Mechanisms of carbapenem resistance in endemic Pseudomonas aeruginosa isolates after an SPM-1 metallo-β-lactamase producing strain subsided in an intensive care unit of a teaching hospital in Brazil

Cacci, Luciana Camila; Chuster, Stephanie Gomes; Martins, Natacha; Carmo, Pâmella Rodrigues do; Girão, Valéria Brígido de Carvalho; Nouér, Simone Aranha; Freitas, Wania Vasconcelos de; Matos, Juliana Arruda de; Magalhães, Ana Cristina de Gouveia; Ferreira, A.; Picão, Renata Cristina; Moreira, Beatriz Meurer

doi:10.1590/0074-02760160116

Cited by 28 publications

(32 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genes encoding MBLs are transferred by mobile genetic elements [26]. MBL production is noted in massive outbreaks, including the international dissemination of resistant strains [27]. So, early detection of MBL producing P. aeruginosa is very important in terms of determining treatment and preventing the spread of infection with resistant isolates.…”

Section: Discussionmentioning

confidence: 99%

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Müderris

Durmaz

Özdem

et al. 2018

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: In recent years, the prevalence of multidrug-resistant P. aeruginosa has remarkably increased. Thus, we wanted to investigate the carbapenem resistance mechanisms and clonal relationship among 80 carbapenem-resistant P. aeruginosa strains. Methodology: Carbapenemase production was detected using the Modified Hodge Test (MHT), EDTA combined disc method (ECD), and PCR. Expression levels of efflux and porin genes were mesured by real-time reverse transcription PCR. Clonal relationship of the isolates was investigated by pulsed-field gel electrophoresis (PFGE). Results: Carbapenemase production was detected in 7.5% of the isolates with MHT/ECD tests and in 11.3% of the isolates with PCR. Although the specificity of MHT/ECD was high, the sensitivitivity was low. oprD downregulation and mexB, mexY, and mexD overexpression were demonstrated in 55%, 16.3%, 2.5%, and 2.5% of the isolates, respectively. Multiple carbapenem resistance mechanisms were found in nearly a quarter of the isolates. PFGE typing of the 80 P. aeruginosa isolates yielded 61 different patterns. A total of 29 isolates (36.3%) were classified in 10 clusters, containing 2 to 7 strains. We could not find a strict relationship between PFGE profile and carbapenem resistance mechanisms. Conclusions: Although oprD downregulation and MexAB-OprM overexpression were the most common mechanisms, carbapenem resistance was associated with multiple mechanisms in the study. MHT/ECD tests should not be used alone for investigation of carbapenemase production in P. aeruginosa. Rapid tests with high sensitivity and specificity should be developed for the detection of carbapenemase production in P. aeruginosa.

show abstract

Section: Discussionmentioning

confidence: 99%

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Müderris

Durmaz

Özdem

et al. 2018

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…In addition, 373 carbapenemase gene-negative GNB isolates were identi ed. Other mechanisms of carbapenem resistance have been recognized, which include overexpression of Extended Spectrum Beta-Lactamases (ESBLs) or AmpC β-lactamase, decreased membrane permeability due to porin loss, and expression of e ux pumps [17,18]. The complexity of carbapenem resistance mechanisms in GNB and characteristics of the human gastrointestinal tract make the dissemination and transmutation of resistance genes more complex and frequent.…”

Section: Discussionmentioning

confidence: 99%

Characterization of carbapenem non-susceptible Gram-negative Bacilli isolated from the feces of 10,000 inpatients in Southern China

Duan¹,

Li²,

Peng³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Carbapenem non-susceptible Gram-negative bacilli (CNS-GNB) were dominant pathogen causing clinical infections. The human intestine was important reservoir of GNB, but there were few studies to analysis the prevalence of fecal colonization with them. Methods Fecal samples were collected from hosiptal screening test for GNB was conducted by using home-made MacConkey agar. Antimicrobial susceptibility was determined by the automatic microbiology analyzer and drug-resistant genes were characterized by polymerase chain reaction assays and DNA sequencing. The whole genome sequencing were used to analysis the characteristic of genetic structure of the isolates. Results A total of 680 CNS-GNB were collected. Acinetobacter spp. were the dominant species (33.8%) of the 22 genera. Carbapenemase genes were identified in 307 isolates (45.1%), including 206 (30.3%) blaNDM; 51 (7.5%) blaVIM−2, 48 (7.1%) blaIMP, and seven (1.0%) blaKPC−2. The blaNDM genes were first detected in three isolates, Providencia vermicola, Achromobacter spp., and Cupriavidus gilardii. Co-existence of blaVIM and blaIMP genes was detected in five isolates; Achromobacter co-producing VIM and IMP has not been previously reported. The mcr-1 gene was identified in five strains of Acinetobacter and one strain of K. pneumoniae. In addition, we detected seven isolates harboring the blaAFM−1 gene, a novel metallo-β-lactamase gene. This was first genomic analysis of ST11 K. pneumoniae co-producing NDM-5 and mcr-1, which revealed that blaNDM-5 and mcr-1 are located on two different plasmids. The plasmid harboring blaNDM-5, which was composed of a typical IncX3-type backbone, and the mcr-1 gene, was located between an IS30-like element ISApl1 and a PAP2-like encoding gene in the IncHI2-type plasmid. Conclusions the overall prevalence of fecal carriage of CNS-GNB in 10,000 stool samples was 7.45%, and more than half of CNS-GNB produced carbapenemase. Most CNS-GNB cases were associated with infectious disease, multiple hospitalizations, or long-term care, and a high prevalence of underlying disease.

show abstract

“…The persistence of P. aeruginosa in a variety of habitats hinders treatment, [3] as does its resistance to carbapenem. [4,5] Carbapenem antibiotic is often the antibiotic of last resort in severe and life threatening infections caused by MDR gram negative bacteria. Understanding the resistance to carbapenem is important.…”

Section: Research In Molecular Medicine (Rmm)mentioning

confidence: 99%

Antimicrobial Effect of Multilayered Carbon Nanotubes on Multi-Drug-Resistant Pseudomonas aeruginosa

Mousavi

Nejad

Zandi

et al. 2019

RMM

View full text Add to dashboard Cite

Background: Pseudomonas aeruginosa is the primary cause of infection with impaired defense mechanisms. P. aeruginosa commonly causes nosocomial infections and is the most common pathogen isolated from patients hospitalized for longer than 1 week. We examined the antimicrobial effect of multilayered carbon nanotubes on multi-drugresistant. Materials and Methods: In this research, 20 clinical isolates collected at Motahari Hospital (Tehran, Iran) were compared with the standard (ATCC 27853) and identified as P. aeruginosa based on biochemical testing. Conventional disk diffusion assay demonstrated the methicillin resistance of the isolates. Minimal inhibitory concentrations for antibiotics and the multilayer CNTs were determined using the microdilution method. Single-walled CNTs were prepared and their efficacy and potential synergism with antibiotics was assessed. Results: Synergism against P. aeruginosa was evident for methicillin + single-walled CNTs. Conclusion: The inhibitory effect of single-walled CNTs and methicillin was synergistic against the growth of P. aeruginosa.

show abstract

Mechanisms of carbapenem resistance in endemic Pseudomonas aeruginosa isolates after an SPM-1 metallo-β-lactamase producing strain subsided in an intensive care unit of a teaching hospital in Brazil

Cited by 28 publications

References 29 publications

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Characterization of carbapenem non-susceptible Gram-negative Bacilli isolated from the feces of 10,000 inpatients in Southern China

Antimicrobial Effect of Multilayered Carbon Nanotubes on Multi-Drug-Resistant Pseudomonas aeruginosa

Contact Info

Product

Resources

About