Mechanisms of brain renin angiotensin system-induced drinking and blood pressure: importance of the subfornical organ

Coble, Jeffrey; Grobe, Justin L; Johnson, Alan Kim; Sigmund, Curt D.

doi:10.1152/ajpregu.00486.2014

Cited by 84 publications

(78 citation statements)

References 151 publications

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“…THE CENTRAL NERVOUS SYSTEM plays a pivotal role in body fluid homeostasis through the ability of specialized neurons in the forebrain lamina terminalis to detect and integrate neurohumoral signals and subsequently alter behavior, neuroendocrine function, and autonomic outflow (Coble et al 2015;McKinley et al 2004;Toney and Stocker 2010). The lamina terminalis spans the rostral wall of the 3rd ventricle and includes two circumventricular organs: the subfornical organ (SFO) and organum vasculosum of the lamina terminalis (OVLT).…”

Section: Subfornical Organ (Sfo) Neurons Play a Pivotal Role In Body mentioning

confidence: 99%

DREADD-induced activation of subfornical organ neurons stimulates thirst and salt appetite

Nation

Nicoleau

Kinsman

et al. 2016

Journal of Neurophysiology

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Nation HL, Nicoleau M, Kinsman BJ, Browning KN, Stocker SD. DREADD-induced activation of subfornical organ neurons stimulates thirst and salt appetite. J Neurophysiol 115: 3123-3129, 2016. First published March 30, 2016 doi:10.1152/jn.00149.2016.-The subfornical organ (SFO) plays a pivotal role in body fluid homeostasis through its ability to integrate neurohumoral signals and subsequently alter behavior, neuroendocrine function, and autonomic outflow. The purpose of the present study was to evaluate whether selective activation of SFO neurons using virally mediated expression of Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) stimulated thirst and salt appetite. Male C57BL/6 mice (12-15 wk) received an injection of rAAV2-CaMKII-HAhM3D(Gq)-IRES-mCitrine targeted at the SFO. Two weeks later, acute injection of clozapine N-oxide (CNO) produced dose-dependent increases in water intake of mice with DREADD expression in the SFO. CNO also stimulated the ingestion of 0.3 M NaCl. Acute injection of CNO significantly increased the number of Fos-positive nuclei in the SFO of mice with robust DREADD expression. Furthermore, in vivo single-unit recordings demonstrate that CNO significantly increases the discharge frequency of both ANG II-and NaCl-responsive neurons. In vitro current-clamp recordings confirm that bath application of CNO produces a significant membrane depolarization and increase in action potential frequency. In a final set of experiments, chronic administration of CNO approximately doubled 24-h water intake without an effect on salt appetite. These findings demonstrate that DREADD-induced activation of SFO neurons stimulates thirst and that DREADDs are a useful tool to acutely or chronically manipulate neuronal circuits influencing body fluid homeostasis.

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Section: Subfornical Organ (Sfo) Neurons Play a Pivotal Role In Body mentioning

confidence: 99%

DREADD-induced activation of subfornical organ neurons stimulates thirst and salt appetite

Nation

Nicoleau

Kinsman

et al. 2016

Journal of Neurophysiology

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“…В последние десятилетия все больше внимания уделя-ется РАС мозга и ее участию в регуляции симпатической активности и артериального давления (Coble et al, 2015). Выполненное нами исследование активности генов дан-ной системы в отделах мозга -гипоталамусе и стволе мозга -выявило наличие существенных межлинейных различий: экспрессия ренинового гена была повышена в гипоталамусе крыс НИСАГ, а в стволе мозга отмечено достоверное увеличение концентрации мРНК гена Асе.…”

Section: моделирование патологий вавиловский журнал генетики и селекцunclassified

“…Однако литера-турные данные о проницаемости гематоэнцефалического барьера для некоторых пептидов, в частности для ангио-тензина II, дают определенную надежду на объяснение повышенного содержания «тандема» ангиотензин -аль-достерон в крови крыс НИСАГ выходом ангиотензина II из некоторых отделов мозга. Известно, что в области циркумвентрикулярных органов, к которым относятся субфорникальный орган, сосудистый орган терминальной пластинки и area postrema (самое заднее поле гипотала-муса), гематоэнцефалический барьер является наиболее проницаемым, что позволяет таким низкомолекулярным пептидам крови, как ангиотензин II, взаимодействовать с данными областями мозга (Coble et al, 2015). При гипертонии показано нарушение целостности гематоэн-цефалического барьера (Pelisch et al, 2011;Biancardi et al, 2014;Biancardi, Stern, 2016), благодаря чему ангиотен-зин II из циркулирующей крови может также проникать в паравентрикулярное ядро гипоталамуса и ростральный отдел вентролатерального продолговатого мозга, вызывая повышение активности симпатической нервной системы.…”

Section: моделирование патологий вавиловский журнал генетики и селекцunclassified

Renin-angiotensin-aldosterone system in ISIAH rats with stressinduced arterial hypertension

Dubinina¹,

Antonov²,

Fedoseeva³

et al. 2016

Vestn. VOGiS

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Because the renin-angiotensin system (RAS) has a wide range of opportunities in the regulation of fluid and electrolyte balance and arterial pressure, it is currently hypothesized that alterations in systemic circulating or local tissue RAS are some of the most important pathogenetic factors in the development of essential hypertension. The aim of the study was to investigate circulating and local tissue RAS activities in ISIAH rats with stress-induced arterial hypertension. We estimated the serum levels of renin, the angiotensin-convert ing enzyme, angiotensin II and aldosterone by an enzymelinked immunosorbent assay, and mRNA expression of RAS genes in kidney, adrenals and brain tissues was measured by the real-time polymerase chain reaction. The mRNA expression of the renin gene (Ren) in the ISIAH rats was significantly decreased as compared to the normotensive WAG rats, but plasma renin concentrations had no difference. At the same time, the serum levels of angiotensin II and aldosterone in the ISIAH rats were enhanced, which suggests the existence of an ectopic site of angiotensin synthesis. Expression of RAS genes in the adrenals of hypertensive rats was unchanged. By contrast, a significant increase of RAS genes expression was found in the brain tissues. The mRNA of the Ren gene was increased in the hypothalamus, and the mRNA of Ace gene was increased in the brain stem of the ISIAH rats. This may be indicative of a local increase of RAS activity in the brain tissues of ISIAH rats. Nevertheless, the results of the study define ISIAH rat strain as a model of human low-renin hypertension.Key words: ISIAH rat strain; renin-angiotensinaldosterone system; low-renin hypertension.Благодаря широкому спектру возможностей ренин-ангиотензи-новой системы (РАС) в регуляции водно-солевого баланса и арте-ри ального давления изменение ее активности на системном или локальном (тканевом) уровнях в настоящее время рассматривает-ся как один из наиболее значимых патогенетических факторов развития эссенциальной гипертонии. Цель работы -исследование циркуляторной и тканевой РАС у крыс НИСАГ со стресс-индуциро-ванной артериальной гипертензией. Была изме рена концентрация ренина, ангиотензин-превращающего фер мента, ангиотензина II и альдостерона в сыворотке крови методом иммуноферментного анализа, а также исследована экспрессия основных генов РАС в почке, надпочечнике и отделах мозга с помощью полимеразной цепной реакции в реальном времени. Обнаружено, что в почке крыс НИСАГ снижена экспрессия мРНК гена ренина Ren по сравне-нию с нормотензивными крысами WAG, при этом концентрация ренина в сыворотке крови крыс данных линий не отличается. В то же время у крыс этой гипертензив ной линии наблюдалось достоверное увеличение концентраций ангио тензина II и альдо-стерона в сыворотке крови, что может свидетельствовать о нали-чии «эктопического» очага синтеза ангиотензина II. В надпочечни-ках крыс НИСАГ экспрессия генов компонентов РАС не изменена. В структурах мозга показано уве личение экспрессии генов ком-понентов данной системы: Ren -в...

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“…Fluid balance is regulated by neuroendocrine control systems and can be altered in disease states, such as in chronic kidney disease (1)(2)(3) .…”

Section: Introductionmentioning

confidence: 99%

“…Adequate recording of this equation is of great importance for making therapeutic and care decisions (1,6) .…”

Section: Introductionmentioning

confidence: 99%

Water balance in pediatric nephrology: construction of a Standard Operating Procedure

et al. 2018

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Objective: To construct a Standard Operating Procedure (SOP) about the water balance, to be used by the health team in the care of children hospitalized in a pediatric nephrology unit. Method: The study was carried out in two stages: integrative review of the literature for the development of SOP and validation by specialists. The search for literature occurred in the electronic databases PUBMED, SCOPUS, LILACS, BDENF. After the bibliographic survey the construction of the SOP was performed, which was evaluated by specialists. The analysis was performed by calculating the Content Validity Index (CVI). Results: nine studies were selected as results of the integrative review. The sample of specialists was composed of nine professionals. The study was evaluated in six items, five of which presented CVI = 1 and one obtained CVI = 0.77. Conclusion: the evaluation of specialists culminated in the validation of SOP, suggesting changes that were accepted and discussed with the literature.

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Mechanisms of brain renin angiotensin system-induced drinking and blood pressure: importance of the subfornical organ

Cited by 84 publications

References 151 publications

DREADD-induced activation of subfornical organ neurons stimulates thirst and salt appetite

DREADD-induced activation of subfornical organ neurons stimulates thirst and salt appetite

Renin-angiotensin-aldosterone system in ISIAH rats with stressinduced arterial hypertension

Water balance in pediatric nephrology: construction of a Standard Operating Procedure

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