BMP-7's role in renal function, blood calcium and phosphorus in CKD-MBD. 86 CKD-MBD patients were collected as the case group and 86 healthy subjects were selected as control group. ELISA was used to detect Sclerostin and BMP-7 serum level. The automatic biochemical analyzer was used to detect liver and kidney function. Blood calcium and phosphorus level, and iPHT level was detected by immunoradiation. No differences were found regarding age, gender, and BMI between case group and control group (P > 0.05). Compared to controls, CKD-MBD patients had significantly reduced blood calcium and eGFR level, and increased Scr, BUN, blood phosphorus and iPTH level (P < 0.05) with increased Sclerostin (t = 33.86, P < 0.001), and reduced BMP-7 level (t = 329.38, P < 0.001). Scr, BUN, blood phosphorus levels and iPTH were correlated with Sclerostin positively (P < 0.05) and BMP-7 negatively (P < 0.05). eGFR and blood calcium were correlated with Sclerostin negatively (P < 0.05) and BMP-7 positively (P < 0.05). Scr, eGFR, blood calcium levels, blood phosphorus levels, and iPTH are independent risk factors for serum Sclerostin and BMP-7 level. Sclerostin and BMP-7 changes may be important factors affecting the occurrence of CKD-MBD. Renal function, blood calcium and blood phosphorus are independent risk factors for serum Sclerostin and BMP-7 level.

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Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats

Cited by 5 publications

References 34 publications

Mesenchymal Stem Cells Loaded with Gelatin Microcryogels Attenuate Renal Fibrosis

Mesenchymal Stem Cells Loaded with Gelatin Microcryogels Attenuate Renal Fibrosis

Zaprinast and avanafil increase the vascular endothelial growth factor, vitamin D₃, bone morphogenic proteins 4 and 7 levels in the kidney tissue of male rats applied the glucocorticoid

Expression of sclerostin and bone morphogenetic protein-7 (BMP-7) in serum of patients with chronic kidney disease-mineral and bone disorder (CKD-MBD) and their correlation with calcium and phosphorus metabolism

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Mechanisms of Bone Morphogenetic Protein-7 Protective Effects Against Cold Ischemia-Induced Renal Injury in Rats

Cited by 5 publications

References 34 publications

Mesenchymal Stem Cells Loaded with Gelatin Microcryogels Attenuate Renal Fibrosis

Mesenchymal Stem Cells Loaded with Gelatin Microcryogels Attenuate Renal Fibrosis

Zaprinast and avanafil increase the vascular endothelial growth factor, vitamin D3, bone morphogenic proteins 4 and 7 levels in the kidney tissue of male rats applied the glucocorticoid

Expression of sclerostin and bone morphogenetic protein-7 (BMP-7) in serum of patients with chronic kidney disease-mineral and bone disorder (CKD-MBD) and their correlation with calcium and phosphorus metabolism

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Zaprinast and avanafil increase the vascular endothelial growth factor, vitamin D₃, bone morphogenic proteins 4 and 7 levels in the kidney tissue of male rats applied the glucocorticoid